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산업용 Saccharomyces cerevisiae에서 Ethionine 저항성 유전자의 발현
Expression of Ethionine Resistance Conferring Gene in an Industrial Strain of Saccharomyces cerevisiae

박정남   (전남대학교 생명과학부UU0001112  ); 이경희   (전남대학교 생명과학부UU0001112  ); 고현미   (전남대학교 생명과학부UU0001112  ); 서국헌   (전남대학교 생명과학부UU0001112  ); 진종언   (동강대학 피부미용과UU0000450  ); 이황희   (전남대학교 생명과학부UU0001112  ); 배석   (전남대학교 생명과학부UU0001112  );
  • 초록

    The ethionine resisconferring gene (ERCI) was constitutively expressed under the control of the alcohol dehydrogenase gene promoter (ADClp) and introduced into the chromosomes of an industrial polyploid strain of Saccharocerevisiae by using the 8-sequences of the Tyl retrotransposon as the recombination site. 8-Integrative cassette devoid of bacterial DNA sequences containing the ampicillin resistance gene was constructed that had the aureobasidin A resistance gene (AURl-C) as the selection marker and ERCl gene. The ERCl gene was also employed as the selection marker in the 8-integrative cassette lacking the A URl-C gene. Industrial Saccerevisiae transformed with these integrative cassettes exhibited strong resistance to DL-ethioncompared with nontransformants.


  • 주제어

    Ethionine resistance conferring gene .   industrial strain of Saccharomyces cerevisiae .   selection marker.  

  • 참고문헌 (30)

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  • 진종언 (18)

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  • Bai, Suk (31)

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