본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

배양액중의 유기영양물이 Chitinase 생산에 미치는 영향
Effects of Organic Nutrients on Chitinase Production in Minimal Media

장지윤   (조선대학교 식품영양학과UU0001180  ); 김인철   (조선대학교 식품영양학과UU0001180  ); 장해춘   (목포대학교 식품공학과UU0000552  );
  • 초록

    Four chitinase producing bacteria, Arthrobacter nicotinae CH4, Arthrobacter nicotinae CHI3, Arthrobacter sp. CH5 and Micrococcus sp. CH3, were isolated from small crabs and shrimps. We investigated the optimum medium condition for the production of enzyme and high cell mass. The preferable medium composition was as follows: colchitin 0.1 %(w/v), glycerol 0.25%(w/v) and yeast extract 0.05%(w/v) in minimal midium ( $K_{2}HPO_{4}$ 0.7 g/l, $KH_{2}PO_{4}$ 0.3 g/l, $MgSO_{4}{\cdot}5H_{2}O$ 0.5 g/l, $FeSO_{4}}{\cdot}7H_{2}O$ 0.01 g/l, $ZnSO_{4}$ 0.001 g/l, $MnCI_2$ 0.001 g/l, pH 7.0). This cell culture medium could be used directly as sample for measuring chitinase activity. Because it hardly conreducing sugar such as glucose (blank value=0), the detected reducing sugar can be considered as a chitinase reaction product. The results can be used for easy preparation method for determination of enzyme activity and analysis of enzyme-substrate reaction in step of screening of chitinase producing bacteria.


  • 주제어

    Colloidal chitin .   minimal media .   chitinase .   glycerol .   yeast extract.  

  • 참고문헌 (22)

    1. B. Hong, H. Yoon, D. Shin and H. Cho. 1996. Isolation of microorganism producing thermostable chitinase and some cultural characteristics for chitinase production. Kor. J. Appl. Microbiol. Biotech. 24: 560-566 
    2. Chang, J. Y., I. C. Kim and H. C. Chang. 2003. Microbial production of N-acetylglucosamine by Arthrobacter nicotianae. Kor. J. Food Sci. Technol. 35: 1188-1192 
    3. Knorr, D. 1984. Use of chitinous polymers in food. Food Technol. 38: 85-97 
    4. Patil, R.S., V. Ghormade and M.V. Deshpande. 2000. Chitinolytic enzymes: an exploration. Enzyme Microbiol Technol. 26: 473-483 
    5. Rupley, J.A. 1964. The hydrolysis od chitin by concentrated hydrochloric acid, and the preparation of low molecluar weight substrates for lysozyme. Biochem. Biophys. Acta. 83: 245-255 
    6. Sauki, K., T. Uchiyama, Y. Matahira and F. Nomjo. 1991. Immobilization of chitinolytic enzymes and continuous production of N-acetylglucosamine with the immobilized enzyme. J. Ferment. Technol. 72: 168-172 
    7. Berger, L. R. and D. M. Reynolds. 1958. Chitinase system of a strain of Streptomyces griseus. Biochem. Biophys. Acta. 29: 522-534 
    8. K. Kim, C. Lee and K. Lee. 1997. Isolation of Aeromonas hydrophila and chitinolytic properties. Kor. J. Appl. Microbiol. Biotech. 25: 151-158 
    9. Somogyi. A.M. 1952. A new regent for determination of sugar. J. Biol. Chem. 195: 19-24 
    10. Reynolds, D.M. 1954. Extracellular chitinase from a Streptomyces sp. J. Gen. Microbiol. 11: 150-159 
    11. Hong, B. S., H. G. Yoon, D. H. Shin and H. Y. Cho. 1996. Isolation of microorganism producing thermostable chitinase and some cultural characteristics for chitinase production. Kor. J. Appl. Microbiol. Biotechnol. 24: 560-566 
    12. Lee, K.P., C.N. Kim, J.H. Yu and D.H. Oh. 1990. The production and purification of chitinase from Aeromonase salmonicida YA7-625. Kor. J. Appl. Microbiol. Biotechnol. 18: 599-606 
    13. B. Hang, H. Yoon, D. Shin and H. Cho. 1996. Purification and characterization of thermostable chitinase from Bacillus lichenifomis KFB-C14. Kor. J. Appl. Microbiol. Biotech. 24: 567-573 
    14. Nelson, N.A. 1944. A photometric adsorption of somogyi method for determination of glucose. J. Biol. Chem. 153: 376-380 
    15. Friedman, S. J. and P. Skehan. 1980. Membrane-ative drugs potentiate the killing of tumor cells by D-glucosamine. Proc. Nat Acad Sci. USA. 77: 1172-1176 
    16. Lee, K., C. Kim, J. Yu and D. Oh. 1990. The production and purification of chitinase from Aeromonas salmonicida YA7- 625. Kor. J. Appl. Microbiol. Biotech. 26: 515-522 
    17. Lee, S.M. 1993. Identification and cultural characterization of Streptomyces lydicus G-23 for producing chitinase. Kor. J. Appl. Microbiol. Biotechnol. 23: 187-196 
    18. Abdel-Naby, M. A. and D. Y. Kwon. 1992. Enzymatic hydrolysis of pretreated chitin by Aspergillus carmeus chitinase. J. Microbiol. Biochem. 2: 197-203 
    19. http://www.iherb.com/ngg.html 
    20. Hsu, S. C. and L. Lockwood. 1975. Powdered chitin agar as a selective medium for enumeration of Actinomyces in water soil. Appl. Microbiol. 29: 422-429 
    21. Jeong, E. and Y. Lee. 1995. Isolation of microorganism producing of chitinase and its enzymatic characteristics. Kor. J. Appl. Microbiol. Biotechnol. 23: 187-196 
    22. Lee, J., D. Kim, J. D. and S. Kim. 1998. Purification and characterization of chitinase from antagonistic bacteria Pseudomonas sp. 3098. Kor. J. Appl. Microbiol. Biotech. 26: 515-522 

 저자의 다른 논문

  • 장지윤 (7)

    1. 1999 "E. coli에서 탄수화물원에 따른 Lactococcal /beta-galactosidase의 발현" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 27 (3): 260~265    
    2. 2001 "Bacillus licheniformis cy2가 생산하는 박테리오신의 특성" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 30 (3): 410~414    
    3. 2001 "Bacillus subtilis cx1이 생산하는 박테리오신의 특성" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 29 (1): 50~55    
    4. 2002 "김치로부터 Lactobacillus plantarum 생육저해 박테리오신 생산균주의 분리 및 박테리오신 생산의 유도효과" 한국식품과학회지 = Korean journal of food science and technology 34 (2): 311~318    
    5. 2003 "Arthrobacter nicotianae에 의한 N-acetylglucosamine의 생산" 한국식품과학회지 = Korean journal of food science and technology 35 (6): 1188~1192    
    6. 2007 "Production of ${\gamma}-Aminobutyric$ Acid (GABA) by Lactobacillus buchneri Isolated from Kimchi and its Neuroprotective Effect on Neuronal Cells" Journal of microbiology and biotechnology 17 (1): 104~109    
    7. 2014 "김치제조시 사용되는 천일염이 김치의 장기저장에 미치는 영향" 한국식품과학회지 = Korean journal of food science and technology 46 (4): 456~464    

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기