본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of bacteriology and virology : JBV v.34 no.4, 2004년, pp.303 - 310   피인용횟수: 1
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Prevalence of CTX-M Extended-spectrum Beta-lactamases in Clinical Isolates of Enterobacteriaceae in Korea

Kim, Jung-Min  
  • 초록

    The evolution and dissemination of extended-spectrum $\beta$ -lactamases (ESBL) have compromised the clinical use of third-generation cephalosporins worldwide. Although most ESBLs belong to the TEM and SHV $\beta$ -lactamase families, the members of CTX-M, a novel ESBL family, are increasing worldwide in Gram-negative bacteria. We examined the prevalence of CTX-M ESBL in clinical isolates of the family Enterobacteriaceae collected from three university hospitals located in three different cities in Korea. Among a total of 603 isolates collected, 163 isolates (27.0%) revealed ${\geq}2\;{\mu}g/ml$ of MIC against cefotaxime, and 93 isolates (15.4%) produced ESBL confirmed by the double disk synergy test. Among 93 ESBL-producing isolates, $bla_{CTX-M}$ genes were detected in 41 isolates by PCR method and they included 1 isolate of C. freundii, 3 of E. aerogenes, 2 of E. cloacae, 17 of E. coli, 9 of K. pneumoniae, and 9 of S. marcescens. Thus, the overall prevalence of CTX-M ESBL-producing isolates among the family Enterobacteriaceae was 6.8% (41 of 603 isolates) and the proportion of CTX-M-producers among the ESBL-producing isolates was 44.1 % (41 of 93 isolates). Further determination of the $bla_{CTX-M}$ subtype by nucleotide sequencing revealed $bla_{CTX-M-3}$ in 17, $bla_{CTX-M-15}$ in 11, $bla_{CTX-M-14}$ in 9, and $bla_{CTX-M-9}$ in 4 isolates. To our knowledge, this is the first report on the dissemination of CTX-M ESBL among clinical isolates of the family Enterobacteriaceae in Korea.


  • 주제어

    CTX-M-3 .   CTX-M-15 .   CTX-M-9 .   CTX-M-14 .   Enterobacteriaceae.  

  • 참고문헌 (16)

    1. Cao V, Lambert T, Courvalin P: Co1E1-like plasmid pIP843 of Klebsiella pneumoniae encoding extended spectrum beta-lactamase CTX-M-17. Antimicrob Agents Chemother 46: 1212-1217, 2002 
    2. Chanawong A, M'Zail FH, Heritage J, Xiong JH, Hawkey PM: Three cefotaximases, CTX-M-9, CTX-M-13, and CTX-M-14, among Enterobacteriaceae in the People's Republic of China. Antimicrob Agents Chemother 46: 630-637, 2002. 
    3. Gazouli M, Tzelepi E, Markogiannakis A, Legakis NJ, Tzouvelekis LS: Two novel plasmid-mediated cefotaxime-hydrolysing beta-lactamases (CTX-M-5 and CTX-M-6) from Salmonella typhimurium. FEMS Microbiol Lett 165: 289-293, 1998 
    4. Jeong YS, Lee JC, Kang HY, Yu HS, Lee EY, Choi CH, Tae SH, Lee YC, Cho DT, Seol SY: Epidemiology of nalidixic acid resistance and TEM-l- and TEM-52-mediated ampicillin resistance of Shigella sonnei isolates obtained in Korea between 1980 and 2000. Antimicrob Agents Chemother 47: 3719-3723, 2003 
    5. Bonnet R, Sampaio JL, Labia R, De Champ C, Sirot D, Chanal C, Sirot J: A novel CTX-M beta-lactamase (CTX-M-8) in cefotaxime-resistant Enterobacteriaceae isolated in Brazil. Antimicrob Agents Chemother 46: 1936-1942, 2002. 
    6. Gniadkowski M, Schneider I, Palucha A, Jungwirth R, Mikiewicz B, Bauernfeind A: Cefotaxime-resistant Enterobacteriaceae isolates from a hospital in Warsaw, Poland: identification of a new CTX-M-3 cefotaximehydrolyzing $\beta$-lactamase that is closely related to the CTX-M-1/MEN-1 enzyme. Antimicrob Agents Chemother 42: 827-832, 1998. 
    7. Kariuki S, Corkill JE, Revathi G, Musoke R, Hart CA: Molecular characterization of a novel plasmidencoded cefotaximase (CTX-M-12) found in clinical Klebsiella pneumoniae isolates from Kenya. Antimicrob Agents Chemother 45: 2141-2143, 2001 
    8. Barthelemy M, Peduzzi J, Bernard H, Tancrede C, Labia R: Close amino-acid sequence relationship between the new plasmid-mediated extended-spectrum $\beta$-lactamase MEN-1 and chromosomally encoded enzymes of Klebsiella oxytoca. Biochim Biophys Acta 1122: 15-22, 1992. 
    9. Baraniak A, Fiett J, Sulikowska A, Hryniewicz W, Gniadkowski M: Countrywide spread of CTX-M-3 extended-spectrum $\beta$-lactamase-producing microorganisms of the family Enterobacteriaceae in Poland. Antimicrob Agents Chemother 46: 151-159, 2002 
    10. Bauerfeind A, Grimm H, Schweighart S: A new plasmidic cefotaximase in a clinical isolate of Escherichia coli. Infection 18: 294-298, 1990 
    11. Bonnet R: Growing group of extended-spectrum $\beta$-lactamases: the CTX-M-enzymes. Antimicrob Agents Chemother 48: 1-14, 2004 
    12. Karim A, Poirel L, Nagarajan S, Nordmann P: Plasmid-mediated extended-spectrum $\beta$-lactamase (CTX-M-3-like) from India and gene association with insertion sequence ISEcp1. FEMS Microbiol Lett 201: 237-241, 2001 
    13. Jeong SH, Bae IK, Lee JH, Sohn SG, Kang GH, Jeon GJ, Kim YH, Jeong BC, Lee SH: Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey. J Clin Microbiol 42: 2902 1906, 2004. 
    14. Cao V, Lambert T, Nhu DQ, Loan HK, Hoang NK, Hawkey PM: Distribution of extended-spectrum betalactamase in clinical isolates of Enterobacteriaceae in Vietnam. Antimicrob Agents Chemother 46: 3739-3743, 2002 
    15. Bonnet R, Dutour C, Sampaio JLM, Chanal C, Sirot D, Labia R, Sirot J: Novel cefotaximase (CTX-M-16) with increased catalytic efficiency due to substitution Asp-240 to Gly. Antimicrob Agents Chemother 45: 2269-2275, 2000. 
    16. Jeong SH, Bae IK, Kwon SB, Lee JH, Jung Hl, Song JS, Jeong BC, Kim SJ, Lee SH: Investigation of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli in Korea. Lett Appl Microbiol 39: 41-47, 2004 
  • 이 논문을 인용한 문헌 (1)

    1. 2014. "" Journal of bacteriology and virology : JBV, 44(1): 44~51     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기