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The plant pathology journal v.20 no.4, 2004년, pp.247 - 251  
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Early Detection of Epiphytic Anthracnose Inoculum on Phyllosphere of Diospyros kaki var. domestica

Lee, Jung-Han    (Department of Applied Biology & Environmental Sciences, Gyeongsang National Univesity   ); Han, Ki-Soo    (Department of Applied Biology & Environmental Sciences, Gyeongsang National Univesity   ); Lee, Sun-Cheol    (Department of Applied Biology & Environmental Sciences, Gyeongsang National Univesity   ); Shim, Chang-Ki    (Department of Applied Biology & Environmental Sciences, Gyeongsang National Univesity   ); Bae, Dong-Won    (Central Laboratory, Gyeongsang National Univesity   ); Kim, Dong-Kil    (Research Institute of Life Science, Gyeongsang National Univesity   ); Kim, Hee-Kyu    (Research Institute of Life Science, Gyeongsang National Univesity  );
  • 초록

    We developed a polyclonal antibody (PAh) based- ELISA system to accurately and rapidly monitor inocula on plant surface before onset of anthracnose. Titer of mouse antisera against conidia of Colletotrichum gloeosporioides was determined by using indirect ELISA. It was high enough to be detectable up to ${\times}$ 12,800 dilutions. Absorbance readings exceeded (1.5even at a 10 $^{-5}$ dilution. Sensitivity of PAb was precise enough to detect spore concentration as low as 50 conidia/well by indirect ELISA. PAb1 and PAb2 proved to be very sensitive and highly specific to the target pathogen, C. gloeosporioides, apparently discriminating other unrelated pathogens, or epiphytes. Absorbance values for original isolate exceeded 1.0, but no reaction was detected with other isolates, except three other anthracnose fungi: C. gloeosporioides (pepper strain), Glomerella cingulata (apple strain) and C. lagenarium. Our data suggest that PAb1 and PAb2 bind with the protein epitope that partially contains residues of amino acid, arginine, and Iysine. This kit fulfills the require-ments for detecting inoculums before infection and during onset of anthracnose on sweet persimmon.


  • 주제어

    Diospyros kaki .   anthracnose .   indirect ELISA.  

  • 참고문헌 (10)

    1. Clark, M. F. 1981. Immunosorbent assays in plant pathology. Annual Rev. Phytopathology 19:83-106 
    2. Velicheti, R. K., Lamison, C., Brill, L. M. and Sinclair, J. B. 1993. Immunodetection of Phomopsis species in asymptomatic soybean plants. Plant Dis. 77:70-73 
    3. Sundaram, S., Plasencia, J. and Banttari, E. E. 1991. Enzymelinked immunosorbent assay for detection of verticillium spp. Using antisera produced to V. dahliae from potato. Phytopathology 81: 1485-1489 
    4. Kwon, J. H., Kang, S. W. and Park, C. S. 2000. Cultural characteristics of Colletotrichum gloeosporioides causing Anthracnose ofpersimmon. Plant disease Research 6:48-50 
    5. Ryu, H. Y, Lee, Y H., Cho, W. D., Kim, W. K., Myung, I. S. and Jin, K. S. 1993. Compendium offiuit tree diseases with colour plates. Agricultural Sciences Institute, Suwon. Gyeonggido Printing Industry Cooperative 286pp 
    6. Somai, B. M. and Keinath, A. P. 2002. Development of PCRELISA for detection and differentiation of Didymella bryoniae from related phoma species. Plant Dis. 86:710-716 
    7. Lee, Y H. and Shin, H. D. 1998. List of plant diseases in Korea. 3d edition p. 203 Korean Society of Plant Pathology World Science Co. 436pp 
    8. Singh, U., Trevors, C. M., Boer, S. H. and Janse, J. D. 2000. Fimbrial- specific monoclonal antibody-based ELISA for European potato strains of Envinia chrysanthemi and comparison to PCR. Plant Dis. 84:443-448 
    9. Meyer, U. M., Spotts, R. A. and Dewey, F. M. 2000. Detection and quantification ofBotrytis cinerea by ELISA in pear stems during cold storage. Plant Dis. 84: 1099-1103 
    10. Kobayashi, T. and Katumoto, K. 1992. Taxonomic Revision and References for Imperfect Fungal Pathogens p. 590-591. (In Japanese) in Chapter 6 of the Compendium of Plant Pathogenic Fungi. 685pp. Japanese Rural Education Association. Ann Shin Printing Co. Tokyo Japan 

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