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The plant pathology journal v.20 no.4, 2004년, pp.293 - 296   피인용횟수: 8
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Inhibitory Effects of Acinetobacter sp. KTB3 on Infection of Tobacco mosaic virus in Tobacco Plants

Kim, Young-Sook    (Major in Plant Pathology and Cell Technology, Department of Agricultural Biology, Chungnam National University   ); Hwang, Eui-ll    (Bio Research Group, KT&G Central Research Institute   ); O, Jeong-Hun    (Bio Research Group, KT&G Central Research Institute   ); Kim, Kab-Sig    (Bio Research Group, KT&G Central Research Institute   ); Ryu, Myong-Hyun ;    (Bio Research Group, KT&G Central Research Institute   );
  • 초록

    During the screening of antiviral substances having inhibitory effects on Tobacco mosaic virus (TMV) infection on tobacco plants, we found a bacterial isolate KTB3, and identified it as Acinetobacter sp. which strongly inhibited the infection of TMV When the culture filtrate from KTB3 was applied on the upper surface of the Xanthi-nc tobacco leaves at the same time, or 24 hours before TMV inoculation, almost complete inhibition was achieved. Likewise, 86% inhibition was achieved, when the culture filtrate was applied on the underside of the leaves. In field trials, transmission of TMV from diseased seedlings to healthy ones during transplanting work was reduced by 92%, when the culture filtrate was sprayed onto the tobacco seedlings, cv. NC82, 24 hours before transplanting. No toxic effect was observed on the tobacco plants. Antiviral substance from the culture filtrate was purified by ethanol precipitation, dialysis, DEAE-cellulose, and Sephadex G75 gel column chromatography. The partially purified active material which showed positive color reaction to sugar and protein inhibited TMV infection by 60% at 1 ${\mu}$ g/ml.


  • 주제어

    Acinetobacter sp.   .   Inhibitory effects .   TMV infection .   Tobacco plants.  

  • 참고문헌 (10)

    1. Aoki, M., Tan, M., Fukushima, A., Hieda, T., Kubo, S., Takabayashi, M., Ono, K. and Mikami, Y. 1993. Antiviral substances with systemic effects produced by basidiomycetes such as Fomes fomentarius. Biosci. Biotech. Biochem. 57:278-282 
    2. Yang, P., Vauterin, L., Vancaneyt, M., Swing, J. and Kersters, K. 1993. analysis of the genus Xanthomonas. Syst. Appl. Microbioi. 16: 47-71 
    3. Yeo, W. H., Kim, Y. H., Park, E. K. and Kim, S. S. 1997. Physicochemical characteristics and antiviral activity of ASA, an antibiotic produced by actinomycetes B25. Korean J Plant Pathol' 13:63-68 
    4. Stevens, W. A. and Reynolds, T. 1992. Plant virus inhibitors from members of the Polygonaceae. Biomedical Letters 47:269273 
    5. Ito, Y., Seki, I., Tanifuji, S. and Hiramatsu, A. 1992. Inhibition of protein synthesis by antiviral protein from Yucca recurvifolia leaves. Biosci. Biotech. Biochem. 57:518-519 
    6. Hudson, J. B. 1990. Antiviral compounds from plants. CRC Press Inc. Boca Raton, Florida, USA. 200pp 
    7. Maidak, B. L., Olsen, G J., Larsen, N., Overbeek, R., McCaughey, M. J. and Woese, C. 1997. The RDP (Ribosomal Database Project). Nucl. Acids Res. 25:109-111 
    8. Klement, Z., Kiraly, J. and Pozsar, I. 1966. Suppression of virus multiplication and local lesion production in tobacco following inoculation with a saprophytic bacterium. Acta Phytopathol. Acad. Sci. Hung. 1: 11-18 
    9. Yumoto, I., Yamazaki, K., Sawabe, T., Nakano, K., Kawasaki, K., Ezura, Y. and Shinano, H. 1998. Bacillus horti sp. nov., a new Gram-negative akaliphilic Bacillus. Int. J Sys. Bacteriol. 48: 565-571 
    10. Kreig, N. R. and Halt, J. G 1984. Bergey's Manual of Systematic Bacteriology. Williams and Wilkins Co., Baltimorel London 
  • 이 논문을 인용한 문헌 (8)

    1. 2005. "" The plant pathology journal, 21(3): 262~269     
    2. Ipper, Nagesh S. ; Lee, Seon-Hwa ; Suk, Jung-Ki ; Shrestha, Anupama ; Seo, Dong-Uk ; Park, Duck-Hwan ; Cho, Jun-Mo ; Park, Dong-Sik ; Hur, Jang-Hyun ; Lim, Chun-Keun 2006. "Isolation and Evaluation of an Antiviral Producing Serratia spp. Strain Gsm01 against Cucumber mosaic virus in Korea" 농약과학회지 = The Korean journal of pesticide science, 10(4): 344~350     
    3. Kim Mi-Soon ; Jung Min-Young ; Kim Yun-Sung ; Jang Cheol ; Hwang In-Cheon ; Ryu Ki-Hyun ; Choi Jang-Kyung 2006. "Inhibitory Effect on Infection of Plant Viruses by Filtrate Powder from Culture Broth of Acinetobacter sp. and Its Mode of Action" Research in plant disease = 식물병연구, 12(2): 91~98     
    4. 2008. "" The plant pathology journal, 24(3): 328~336     
    5. 2009. "" The plant pathology journal, 25(1): 77~85     
    6. 2009. "" Journal of microbiology and biotechnology, 19(3): 250~256     
    7. Kwon, Soon-Bae ; Bae, Seon-Hwa ; Choi, Jang-Kyung ; Lee, Sang-Yong ; Kim, Byung-Sup ; Kwon, Yong-Soo 2010. "Inhibitory Effects of PLM-WE1 Formulated from Extract of Phellinus linteus Mycelium against Plant Viruses Infection and Identification of Active Compound" Research in plant disease = 식물병연구, 16(3): 259~265     
    8. Kwon, Soon-Bae ; Lee, Hye-Myong ; Kim, Byung-Sup ; Choi, Jang-Kyung 2010. "Inhibitory Effect of Extracts from 33 Medicinal Herbs Against TMV and CMV Infection" 농약과학회지 = The Korean journal of pesticide science, 14(3): 280~283     

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