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Journal of plant biotechnology v.6 no.4, 2004년, pp.229 - 233  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Partial Desiccation of Embryogenic Calli Improves Plant Regeneration in Sugarcane (Saccharum Spp.)

Desai Neetin Shivajirao    (Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center   ); Suprasanna Penna    (Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center   ); Bapat Viswas Ananat    (Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center  );
  • 초록

    Partial desiccation of embryogenic calli cultures or somatic embryos leads to different physiological changes and maturation of somatic embryos, leading to improved plant regeneration. Embryogenic calli was induced from immature inflorescence segments and young leaf rolls of sugarcane (Saccharum officinarum hybrids CoC-671) on Murashige and Skoog's basal medium enriched with different concentrations of 2,4-D ( $1-4\;\cal{mg/l}$ ), L-glutamine ( $100\cal{mg/l}$ ), malt extract ( $100\cal{mg/l}$ ), casein hydrolysate ( $1000\;\cal{mg/l}$ ) and coconut milk ( $5\%$ ) and solidified with $0.2\%$ gel rite. The embryogenic calli were subjected to desiccation for 1-8 h. Desiccation of the calli for 6-7 h resulted in enhancement of plant regeneration frequency ( $83-96\%$ ) as compared to control ( $12\%$ ). Plantlets exhibited vigorous growth to maturity in the greenhouse. Partial desiccation of embryogenic calli offers as a simple method for improving plant regeneration frequency in sugarcane.


  • 주제어

    Saccharum spp.   .   embryogenic calli .   desiccation .   plant regeneration.  

  • 참고문헌 (24)

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