본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of plant biotechnology v.6 no.4, 2004년, pp.235 - 239  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

An Improved Plant Regeneration Protocol using Cotyledonary Explants from Inbred Lines of Chinese Cabbage (Brassica rapa ssp. pekinensis)

Yang Zhi Hong    (Genome Research Center, Department of Horticulture, Chungnam National University   ); Jin Hua    (Genome Research Center, Department of Horticulture, Chungnam National University, Institute of Biological Resources and Environmental Research, Da Lian Nationalties University   ); Plaha Prikshit    (Genome Research Center, Department of Horticulture, Chungnam National University, Advanced Centre of Hill Bioresources & Biotechnology, HP Agricultural University   ); Woong Bae Tae    (Department of Horticulture, College of Agriculture and Life Science, Cheju National University   ); Jiang Guo Bin    (Institute of Biological Resources and Environmental Research, Da Lian Nationalties University   ); Woo Jong Gyu    (National Horticultural Research Institute, RDA   ); Yun Han Dae    (Division of Applied Life Science, Gyeongsang National University   ); Lim Yong Pyo    (Genome Research Center, Department of Horticulture, Chungnam National University   ); Lee Hyo Yeon    (Department of Horticulture, College of Agriculture and Life Science, Cheju National University  );
  • 초록

    We studied the effect of genotype, explant, age of explant, medium (plant growth regulators and gelling agents), and standardized an efficient regeneration protocol for inbred lines of Chinese cabbage (Brassica rapa ssp. pekinensis). Of the different concentrations of NAA and BA tested, the combination of $5\;\cal{mg/L}\;BA\;and\;0.5\;\cal{mg/L}$ NAA gave the highest frequency of shoot regeneration. The cotyledonary explants had more shoot regeneration frequency ( $\ge40\%$ ) than the hypocotyl ex-plants. Besides, the cotyledonary explants, excised from the 4-day old seedlings, showed higher shoot regene-ration ( $56.7\%$ ). Of the three gelling agents and their concentrations used, 16 g/L agar was found to be the best for shoot regeneration. Shoot regeneration frequency in-creased significantly by supplementing the medium with $4\;\cal{mg/L}\;of\;AgNO_3$ MS medium devoid of NAA showed higher frequency of rooting in the regenerated shoots than the ones supplemented with NAA. Our improved regeneration protocol will be especially useful for the genetic transformation of Chinese cabbage inbred lines to develop transgenic hybrids.


  • 주제어

    Chinese cabbage .   Brassica rapa ssp .   pekinensis .   explants .   shoot regeneration.  

  • 참고문헌 (14)

    1. Beyer EM Jr (1976) A potential inhibitor of ethylene action in plants. Plant Physiol 58: 268-271 
    2. Cho YN, Park SY, Noh TK, Song MJ, Park YS, Min BW (2003) Transformation of Chinese cabbage with L-Gluno- $\gamma$ - Lactone Oxidase (GLOase) - encoding gene using Agrobacterium tumefaciencs. Kor J Hort Sci 21: 9-13 
    3. Christey MC, Sinclair BK, Braun RH, Wyke L (1997) Regeneration of transgenic vegetable brassicas (Brassica oleracea and B. campestris) via Ri-mediated transformation. Plant Cell Rep 16: 587-593 
    4. Moloney MM, Walker JM, Sharma KK (1989) High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep 7 104-106 
    5. Narasimhulu SB, Chopra VL (1988) Species specific shoot regeneration response of cotyledonary explants of Brassicas. Plant Cell Rep 7: 104-106 
    6. Takasaki T, Hatakeyama K, Ojima K, Watanable M, Toriyama K, Hinata K (1996) Effects of various factors (hormone combinations, genotypes and antibiotics) on shoot regeration from cotyledon explants in Brassica rapa L. Plant Tiss Cult Lett 13: 177-180 
    7. Tang GX, Zhou WJ, Li HZ, MaoBZ, He ZH, Yoneyama K (2003) Medium, explant and genotype factors influencing shoot regeneration in oilseed Brassica species. J Agron Crop Sci 189: 347-350 
    8. Burnett L, Arnoldo M, Yarrow S, Huang B (1994) Enhancement of shoot regeneration from cotyledon explants of Brassica rapa ssp. oleifera through pretreatment with auxin and cytokinin and use of ethylene inhibitors. Plant Cell Tiss Org Cult 37: 253-256 
    9. Chi GL, Barfield DG, Sim GE, Pua EC (1990) Effect of Ag$NO_3$ and aminoethoxyvinylglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes. Plant Cell Rep 9: 195-198 
    10. Hachey JE, Sharma KK, Moloney MM (1991) Efficient shoot regeneration of Brassica campestris using cotyledon explants cultured in vitro. Plant Cell Rep 9: 549-554 
    11. Zhang F-L, Takahata Y, Xu J-B (1998) Medium and genotype factors influencing shoot regeneration from cotyledonary explants of Chinese cabbage (Brassica rapa L. ssp. pekinensis). Plant Cell Rep 17: 780-786 
    12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays in tobacco tissue culture. Physiol Plant 15: 473?493 
    13. Murata M, Orton TJ (1987) Callus initiation and regeneration capacities in Brassica species. Plant Cell Tiss Org Cult 11: 111-123 
    14. Cho HS, Cao J, Ren JP, Earle ED (2001) Control of lepidopteran insect pests in transgenic Chinese cabbage transformed with synthetic Bacillus thuringiensis cry1C gene. Plant Cell Rep 20 1-7 

 저자의 다른 논문

  • Lim, Yong-Pyo (67)

    1. 1993 "고려인삼(Panax ginseng C.A. Meyer)의 엽록체 DNA 분리 및 특성조사" 고려인삼학회지 = Korean journal of ginseng science 17 (1): 39~44    
    2. 1993 "Randomly Amplified Polymorphic DNA (RAPD) 기술을 이용한 고려인삼의 유전분석을 위한 Primer 선발 및 변종별 비교" 고려인삼학회지 = Korean journal of ginseng science 17 (2): 153~158    
    3. 1994 "Agrobacterium tumefaciens를 매개로 한 옥수수 유동유전자 Ac 및 Ds에 의한 서양고추냉이 (Armoracia rusticana)의 형질전환" 식물조직배양학회지 = Korean journal of plant tissue culture 21 (6): 319~326    
    4. 1997 "Particle Bombardment 방법을 이용한 인공 씨감자의 형질전환" 식물조직배양학회지 = Korean journal of plant tissue culture 24 (2): 87~91    
    5. 1997 "옥수수 R-mb 유전자의 유전분석과 그의 구조" 식물조직배양학회지 = Korean journal of plant tissue culture 24 (3): 161~165    
    6. 1998 "중이온 빔 조사에 의한 담배의 돌연변이 유도와 내염성 식물의 선발" 식물조직배양학회지 = Korean journal of plant tissue culture 25 (2): 103~107    
    7. 1998 "Agrobacterium tumefaciens를 이용한 bialaphos 저항성 형질전환 벼의 개발" 식물조직배양학회지 = Korean journal of plant tissue culture 25 (4): 283~288    
    8. 1998 "RT-PCR을 이용한 수박 Cucumber Green Mottle Mosaic Virus의 효율적인 진단 및 외피단백질 유전자의 클로닝" 식물조직배양학회지 = Korean journal of plant tissue culture 25 (6): 519~524    
    9. 2000 "Cloning and Characterization of UV-B Inducible Chalcone Synthase from Grape Cell Suspension Culture System and Its Expression Compared with Stilbene Synthase" Journal of photoscience : an international journal officail organ of the korean society of photoscience 7 (2): 53~58    
    10. 2000 "사과 자엽의 기내 식물체 재분화와 배양 광조건 및 절편체 극성과의 관계" 식물조직배양학회지 = Korean journal of plant tissue culture 27 (1): 19~23    

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기