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Journal of plant biotechnology v.6 no.4, 2004년, pp.241 - 246  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Stimulation of In Vitro Bulblet Growth by the Addition of Liquid Medium in Lilium Oriental Hybrid 'Casablanca'

Han Bong-Hee    (Horticulture Research Institute   ); Suh Eun-Jung    (Horticulture Research Institute   ); Choi Sung-Lyeol    (Horticulture Research Institute   ); Yae Byeoung-Woo    (Research Coordination Division, Rural development Administration   ); Yu Hee-Ju    (Horticulture Research Institute   ); Goo Dae-Hoe    (Horticulture Research Institute  );
  • 초록

    The bulb scales and shoot sections ( $7\;\cal{mm}\;\times\;15\;\cal{mm}$ ) of Lilium oriental hybrid 'Casablanca' were cultured to compare bulblet growth in vitro. Shoots were induced from in vitro grown bulbscales on MS medium with $1.0\;\cal{mg/L}\;BA,\;0.5\;\cal{mg/L}$ IAA, and 30 g/L sucrose. The regenerated shoots were cut into shoot sections, and cultured on MS medium with $2.0\;\cal{mg/L}\;BA,\;0.5\;\cal{mg/L}$ IAA and 30 g/L sucrose for shoot proliferation. Culture of shoot sections stimulated bulblet growth significantly than the bulb scales on MS medium with 60 g/L sucrose. However, the bulblets from shoot sections did not reach ideal size to produce stems with several leaves. Therefore, liquid medium was added into the same vessels to stimulate bulblet growth further. After shoot sections were cultured on MS medium with 60 g/L sucrose and 2 g/L activated charcoal for two months in dark, $20\;\cal{ml}$ liquid media containing various concentrations of sucrose and MS salts were added. Two months later, the added liquid medium stimulated bulblet growth remarkably as compared to bulblets grown without added liquid medium. The added $25\;\cal{ml}$ liquid medium containing 120 g/L sucrose and double strength of MS salts were the most effective for growth of in vitro bulblets. More than $94\%$ bulblets produced by this method sprouted stems with several leaves after cold treatment at $5^{\circ}C$ for three months.


  • 주제어

    Activated charcoal .   shoot formation .   sucrose.  

  • 참고문헌 (19)

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    2. Stimart DP, Ascher PO (1978) Tissue culture of bulb scale sections for asexual propagation of Lilium longiflorum Thunb. J Amer Soc Hor Sci 103:182-184 
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    4. Gerrits ML, Miller W, Kipnis HL, Kolloffel C, Cross T, De Klerk G.J (1997) Bulb growth in lily regenerated in vitro. Acta Hort 430:267-273 
    5. Han BH, Yae BW, Goo DH, Ko JY (1999a) Effects of growth regulators and light on the formation and proliferation of bulblets with swollen basal plate from in vitro culture of bulbscales in Lilium oriental hybrid 'Casablanca'. J Kor Soc Hort Sci 40:463-466 
    6. Lian ML, Chakrabarty D, Peak KY (2002) Growth and uptake of sucrose and mineral by bulblets of Lilium oriental hybrid 'Casablanca' during bioreactor culture. J Hort Sci Biotech 77:253-257 
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    15. Han BH, Yae BW, Goo DH, Ko JY (1999c) Effect of inorganic salts in MS medium, sucrose, and activated charcoal on bulblet formation from in vitro bulb scales in Lilium oriental hybrid 'Casablanca'. Kor J Plant Tiss Cult 26:103-107 
    16. Matsuo E, Ohkurano T, Arisumi K, Sakata T (1986) Scale bulblet malformations seen in Lilium longiflorum during scale propagation. HortSci 21:150 
    17. Van Aartrijk J, Blom-Barnhoorn G.J (1980) Effects of sucrose, mineral salts, and some organic substances on the additious regeneration in vitro of plantlets from bulb-scale tissue of Lilium speciosum . Rubrum'. Acta Hort 109:297-302 
    18. Yae BW, Han BH, Goo DH (2001) Dormancy breaking and in vitro growth of in vitro bulblets in Liliun oriental hybrid 'Casablanca' . J Kor Soc Hort. Sci 42:99-102 
    19. Marinangeli P, Curvetto N (1998) Growth in soil of micro- and macro-propagated Lilium bulblets. Phyton-International J Expt Bot 63:237-244 

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