본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of plant biotechnology v.6 no.4, 2004년, pp.265 - 268   피인용횟수: 1
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Effect of Inoculum Size on Biomass Accumulation and Ginsenoside Production by Large-Scale Cell Suspension Cultures of Panax ginseng

Thanh Nguyen Trung    (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University   ); Murthy Hosakatte Niranjana    (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Department of Botany, Karnatak University   ); Yu Kee-Won    (CBN Biotech Co. Ltd.   ); Jeong Cheol Seung    (CBN Biotech Co. Ltd.   ); Hahn Eun-Joo    (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University   ); Paek Kee-Yoeup    (Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University  );
  • 초록

    Cell growth and ginseng saponin production by large-scale suspension (bioreactor) cultures of Panax ginseng were investigated under various inoculum sizes. Cell growth was low at an inoculum size of 40 g FW/L, and the maximum cell growth was obtained with increasing inoculum size up to 100 g FW/L. The cell density of 333 g FW/L and 12.7 g DW/L was obtained at inoculum size of 100 g FW/L after 30 days of cultivation. Maximum saponin production of $4.40\;\cal{mg/g}$ DW was achieved at 60 g FW/L of inoculum size. Thus, inoculum size 60 g FW/L was suitable for optimum biomass accumulation as well as saponin production during bioreactor cultivation of ginseng suspension cells.


  • 주제어

    Bioreactor .   ginseng .   inoculum size .   saponin .   suspension culture.  

  • 참고문헌 (14)

    1. Matsubara K, Kitani S, Yoshioka T, Morimoto T, Fujita Y, Yamada Y (1989) High-density culture of Coptis japonica cells increases berberine production. J Chem Tech Biotechnol 46: 61-69 
    2. Rokem JS, Goldberg I (1985) Secondary metabolites from plant cell suspension cultures: Methods for yield improvement. In: Mizrahi A, van Wenzel AL eds. Advances in Biotechnological Processes 4. New York: Alan R. Liss, pp 241-274 
    3. van Gulik WM, Nuutila AM, Vinke KL, ten Hoopen HJG, Heijen JJ (1994) Effect of carbon dioxide, air flow rate, and inoculation density on the batch growth of Catharanthus roseus cell suspensions in stirred fermentors. Biotechnol Prog 10: 335-339 
    4. Lian ML, Chakrbarty D, Paek KY (2002) Effect of plant growth regulators and medium composition on cell growth and saponin production during cell suspension culture of mountain ginseng (Panax ginseng C. A. Mayer). J Plant Bioi 45: 201-206 
    5. Wang HO, Zhong, JJ, Yu JT (1997) Enhanced production of taxol in suspension cultures of Taxus chinensis by controlling inoculum size. Biotechnol Lett 19: 353-355 
    6. Paek KY, Hahn EJ, Son SH (2001) Application of bioreactors for large-scale micropropagation systems of plants In Vitro Cell Dev. BioI. 37:284-292 
    7. Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improving ginsenoside accumulation in adventitious root culture of Panax ginseng CA Meyer. Biochem Eng J 11: 211-215 
    8. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. physiol. Plant. 15: 473-479 
    9. Ryu, Dewey DY, Lee SO (1990) Determination of growth rate for plant cell cultures: Comparative studies. Biotechnol Bioeng 35: 305-311 
    10. Ozeki Y, Komamine A (1985) Effects of inoculum density, zeatin and sucrose on anthocynin accumulation in a carrot suspension culture. Plant Cell Tiss Org Cult 5:45-53 
    11. Fujita Y, Hara Y (1985) The effective production of shikonin by cultures with an increased cell population with an increased cell population. Afric Bioi Chem 49: 2071-2075 
    12. Su WW, Lei F (1993) Rosmarinic acid production in perfused Anchusa officinalis culture: effect of inoculum size. Biotechnol Lett 15: 1035-1038 
    13. Sakurai M, Mori T, Seki M, Furusaki S (1996) Changes of anthocyanin composition by conditioned medium and cell inoculum size using strawberry suspension culture. Biotechnol Lett 18: 1149-1154 
    14. Akalezi CO, Liu S, Li OS, Yu JT, Zhong JJ (1999) Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng. Process Biochem 34: 639-642 
  • 이 논문을 인용한 문헌 (1)

    1. 2009. "" Journal of the Korean Society for Applied Biological Chemistry, 52(5): 466~471     

 저자의 다른 논문

  • 한은주 (24)

    1. 2000 "Production of Adventitious Ginseng Roots Using Biorectors" 식물조직배양학회지 = Korean journal of plant tissue culture 27 (4): 309~315    
    2. 2001 "Effects of Macro Elements and Nitrogen Source on Adventitious Root Growth and Ginsenoside Production in Ginseng (Panax ginseng C. A. Meyer)" Journal of plant biology = 식물학회지 44 (4): 179~184    
    3. 2001 "기내 배양환경이 오리엔탈 백합 '카사블랑카' 의 기외 발아에 미치는 영향" 식물조직배양학회지 = Korean journal of plant tissue culture 28 (6): 317~321    
    4. 2003 "Adventitious Root Cultures of Panax ginseng C.V. Meyer and Ginsenoside Production through Large-Scale Bioreactor System" Journal of plant biotechnology 5 (1): 1~6    
    5. 2003 "IBA와 NAA 처리에 의해 생성된 Ethylene이 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 발달에 미치는 영향" 식물생명공학회지 = Korean journal of plant biotechnology 30 (2): 173~177    
    6. 2004 "다양한 생물반응기 형태가 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 생산에 미치는 영향" 식물생명공학회지 = Korean journal of plant biotechnology 31 (3): 249~253    
    7. 2004 "생물반응기에서 배양된 마늘 신초와 Microbulb의 형태발달 및 조직관찰" 식물생명공학회지 = Korean journal of plant biotechnology 31 (4): 301~306    
    8. 2004 "Effect of Polyploidy Induction on Biomass and Ginsenoside Accumulations in Adventitious Roots of Ginseng" Journal of plant biology = 식물학회지 47 (4): 356~360    
    9. 2005 "생물반응기내의 공기주입량 및 Sparger 형태가 인삼 (Panax ginseng C.A. Meyer) 부정근의 생장과 Ginsenoside 함량에 미치는 영향" 식물생명공학회지 = Korean journal of plant biotechnology 32 (2): 111~116    
    10. 2006 "식물생장조절물질, 무기물 농도 및 질소원 비율이 Gymmma sylvestre 세포 배양에 미치는 영향" Journal of plant biotechnology = 식물생명공학회지 33 (2): 105~110    
  • Paek, Kee-Yoeup (94)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기