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Journal of fisheries science and technology v.7 no.1, 2004년, pp.10 - 15   피인용횟수: 1

Pathogenic Vibrio spp. Isolated from the Gwangan Beach of Busan in 2003

Park Mi-Yeon    (Department of Food Science and Technology, Pukyong National University   ); Park Chan-Woong    (Department of Food Science and Technology, Pukyong National University   ); Kwon Chil-Sung    (Department of Food Science and Technology, Pukyong National University   ); Chang Dong-Suck    (Department of Food Science and Technology, Pukyong National University  );
  • 초록

    A total of 52 pathogenic Vibrio strains was isolated from the Gwangan Beach during summer in 2003. The isolated vibrios were composed of 6 different species: V. parahaemolyticus, V. cholerae non O1, V. fluvialis, V. vulnificus, V. alginolyticus, and V. mimicus. V. parahaemolyticus was most predominant as $46\%$ (24/52), V. cholerae non O1 was the second with $23\%$ (12/52), and V. fluvialis was the third with $17\%$ (9/52). Among the isolated strains, 22 strains showed hemolytic, proteolytic or ureolytic activity. Eight strains showed both hemolysin and protease activities, and either 6 strains showed only hemolysin activities and 7 strains only protease activities. Only one strain of V. parahaemolyticus isolates showed urease activity. The urease-positive V. parahaemolyticus strain (V. parahaemolyticus S25) showed the same biochemical characteristics as the reference strain, V. parahaemolyticus KCTC 2471 (urease­negative) except for urease production. To compare the degree of virulence of Vibrio strains having different pathogenic factors, hemolysin, protease, or urease-positive strains were injected into groups of 10 each of ICR mice (7- to l0-week-old male). The lethal rate of urease-positive V. parahaemolyticus S25 was significantly high, being $70\%$ . Protease-positive strains showed $40-60\%$ of lethal rate. Hemolysin-positive strains showed no mortality, similar to non-pathogenic V. parahaemolyticus KCTC 2471 and V. parahaemolyticus FM12.


  • 주제어

    Pathogenic Vibrio .   Hemolysin .   Potease .   Urease.  

  • 참고문헌 (41)

    1. Abbott, S L., C. Powers, C.A. Kaysner, Y. Takeda, M. Ishibashi, S.W. Joseph and J.M. Janda. 1989. Emergence of a restricted bioserovar of Vibrio parahaemolyticus as the predominant cause of Vibrioassociated gastroenteritis on the West Coast of the United States and Mexico. J. Clin. Microbiol., 27, 2891-2893 
    2. Aronson, M., O. Medalia and B. Griffel. 1974. Prevention of ascending pyelonephritis in mice by urease inhibitors. Nephron, 12, 94-104 
    3. Braude, A. and J. Siemienski. 1960. Role of bacterial urease in experimental pyelonephritis. J. Bacteriol., 80, 171-179 
    4. Cai, YL. and Y.X. Ni. 1996. Purification, characterization, and pathogenicity of urease produced by Vibrio parahaemolyticus. J. Clin. Lab. Anal., 10, 70-73 
    5. Chang, D.S. and S. Shinoda. 1994. Toxin produced by pathogenic vibrios isolated from seafood. Bull. Kor. Fish. Soc., 27, 107-113 
    6. Cover, T.L. and M.J. Blaser. 1995. Helicobacter pylori: a bacterial cause of gastritis, peptic ulcer disease, and gastric cancer. ASM News, 60, 21-26 
    7. Dalsgaard, A., MJ. Albert, D.N. Taylor, T. Shimada, R. Meza, O. Serichantalergs and P. Echeverria. 1995. Characterization of Vibrio cholerae non O1 serogroups obtained from an outbreak of diarrhea in Lima, Peru. J. Clin. Microbiol., 33, 2715-2722 
    8. Edward, P.D., J.M. Janda, I.A Frederic and J.B. Edward, 1984. Comparative studies and laboratory diagnosis of Vibrio vulnificus, an invasive Vibrio sp. J. Clin. Microbiol., 19, 122-125 
    9. Fujino, T., R. Sakazakim and K. Tamura. 1974. Designation of types strain of Vibrio parahaemolyticus and description of 200 strains of the species. Int. J. Syst. Bacteriol., 24, 447-449 
    10. Gray, L.D. and A.S. Kreger. 1985. Purification and characterization of an extracellular cytolysin produced by Vibrio vulnificus. Infect. Immun., 48, 62-72 
    11. Hawtin, P.R., A.R. Stacey and D.G. Newell. 1990. Investigation of the structure and localization of the urease of Helicobacter pylori using monoclonal antibodies. J. Gen. Micobiol., 136, 1995-2000 
    12. Honda, S., S. Matsumoto, T. Miwatani and T. Honda. 1992. A survey of urease-positive Vibrio paralwemolyticus strains isolated from traveller's diarrhea, seawater and imported frozen seafoods. Eur. J. Epidemol., 8, 861-864 
    13. Honda, T., M. Arita, T. Takeda, M. Yoh and T Miwatani. 1985. Non O1 Vibrio cholerae produces two newly identified of toxins related to Vibrio parahaemolyticus hemolysin and Escherichia coli heat-stable enterotoxin. Lancet, II, 163-164 
    14. Honda, T., Y. Ni and T. Miwatani. 1988. Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin. Infect. Immun., 56, 961-965 
    15. Huq, M.I., D. Huber and G. Kibryia. 1979. Isolation of urease producing Vibrio parahaemolyticus strains from cases of gastroenteritis. Indian J. Med. Res., 70, 549-553 
    16. lchinose, Y., K. Yamamoto, N. Nakasone, M.J. Tanabe, T. Takeda, T. Miwatani and M. Iwanaga. 1987. Enterotoxicity of EI Tor like hemolysin of Vibrio cholerae non O1. Infect. Immun., 55, 1090-1093 
    17. Johnson, D.E., R.G. Russell, C.V. Lockatell, J.W. Warren and H.L.T. Mobley. 1993. Contribution of Proteus mirabilis urease to persistence, urolithiasis, and acute pyelonephritis in a mouse model of ascending urinary tract infection. Infect. Immun., 61, 2748-2754 
    18. Jones, B.D., C.V. Lockatell, D.E. Johnson, J.W. Warren and H.L.T. Mobley. 1990. Construction of a ureasenegative mutant of Proteus mirabilis: analysis of virulence in a mouse model of ascending urinary tract infection. Infect. Immun., 58, 1120-1123 
    19. Kaysner, C.A., C. Abeyta, P.A. Trost, W.E. Hill and M.M. Wekell. 1994. Urea hydrolysis can predict the potential pathogenicity of Vibrio parahaemolyticus strains isolated in the Pacific Northwest. Appl. Environ. Microbiol., 60, 3020-3022 
    20. Kelly, M. and E.M.D. Stroh. 1989. Urease positive, Kanagawa negative Vibrio parahaemolyticus from patients and the environment in the Pacific Northwest. J. Clin. Microbiol., 27, 2820-2822 
    21. Kim, J.S. 1999. Characteristics of urease produced by Vibrio parahaemolyticus. Ph.D. Thesis, Dongeui University, Busan, Korea, pp. 18-42 
    22. Kim, S.H., M.Y., Park, Y.E. Lee, M.H. Cho and D.S. Chang. 1997. Characteristics of hemolysin produced by Vibrio cholerae non O1 FM-3 isolated from seawater. J. Kor. Fish. Soc., 30, 556-561 
    23. Kim, Y.M., B.H. Lee, S.H. Lee and T.S. Lee. 1990. Distribution of Vibrio vulnificus in seawater of Kwangan Beach Busan, Korea. Bull. Kor. Fish. Soc., 22, 385-390 
    24. Kim, Y.M., I.S. Shin and D.S. Chang. 1987. Distribution of Vibrio vulnificus the coast of South Korea. Bull. Kor. Fish. Soc., 20, 591-600 
    25. Kosary, M.K. and A.S. Kreger. 1985. Production and partial characterization of an elastolytic protease of Vibrio vulnificus. Infect. Immun., 50, 534-540 
    26. Larsen, J.L. 1984. Vibrio angillarum: Infection of temperature, pH, NaCI concentration and incubation time on growth. Appl. Bacteriol., 57, 237-246 
    27. Lee, W.J. and W.K. Choi. 1973. Vibrio parahaemolyticus in Korean coastal waters 3. The distribution of vibrio flora in the public beach of Busan city. Bull. Kor. Fish. Soc., 6, 20-26 
    28. Magalhaes, M., Y. Takeda, V. Magalhaes and S. Tateno. 1992. Brazilian urease-positive strains of Vibrio parahaemolyticus carry genetic potential to produce the TDH-related hemolysin. Mem. Inst. Oswaldo Cruz, 87, 167-168 
    29. Miyamoto, Y., T. Kato, Y. Obara, S. Akiyama, K. Takizawa and S. Yamai. 1969. In vitro hemolytic characteristics of Vibrio parahaemolyticus: its close correlation with human pathogenicity. J. Bacteriol., 100, 1147-1149 
    30. Musher, D.M., D.P. Griffith, D. Yawn and R.D. Rossen. 1975. Role of urease in pyelonephritis resulting from urinary tract infection with Proteus. J. Infect. Dis., 131, 177-181 
    31. Nolan, C.M., J. Ballard, C.A. Kaysner, J.L. Lilja, L.P. Williams and F.C. Tenover. 1984. Vibrio parahaemolyticus gastroenteritis: an outbreak associated with raw oysters in the Pacific Northwest. Diagn. Microbiol. Infect. Dis., 2, 119-128 
    32. Norqvist, A., B. Norrman, and H.W. Watz. 1990. Identification and characterization of a zinc metalloprotease associated with invasion by the fish pathogen Vibrio anguillarum. Infect. Immun., 58, 3731-3736 
    33. Oberhofer, T.R. and J.K. Podgore. 1982. Urea-hydrolyzing Vibrio parahaemolyticus associated with acute gastroenteritis. J. Clin. Microbiol., 16, 581-583 
    34. Pal, A., T. Ramamurthy, R.K. Bhadra, T. Takeda, T. Shimada, Y. Takeda, G.B. Nair, S.C. Pal and S. Chakrabarti. 1992. Reassessment of the prevalence of heat-stable enterotoxin (NAG-ST) among environmental Vibrio cholerae non O1 strains isolated from Calcutta, India, by using a NAG-ST DNA probe. Appl. Environ. Microbiol., 58, 2485-2489 
    35. Park, M.Y., H.J. Kim and D.S. Chang. 2003. Pathogenic Vibrio spp. isolated from the Gwangan Beach of Busar, 2002. J. Fish. Sci. Tech., 6, 105-109 
    36. Park, M.Y., H.J. Kim, S.T. Choi, E.K. Oh and D.S. Chang. 2002. Pathogenic factors of Vibrio spp. isolated from seawater of Gwangan Beach in Busan. J. Fish. Sci. Tech.. 5, 178-182     
    37. Russel, R.G., B.D. Tall and J.G. Morris. 1992. Non O1 Vibrio cholerae intestinal pathology and invasion in the removable intestinal tie adult rabbit diarrhea model. Infect. Immun., 60, 435-442 
    38. Saha, P.K, H. Koley and G.B. Nair. 1996. Purification and characterization of an extracellular secretogenic non-nembrane-damaging cytotoxin produced by clinical strains of Vibrio cholerae non O1. Infect. Immun., 64, 3101 -3 I08 
    39. Seong, H.K. 1997. Studies on the classificatory characteristics and virulence factors of Vibiro cholerae non O1. Ph.D. Thesis, Pukyong Natl Univ., Busan, Korea, pp. 27-43 
    40. Starks, A.M., T.S. Schoeb, M.L. Tamplin, S. Parven, T.J. Doyle, P.E. Bomeisl, G.M. Escudero and P.A. Gulig. 2000. Pathogenesis of infection by clinical and environmental strains of Vibrio vulnificus in irondextran-treated mice. Infect. Immun., 68, 5785-5793 
    41. Suthienkul, O., M, Ishibashi, T. Iida, N. Nettip, S. Supave J.B. Eampokalap, M. Makino and T. Honda. 1995. Urease production correlates with possession of the trh gene in Vibrio parahaemolyticus strains isolated in Thailand. J. Infect. Dis., 172, 1405-1408 
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