본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

느타리버섯 재배사에서 한국산 Steinernema와 Heterorhabditis를 이용한 버섯혹파리(Mycophila speyeri)의 생물적 방제
Biological Control of Mycophila speyeri Barnes (Diptera: Cecidomyiidae) using Korean Steinernema and Heterorhabditis Isolates in Pleurotus ostreatus Cultivation House

김형환   (원예연구소 원예환경과CC0072673  ); 추호렬   (경상대학교 농업생명과학대학 응용생물환경학과, 환경생명과학국가핵심연구센타UU0000114  ); 이동운   (상주대학교 농업과학연구소UU0000665  ); 이흥수   (경남농업기술원 식물환경과  ); 전흥용   (원예연구소 원예환경과CC0072673  ); 하판정   (주식회사 세실  );
  • 초록

    느타리버섯 재배사에서 버섯혹파리(Mycophila speyeri)에 대한 한국산 곤충병원성 선충 Steinernema carpocapsae 포천 계통, S. logicaudum 논산 계통, S. glaseri 동래 계통, Heterorhabditis bacteriophora 함양 계통과 Heterorabditis sp. 경산 계통의 방제효과를 평가하였다. 5종의 한국산곤충병원성 선충 중 버섯혹파리에 대한 방제는 steinernema 속 3종보다 Heterorhabditis 속 2종의 효과가 높았다. 방제효과가 가장 높았던 종은 H. bacteriophora 함양 계통이었으며, $1.5 m^2$ 당 $1\times10^6$ 마리로 처리한 7일, 14일, 21일째 방제효과는 창녕에서 $49.0\%,\;89.5\%,\;89.1\%$ 였고, $1\times10^5$ 마리로 처리했을 때는 $46.5\%,\;76.7\%,\;85.4\%$ 였다. 그리고 진주에서는 $1\times10^6$ 마리로 처리한 7일, 14일, 21일째 방제효과가 $54.0\%,\;74.5\%,\;79.8\%$ 였으며, $1\times10^5$ 마리로 처리했을 때는 $49.0\%,\;76.6\%,\;61.1\%$ 였다.


    The potential of five entomopathogenic nematodes, Steinernema carpocapsae Pocheon strain, S. logicaudum Nonsan strain, S. glaseri Dongrae strain, Heterorhabditis bacteriophora Hamnyang strain, and Heterorabditis sp. Gyeongsan strain were evaluated as biological control agents against a mushroom fly, Mycophila speyeri in the mushroom, Pleurotus ostreatus cultivation house. Control effect of M. speyeri was significantly different according to nematode species. Heterorhabditis was more effective than Steinernema. H. bacteriophora Hamnyang strain showed the highest control effect representing $49.0\%$ (7 days), $89.5\%$ (14 days) and $89.1\%$ (21 days post-treatment) at the rate of $1\times10^6$ and $46.5\%$ (7 days), $76.6\%$ (14 days) and $85.4\%$ (21 days post-treatment) at the rate of $1.0\times10^5$ Ijs/ $1.5 m^2$ in Changnyoung, Gyeongnam, respectively. In Jinju, Gyeongnam, control effects of the sa me species were $54.0\%$ (7 days), $74.5\%$ (14 days), and $79.8\%$ (21 days post-treatment) at the rate of $1\times10^6$ and $49.0\%$ (7 days), $76.6\%$ (14 days), and $61.1\%$ (21 days post-treatment) at the rate of $1.0\times10^5$ Ijs/ $1.5 m^2$ , respectively.


  • 주제어

    버섯혹파리 .   느타리버섯 .   생물적 방제.  

  • 참고문헌 (41)

    1. Chung, S.L. and R. Snetsinger. 1968. Comparative effects of certain environmental factors upon the life cycles of two species of mushroom infesting cecid flies. Mushroom Science VII: 247-256 
    2. Dutky, S.R., J.V. Thompson and G.E. Cantwell. 1964. A technique for the mass propagation of the DD-136 nematode. J. Insect Pathol. 6: 417-422 
    3. Gouge, D.H. and N.G.M. Hague. 1995. The susceptibility of different species of sciarid flies to entomopathogemc nematodes. J. Helminthol. 69: 313-318 
    4. Grewal, P.S., P.N. Richardson, G. Collins and R.N. Edmondson. 1992. Comparative effects of Steinernema feltiae (Nematoda: Steinemematidae) and insecticides on yield and cropping of the mushroom Agaricus bisporus. Ann. Appl. Biol. 121: 511-520 
    5. Han, Y.S., G.C. Shin and G.P. Kim. 1977. An expenment on the protection against mushroom sporophore contamination caused by a mushroom-infesting cecid fly, Mycophlla sp. (Dlptera: Cecidomymyiidae). Res. Rept. RDA. 19: 21-25 
    6. Jhune, C.S., C.H. You, D.Y. Cha and G.P. Kim. 1990. Selection and applying method of pesticides for control of mushroom flies during the cultivation of oyster mushroom, Pleurotus spp.. Res. Rept. RDA. 2: 64-70 
    7. Kim, H.H, H.Y. Choo, D.W. Lee, S.M. Lee, H.Y. Jeon, M.R. Cho and M.S. Yiem. 2003. Control efficacy of Korean entomopathogenic nematodes against fungus knat, Bradysia agrestis (Diptera: Sciaridae) and persistence in bed soil. J. Kor. Soc. Hort. Sci. 44: 393-401 
    8. Kim, S.R., K.H. Choi, E.S. Choi, W.J. Yang, B.R. Jin and H.D. Sohn. 1999. An investigation of the major dipteran pests on the oyster mushroom (Pluerotus ostreatus) in Korea. Koran J. Appl. Entomol. 38: 41-46 
    9. Lee, H.S. 2002. Studies on the biology and damage of Lycoriella mali (Diptera: Sciaridae) as pest of oyster mushroom in Korea. PhD thesis of Chonnam National University 79pp 
    10. Lee, H.S., H.W. Lee, W.G. Shin and C.G. Park. 1998. Studies of control of pests and insect pests in mushroom. Report of Kyongnam ARES. 239-246 
    11. Lee, H.S., K.J. Kim and H.U. Lee. 1998. Effect of temperature on the development of sciarid fly, Bradysia sp. (Diptera: Sciaridae). Korean J. Appl. Entomol. 37: 171-178 
    12. Ministry of Agriculture and Forestry Republic of Korea. 2002. Agricultural & forestry statistical yearbook. pp.300-301 
    13. Sanui, T. and J. Yukawa. 1986. Discovery of a paedogenetic gall midge (Diptera: Cecidomyiidae) in Japan and notes on its reproduction in vitro and infestation in a cultivating factory of the oyster mushroom. Jpn. J. Appl. Entomol. Zool. 30: 50-54 
    14. Bedding, R. and A. Molyneux. 1982. Penetration of insect cuticle by infective juveniles of Heterorhabditis spp. (Heterorhabdltidae: Nematoda). Nematologica 28: 354-359 
    15. Duso, C. and E. Vettorazzo. 1991. Esperienze di lotta contro Bradysia paupera Tuom. (Diptera, Sciaridae) su carciofo e begonia in coltura protetta. Motiziario Sullemalattie Deffle Piante. 112: 89-99 
    16. Kaya, H.K. and S.P. Stock. 1997. Technique in insect pathology, pp.281-324. In: L. A. Lacey (eds.). Manual of techniques in insect pathology. Academic Press, San Diego, CA, USA 
    17. Lee, H.S., K.C. Kim and B.K. Chung. 2001. A report on Megaselia tamiladuensis Disney (Diptera: Phoridae) as a pest of oyster mushroom, Pleurotus ostreatus in Korea. Korean J. Appl. Entomol. 40: 345-348 
    18. Adams, B. and K.B. Nguyen. 2002. Taxonomy and systematics. pp. 1-34. In Entomopathogenic nematology, ed. by R. Gaugler. 388 pp. CABI publishing, Oxon 
    19. Mr$\'a$$\v{c}$ek, Z., S. Be$\v{c}$v$\'a$$\v{r}$, P. Kindlmann and Z.M. Webster. 1999. Factors influencing the infectivity of a Canadian isolate of Steinernema kraussei (Nematoda: Steinernematidae) at low temperature. J. Invertebr. Pathol. 73: 243-247 
    20. Scheepmaker, J.W.A., F.P. Geels, P.H. Smits and L.J.L.D. van Griensven. 1997. Control of the mushroom pests Lycoriella auripila (Diptera: Sciaridae) and Megaselia halterata (Diptera: Phoridae) by Steinernema feltiae (Nematoda: Steinernematidae) in field experiments. Ann. Appl. Biol. 131: 359-368 
    21. Yukawa, J. 1996. Identification of paedogenetic gall midge, Mycophila speyeri (Diptera: Cecidomyiidae) and possibility of accidental introduction to Japan. Jpn. J. Appl. Entomol. Zool. 40: 135-143 
    22. Lee, H.S., K.J. Kim, G.W. Song and J.H. Kim. 1999b. Isolation method of mushroom infesting pests from mushroom growmg compost. Korean J. Mycol. 27: 289-292 
    23. Lee, H.S. and K.J. Kim. 2003. Report on Mycophila speyeri Bames (Diptera: Cecidomyiidae) as a pest of mushroom cultivation in Korea. Korean J. Appl. Entomol. 42: 71-75 
    24. Clift, A.D. 1979. The identity, economic importance and control of insect pests of mushrooms in new south wales, Australia. Mushroom Science X: 367-383 
    25. Kim, H.H., H.Y. Choo, H.S. Lee, C.G. Park, D.W. Lee, B.R. Jin and Y.M. Choo. 2001. Biological control of Lycoriella mali (Diptera: Sciaridae), a pest of oyster mushroom, Pleurotus ostreatus using entomopathogenic nematodes. Korean J. Appl. Entomol. 40: 59-67 
    26. Park, Y.J., G.H. Kim and Y.G. Kim. 2003. ComparatIve analysis of host insect immunodepression induced by two entomopathogenic bacteria, Xenorhabdus nematopilus and Staphylococcus gallinarum, with different pathogenicities. Korean J. Appl. Entomol. 42: 353-360 
    27. Lee, H.S., K.C. Kim, C.G. Park and W.K. Shin. 1999a. Description of fungus gnat, Lycoriella mali Fitch (Diptera: Sciaridae) from Korea. Korean J. Appl. Entomol. 38: 209-212 
    28. Park, C.G., J. Yoo, M. Sasakawa, H.Y. Choo, H.H. Kim and H.S. Lee. 1999. Notes on newly recorded insect pest, Bradysia agrestis (Diptera: Sciaridae). Korean J. Appl. Entomol. 38: 59-62 
    29. Richardson, P.N. and P.S. Grewal. 1991. Comparative assessment of biological (Nematoda: Steinernema feltiae) and chemical methods of control for the mushroom fly Lycoriella auripila (Diptera: Sciaridae). biocont. Sci. Technol. 1: 217-228 
    30. White, P.F. 1990. Effects of the paedogenetic mushroom cecid, Heteropeza pygmaea (Diptera: Cecidomyiidae) on cropping of the cultivated mushroom (Agaricus bisporus). Ann. Appl. Biol. 117: 63-72 
    31. Woodring, J.L. and H.K. Kaya. 1988. Steinernematidae and heterorhabditid nematodes: A handbook of techniques. Southern Coop. Ser. Bull. 331, Alkansas Agri. Exp. Stn. Fayetteville, AR. 29pp 
    32. Chung, S.L. and R. Snetsinger. 1965. Environmental effects upon reproduction of.a mushroom-infesting cecid fly, Mcophila speyeri (Barnes) (Diptera: Cecidomyiidae). Cand. Ent. 97: 1318-1323 
    33. Gouge, D.H. and N.G.M. Hague. 1994. Control of sciarids in glass and propagation houses, with Steinernema feltiae. Brighton Crop Protec. Conf. 1073-1078 
    34. Grewal, P.S., M. Tomalak, C.B.O. Keil and R. Gaugler. 1993. Evaluation of a genetically selected strain of Steinernema feltiae against the mushroom sciarid Lycoriella mali. Ann. Appl. Biol. 123: 695-702 
    35. Thomson, W.T. 1992. A worldwide guide to beneficial animals used for pest control purposes. 92 pp. Thomson Publications. CA. USA 
    36. Kang, YJ., D.W. Lee, H.Y. Choo, S.M. Lee, T.W. Kweon and S.M. Lee. 2004. Biological control of Spodoptera depravata (Butler) (Lepidoptera: Noctuidae) using entomopathogenic nematodes. Korean J. Appl. Entomol. 43: 61-70 
    37. Tomalak, M. and J.J. Lipa. 1991. Factors affecting entomophilic activity of Neoaplectana feltiae in mushroom compost. Entomol. expo Appl. 59: 105-110 
    38. Kim, H.H, H.Y. Choo, H.K. Kaya, D.W. Lee, S.M. Lee and H.Y. Jeon. 2004. Steinernema carpocapsae (Rhabditida: Steinernematidae) as a biological control agent against the fungus gnat Bradysia agrestis (Diptera: Sciaridae) in propagation houses. Biocont. Sci. Technol. 14: 171-183 
    39. Pritchard, A.E. 1960. A new classification of the paedogenic gall midges formerly assigned to the subfamily Heteropezinae (Diptera; Cecidomyiidae). Ann. Ent. Soc. Amer. 53: 305-316 
    40. SAS Institute. 1996. 'SAS 6.11 for Window' SAS Institute, Cary, NC, USA 
    41. Richardson, P.N. and J.C. Hushes. 1986. Use of the nematode Heterorhabditis heliothidis to control mushroom cecidomyiid flies (Diptera: Cecidomyiidae). Abstracts X VIII International Nematology Symposium, Antibes, September, p.25 
  • 이 논문을 인용한 문헌 (1)

    1. Yoon, Jung-Beom ; Kim, Hyeong-Hwan ; Jung, Chung-Ryul ; Kang, Min-Gu ; Kwon, Sun-Jung ; Kim, Dong-Hwan ; Yang, Chang-Yeol ; Seo, Mi-Hye 2016. "Molecular Identification of the Dominant Species of Dark-winged Fungus Gnat (Diptera: Sciaridae) from Button Mushroom (Agaricus bisporus) in Korea" 한국응용곤충학회지 = Korean journal of applied entomology, 55(4): 471~475     

 저자의 다른 논문

  • 추호렬 (123)

    1. 1979 "제주도의 식물기생성 선충에 관하여" 한국식물보호학회지 18 (4): 169~175    
    2. 1981 "수입재해충 나무좀류의 분류 I. 나무좀과와 긴나무좀과" 한국식물보호학회지 20 (4): 196~206    
    3. 1983 "과수 및 원예식물을 가해하는 나무좀류 목록" 한국식물보호학회지 22 (3): 171~173    
    4. 1983 "한국산 나무좀과 해충의 미기록종에 관하여" 한국식물보호학회지 22 (3): 174~180    
    5. 1983 "수입재해충 나무좀류의 분류 II 개나무좀과" 한국식물보호학회지 22 (1): 30~34    
    6. 1983 "수입재해충 나무좀류의 분류 III 나무좀과와 긴나무좀과" 한국식물보호학회지 22 (1): 34~41    
    7. 1985 "양다래 기생(寄生) 뿌리혹선충의 발견(發見)" 한국식물보호학회지 24 (2): 115~115    
    8. 1985 "한국산(韓國産) 나무좀과(科) 및 긴나무좀과(科) 해충(害蟲)과 기주식물(寄主植物) 목록(目錄)" 한국식물보호학회지 24 (3): 163~167    
    9. 1987 "복숭아와 자두꽃을 방화(訪花)하는 곤충(昆蟲)의 종류(種類)와 생태(生態)" 한국식물보호학회지 26 (3): 117~122    
    10. 1987 "산림토양의 절지 동물상에 관한 연구" 한국식물보호학회지 26 (3): 133~138    
  • 전흥용 (22)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
  • 한국응용곤충학회 : 저널
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기