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Biotechnology and bioprocess engineering v.9 no.6, 2004년, pp.423 - 427   피인용횟수: 2

A Simple Purification Procedure of Biologically Active Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF) Secreted in Rice Cell Suspension Culture

Sharma Niti    (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University   ); Park Seung Moon    (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University   ); Kwon Tae Ho    (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University   ); Kim Dae Hyuk    (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University   ); Yang Moon Sik    (Institute for Molecular Biology and Genetics, Basic Science Research Institute, Chonbuk National University  );
  • 초록

    A simple purification procedure of bioactive human granulocyte macrophage colony stimulating factor (hGM-CSF) secreted in rice cell suspension culture has previously been described. In this study the protein was purified to apparent homogeneity with an overall yield of $80.1\%$ by ammonium sulfate precipitation and a single chromatographic step involving FPLCanion exchange chromatography. The purified hGM-CSF revealed at least five glycosylated forms ranging from $21.5{\~}29$ kDa, and its biological activity was independent of the glycosylation pattern. This is the first purification report of recombinant hGM-CSF to apparent homogeneity from rice cell suspension cultures.


  • 주제어

    hGM-CSF .   rice cell suspension culture .   protein purification .   glycosylation.  

  • 참고문헌 (25)

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  • 이 논문을 인용한 문헌 (2)

    1. 2005. "" Biotechnology and bioprocess engineering, 10(3): 242~247     
    2. 2007. "" Biotechnology and bioprocess engineering, 12(6): 594~600     

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