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Biotechnology and bioprocess engineering v.9 no.6, 2004년, pp.459 - 464   피인용횟수: 1

The Improvement of Cephalosporin C Production by Fed-batch Culture of Cephalosporium acremonium M25 Using Rice Oil

Kim Jin Hee    (Department of Chemical and Biological Engineering, Korea University   ); Lim Jung Soo    (Department of Chemical and Biological Engineering, Korea University   ); Kim Seung Wook    (Department of Chemical and Biological Engineering, Korea University  );
  • 초록

    The objective of this study is to improve cephalosporin C (CPC) production byoptimization of medium and culture conditions. A statistical method was introduced to optimize the main culture medium. The main medium for CPC production was optimized using a statistical method. Glucose and corn steep liquor (CSL) were found to be the most effective factors for CPC production. Glucose and CSL were optimized to 2.84 and $6.68\%$ , respectively. CPC produc­tion was improved $50\%$ by feeding of $5\%$ rice oil at day 3rd and 5th day during the shake flask culture of C acremonium M25. The effect of agitation speeds on CPC production in a 2.5-L bio­reactor was also investigated with fed-batch mode. The maximum cell mass (54.5 g/L) was obtained at 600 rpm. However, the maximum CPC production (0.98 g/L) was obtained at 500 rpm. At this condition, the maximum CPC production was improved about $132\%$ compared to the re­sult with batch flask culture.


  • 주제어

    cephalosporin C .   Cephalosporium acremonium .   optimization .   statistical method .   rice oil.  

  • 참고문헌 (17)

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  • 이 논문을 인용한 문헌 (1)

    1. 2010. "" Journal of microbiology and biotechnology, 20(3): 480~484     

 저자의 다른 논문

  • Kim Jin Hee (1)

    1. 2002 "Morphological and rheological properties of culture broth of Cephalosporium acremonium M25" Korea-Australia rheology journal 14 (1): 11~16    
  • Kim, Seung-Wook (64)

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