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Quantification of Bacillus Species in a Wastewater Treatment System by the Molecular Analyses

Mori Koji    (Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE)   ); Iriye Ryozo    (Departmentof Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University   ); Hirata Mutsunori    (Department of Bioprocessing, Faculty of Agriculture, Gifu University   ); Takamizawa Kazuhiro    (Department of Bioprocessing, Faculty of Agriculture, Gifu University  );
  • 초록

    Bacillus species were observed and quantified by molecular approaches, using the 16S rDNA primers/probes, in a wastewater treatment plant designed for the purpose of stimulating the growth of Bacillus species. The plant has been operating as a test plant since 1997 in the city of Ina, Japan, with excellent treatment performance. Observations by in situ hybridization, using Bacillus-specific probes, indicated that Bacillus strains were inhabited in the plant and their num­bers decreased during the treatment process. Similar results were obtained from a quantitative PCR analysis using a Bacillus-specific primer set, and the amount of DNA originating from various Bacillus species was maximally $1.91%\$ of the total DNA in the wastewater treatment tank. Clone library analysis using the Bacillus-specific primers suggested that, while the population was no­ticeably increased, the phylogenetic diversity of the increasing Bacillus species was very low.


  • 주제어

    Bacillus .   wastewater treatment .   fluorescence in situ hybridization (FISH) .   quantitative PCR.  

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