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Theoretical Analysis of Chromatographic Peak Asymmetry and Sharpness by the Moment Method Using Two Peptides

Choi Du Young    (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University   ); Row Kyung Ho    (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University  );
  • 초록

    The analyses of peak shapes in chromatography are useful in operating chroma­tographic system. The asymmetry and sharpness of a chromatographic peak are estimated by the reversed-phase adsorption of two standard peptides (angiotensin II bradykinin) on $C_{18}$ In this work, the average particle diameters of $C_{18}$ were 5 and 15 $\mu$ m, while the pore sizes were 100 and 300 A. The composition of the mobile phase was $50/50\;vol.\;{\%}$ of a binary mixture of acetonitrile and water with $0.1\%$ TFA, and the particles were packed in a stainless column ( $4.6{\times}150$ mm). The third and the fourth central movement were calculated from the chromatographic elution curves by moment analysis. The peak asymmetry was determined by two theoretical calculations: the asymmetry factor by elution peak analysis and skewness with moment analysis. The sharpness was estimated by the fourth central moment. In this work, the most acceptable skewness was calculated when the pore size was 300 A. The large excess was observed on small pore size.


  • 주제어

    packings .   moment analysis .   peptides .   asymmetric factor .   skewness .   excess.  

  • 참고문헌 (11)

    1. Row, K. H., C. H. Lee, and J. H. Kang (2002) Parameters estimation of perillyl alcohol in RP-HPLC by moment analysis. Biotechnol. Bioprocess Eng. 7: 16-20     
    2. Miyabe, K. and M. Suzuki (1994) Mass-transfer phenom ena on the surface of adsorbent in reversed-phase chroma tography. Ind. Eng. Chem. Res. 33: 1792-1802 
    3. Miyabe, K. and M. Suzuki (1995) Chromatographic study on liquid-phase adsorption on octadecylsilyl-silica gel. AIChE J. 41: 548-558 
    4. Gebauer, K. H., X. L. Luo, N. G. Barton, and A. N. Stokes (2003) Efficiency of preparative and process column dis-tribution systems. J. Chromatogr. A 1006: 45-60 
    5. Lee, J. W. and K. H. Row (2001) New retention mecha-nism of mononucleotides with buffer concentrations inion-suppressing RP-HPLC. Biotechnol. Bioprocess Eng. 6:37-41 
    6. Pap, T. L. and Z. Papai (2001) Application of a new mathematical function for describing chromatographic peaks. J. Chromatogr. A 950: 53-60 
    7. Papai, Zs. and T. L. Pap (2002) Analysis of peak asymme-try in chromatography. J. Chromatogr. A 953: 31-38 
    8. Miyabe, K. and M. Suzuki (1992) Chromatography of liq-uid-phase adsorption on octadecylsily-silica gel. AIChE J.38: 901-910 
    9. Abdel, S. S. (1981) Theory and Mathematics of Chroma-tography. Verlag GmbH, Heidelberg, Germany 
    10. Kanji, M., S. Sayuri, and G. Georges (2001) Retention and mass transfer characteristics in reversed-phase liquid chromatography using a tetrahydrofuran-water solution as the mobile phase. J. Chromatogr. A 919: 231-244 
    11. Lee, S. K. and K. H. Row (2004) Optimum separation condition of peptides in reversed-phase liquid Chromatog-raphy. J. Chromatogr. B 800: 115-120 

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