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Biotechnology and bioprocess engineering v.9 no.6, 2004년, pp.500 - 505   피인용횟수: 2

Purification During Crossflow Electromicrofiltration of Fermentation Broth

Park Young G.    (Prundle Envitech. LTD, Departmet of Chemical Engineering, Daejin University  );
  • 초록

    The present study was to investigate the purification of a fermentation broth by an electromicrofiltration membrane. Microfiltration runs with a crude and a centrifuged broth, with solution of particles recovered from centrifugation and with permeates from microfiltration experiments were thus compared. Microfiltration performances were governed by colloids and small particles that induced sharp initial flux declines. For these results, the evolution of the overall membrane resistance was increased by $80\%$ in comparison with the electromicrofiltration membrane. The main focus of this study was set on the enhancement of the filtrate flux by an electric field. This pressure electrofiltration leads to a drastic improvement of the filtration by $100\%$ and the filtration time was thereby reduced. Pressure electrofiltration serves as an inter­esting alternative to the cross-flow filtration and it effectively separates advantageous constitu­ents such as amino acids and biopolymers from a fermentation broth. They were equally main­tained during the microelectrofiltration, although they were significantly reduced by $45\%$ by the microfiltration without the application of an electric field. Accordingly, since the electrofiltration membrane was provided more permeability, this study experimentally demonstrates that the permeability inside a membrane can be controlled using an electric field.


  • 주제어

    fermentation .   microfiltration .   broth .   centrifugation .   electric field .   electrofiltration.  

  • 참고문헌 (12)

    1. Engler, J. and M. Wiesner (1999) Particle fouling of a rotating membrane disk. Wat. Res. 34: 557-565 
    2. Serra, C., M. Wiesner, and J. Laine (1999) Rotating membrane disk filters: Design evaluation using computational fluid dynamics. Chem. Eng. Sci. 72: 1-17 
    3. Holeschovsky, U. and C. Cooney (1991) Quantitative description of ultrafiltration in a rotating filtration device. AIChE J. 37: 1219-1226 
    4. Byers, C. and A. Amarnath (1995) Understand the potential of electro-separation. Chem. Eng. Progress Feburary: 63-69 
    5. Park, Y. (2000) Separation of $\Phi$X Hae III DNA with electrochromatography. Biotechnol. Bioprocess Eng. 5: 332-338 
    6. Lararova, Z. and W. Serro (2002) Electromembrane separation of mineral suspensions: Influence of process parameters. Sep. Sci. Tech. 37: 515-534 
    7. Galier, S. and H. Balmann (2001) Study of the mass transfer phenomena involved in an electrophoretic membrane contactor. J. Mem. Sci. 194: 117-133 
    8. Park, Y. (2002) The effect of porosity of sieving particles on the removal efficiency of organic substances via biofilter in the fixed bed. Biotechnol. Bioprocess Eng. 7: 31-37     
    9. Park, Y. (2003) Residence time distribution in the chro-matographic column: Applications in the separation engi-neering of DNA. Biotechnol. Bioprocess Eng. 8: 117-125     
    10. Krstic, D., S. Markov, and M. Tekic (2001) Membrane fouling during cross-flow microfiltration of polyporous squamosus fermentation broth. Biochem. Eng. J. 9: 103-109 
    11. Park, Y. and H. Byun (2002) Application of the cake-filtration theory to analyze the permeate performance in poly(vinylbenzyl chloride)-filled microfiltration membrane. J. Ind. Eng. Chem. 8: 537-545 
    12. Tanaka, T., Y. Yamagiwa, T. Nagano, M. Taniguchi, and K. Nakanishi (2001) Relationship between cake structure and membrane pore size in crossflow filtration of microbial cell suspension containing fine particles. J Chem. Eng. Japan 34: 1524-1531 
  • 이 논문을 인용한 문헌 (2)

    1. 2008. "" Biotechnology and bioprocess engineering, 13(6): 677~682     
    2. Hemavathi, A.B. ; Raghavarao, K.S.M.S. 2011. "Membrane Processing for Purification and Concentration of ${\beta}$-glycosidases from Barley (Hordeum vulgare)" Biotechnology and bioprocess engineering, 16(2): 282~290     

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