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Journal of microbiology and biotechnology v.14 no.6, 2004년, pp.1150 - 1156   피인용횟수: 10
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Detection of Genetically Modified Maize by Multiplex PCR Method

허문석    (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University   ); 김재환    (Institute of Life Sciences and Resources and Department of Food Science and Biotechnology, Kyung Hee University   ); 박선희    (Division of Food Microbiology, Korea Food and Drug Administration   ); 우근조    (Division of Food Microbiology, Korea Food and Drug Administration   ); 김혜용    (Division of Food Microbiology, Korea Food and Drug Administration  );
  • 초록

    The GMO (Genetically Modified Organism) labeling system on raw materials has been in Korea since March 2001, and genetically modified organisms (GMOs)-derived foods since July 2001. Therefore, we designed a multiplex PCR method to ascertain the validity of the labeling system and to monitor the status of circulation for genetically modified maize (GM Maize). Five lines of GM Maize (GA21, TC1507, Mon810, NK603, and Bt176) were used, and specific primer pairs were designed to detect each line. Using this method, the different lines of GM Maize were monitored from raw products and processed foods in Korean market. Some of the maize processed foods and raw materials were shown to contain more than one foreign gene. This method was found to be effective for-detecting five different GM Maize in a single reaction.


  • 주제어

    Genetically modified maize .   multiplex PCR.  

  • 참고문헌 (23)

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