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Journal of microbiology and biotechnology v.14 no.6, 2004년, pp.1157 - 1162   피인용횟수: 2
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Responses of Chloramphenicol Immunosensor to Analyte Types

PARK , IN-SEON    (Food Function Research Division, Korea Food Research Institute   ); KIM, DONG-KYUNG    (Food Function Research Division, Korea Food Research Institute   ); KIM, NAM-SOO    (Food Function Research Division, Korea Food Research Institute  );
  • 초록

    A well-holder type piezoelectric chloramphenicol (CAP) immunosensor which was prepared by binding an anti­CAP antibody to the chemisorbed monolayers of various thiol or sulfide compounds over the gold electrode surface of quartz crystals through a carboxyl-amine coupling procedure, using the activation with l-ethyl- 3-(3-dimethylarninopropyl)carbodiimide­HCl and N-hydroxysulfosuccinimide, was determined for its responses to CAP, CAP succinate, and water-soluble CAP. The reaction phase used in the well holder was 0.01 M phosphate buffer (pH 7.4), and the solvent for analyte dissolution varied according to the solubility of the individual analyte. The analyte detection which was indicated by a steady-state frequency shift was finished within 10 min, except for CAP dissolved in methanol. The responses of CAP succinate and water-soluble CAP in the reaction phase were very stable, while a minute fluctuation was found with CAP.


  • 주제어

    Responses .   chloramphenicol immunosensor .   analyte types.  

  • 참고문헌 (17)

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  • 이 논문을 인용한 문헌 (2)

    1. 2006. "" Journal of microbiology and biotechnology, 16(10): 1523~1528     
    2. 2006. "" Journal of microbiology and biotechnology, 16(8): 1216~1221     

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