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Journal of microbiology and biotechnology v.14 no.6, 2004년, pp.1232 - 1238   피인용횟수: 2
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Secretory Production of Rahnella aquatilis ATCC 33071 Levansucrase Expressed in Escherichia coli

KANG , SOON-AH    (Department of Molecular Biotechnology, Bio/Molecular Informatics Center, Konkuk University   ); LEE, JAE-CHEOL    (Department of Food and Nutrition, Samcheok National University   ); PARK, YOUNG-MIN    (Department of Dental Hygiene, YeoJoo Institute of Technology   ); LEE, CHAN    (Department of Food and Biotechnology, Hanseo University   ); KIM, SEUNG-HWAN    (Korea Food and Drug Administration   ); CHANG, BYUNG-IL    (RealBiotech Co. Ltd.   ); KIM, CHUL-HO    (Biotechnology Research Division, KRIBB   ); SEO, JEONG-WOO    (Biotechnology Research Division, KRIBB   ); RHEE, SANG-KI    (Biotechnology Research Division, KRIBB   ); JUNG, SUNG-JE    (Department of Food Science and Technology, Kyung Hee University   ); KIM, SANG-MOO    (Faculty of Marine Bioscience and Technology, Kangnung National University   ); PARK, SEONG-KYU    (Department of Prescriptionary, College of Oriental Medicine   ); JANG, KI-HYO    (Department of Food and Nutrition, Samcheok National University  );
  • 초록

    To investigate the production and characteristics of thermostable levan sucrase from Rahnella aquatilis ATCC 33071, the levan sucrase gene from R. aquatilis was cloned and expressed in Escherichia coli without induction system. Expression of levansucrase gene in E. coli had no notable or detrimental effect on the growth of host strain, and the recombinant levan sucrase exhibited levan synthesis activity. Levansucrase was secreted to the periplasm in E. coli, and addition of $0.5\%$ glycine yielded further secretion of levansucrase to the growth medium and resulted in an increase of total levansucrase activity. Furthermore, the cellular levansucrase was evaluated for the production of levan by using toluene­permeabilized whole-cells. The levansucrase was thermostable at $37^{\circ}C$ . The molecular size oflevan was $1{\times}\;10^{6}$ Da, as determined by HPLC, and the degree of polymerization of levan varied with incubation temperatures: Low incubation temperature was preferable for the production of high-molecular size levan. The present study demonstrated that the mass production of levan and levan oligosaccharides can be achieved by glycine supplementation to the growth medium or by toluene­permeabilized whole-cells.


  • 주제어

    Levansucrase .   functional food .   Rahnella aquatilis .   levan .   secretion.  

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    1. 2006. "" Journal of microbiology and biotechnology, 16(1): 102~108     
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