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Journal of microbiology and biotechnology v.14 no.6, 2004년, pp.1256 - 1266   피인용횟수: 1
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Channeling of Intermediates Derived from Medium-Chain Fatty Acids and De novo-SYnthesized Fatty Acids to Polyhydroxyalkanoic Acid by 2-Bromooctanoic Acid in Pseudomonas fluorescens BM07

LEE, HO-JOO    (Division of Applied Life Sciences (BK21), Graduate School   ); RHO, JONG-KOOK    (Division of Applied Life Sciences (BK21), Graduate School   ); KAMBIZ AKBARI NOGHABI,    (Division of Applied Life Sciences (BK21), Graduate School   ); LEE, SEUNG-EUN    (Division of Applied Life Sciences (BK21), Graduate School   ); CHOI, MUN-HWAN    (PMBBRC   ); YOON, SUNG-CHUL    (Division of Applied Life Sciences (BK21), Graduate School,Division of Life Science, College of Natural Sciences, PMBBRC,Environmental Biotechnology National Core Research Center, Gyeongsang National University  );
  • 초록

    2-Bromooctanoic acid (2-BrOA) is known to block the formation of polyhydroxyalkanoic acid (PHA) in Pseudomonasfluorescens BM07 without any influence on the cell growth when grown on fructose, but it inhibits the cell growth when grown on octanoate (OA) (Lee et al., Appl. Environ. Microbiol. 67: 4963- 4974, 2001). We investigated the role of 2-BrOA in the PHA synthesis of the bacterium grown with mixtures of fructose and fatty acids. OA, 11­phenoxyundecanoic acid (1 1-POU), and 5-phenylvaleric acid (5-PV) were selected as model substrates. When supplemented with 50 mM fructose, all these carboxylic acids suppressed the formation of PHA from fructose, however, the ~-oxidation coenzyme A monomers derived from the carboxylic acids were efficiently polymerized, but the conversion yield [(mol of carboxylate substrate converted into PHA)/(mol of carboxylate substrate in the feed)] was low (e.g., maximally $\~53\%$ for 5 mM 11-POU). Addition of 2-BrOA (up to 5 mM) to the mixed carbon sources raised the conversion yield sensitively and effectively only at low levels of the acid substrates (e.g., 2 mM 1 1-POU or 5 mM OA): For instance, $100\%$ of 2 mM ll-POU were converted into PHA in the presence of 5 mM 2-BrOA, whereas only $\~10\%$ of the 1 1-POU were converted in the absence of 2-BrOA. However, at highly saturated suppressing levels (e.g., 5 mM ll-POU), 2-BrOA inhibitor showed no significant additional effect on the conversion ( $60- 70\%$ conversion irrespective of 2-BrOA level). The existence of competitive and compensative relationship between 2­BrOA and all the carboxylic acid substrates used may indicate 'Present address: Section on Brain Physiology and Metabolism, Bldg. 10, Rm. 6N202, National Institute on Agmg, National Institute of Health, Bethesda, MD 20892, U.S.A. that all the acid substrate-derived inhibiting species bind to the same site as the 2-BrOA inhibiting species does. We, therefore, suggest that 2-BrOA can be used for efficiently increasing the yield of conversion of expensive substituted fatty acids into PHA and then substituted 3-hydroxyacids by hydrolyzing it.


  • 주제어

    Polyhydroxyalkanoic acid .   2-bromooctanoate .   Pseudomonas fluorescens BM07 .   monomer channeling .   de novo fatty acids.  

  • 참고문헌 (16)

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    3. Lee, H.-J., M. H. Choi, T.-U. Kim, and S. C. Yoon. 2001. Accumulation of polyhydroxyalkanoic acid containing large amounts of unsaturated monomers in Pseudomonas fluorescens BM07 utilizing saccharides and its inhibition by 2-bromooctanoic acid. Appl. Environ. Microbiol. 67: 4963- 4974 
    4. Rehm, B. H. A., N. Kroger, and A. Steinbuchel. 1998. A new metabolic link between fatty acid synthesis and polyhydroxyalkanoic acid synthesis. J. Biol. Chem. 273: 24044-24051 
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    11. Lee, S. Y., Y. Lee, and F. Wang. 1999. Chiral compounds from bacterial polyesters: Sugars to plastics to fine chemicals. Biotechnol. Bioeng. 65: 363-368 
    12. Qi, Q., A. Steinbuchel, and B. H. A. Rehm. 1998. Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinant Escherichia coli (fadR): Inhibition of fatty acid $\beta$-oxidation by acrylic acid. FEMS Microbiol. Lett. 167: 89-94 
    13. Madison, L. L. and G. W. Huisman. 1999. Metabolic engineering of poly(3-hydroxyalkanoates): From DNA to plastic. Microbiol. Mol. Biol. Rev. 63: 21-53 
    14. Lee, S. Y. and Y. Lee. 2003. Metabolic engineering of Escherichia coli for production of enantiomerically pure (R)-(-)-hydroxycarboxylic acids. Appl. Environ. Microbiol. 69: 3421-3426 
    15. Choi, M. H., H.-J. Lee, J. K. Rho, S. C. Yoon, J. D. Nam, D. B. Lim, and R. W. Lenz. 2003. Biosynthesis and local sequence specific degradation of poly(3- hydroxyvalerateco- 4-hydroxybutyrate) in Hydrogenophaga pseudoflava. Biomacromolecules 4: 38-45 
    16. Kim, D. Y., Y. B. Kim, and Y. H. Rhee. 2002. Cometabolic production of poly(3-hydroxyalkanoates) containing carboncarbon double and triple bonds by Pseudomonas oleovorans. J. Microbiol. Biotechnol. 12: 518-521 
  • 이 논문을 인용한 문헌 (1)

    1. 2007. "" Journal of microbiology and biotechnology, 17(12): 2018~2026     

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    2. 1994 "Isolation of Pseudomonas putida BM01 Accumulating High Amount of $PHA_{MCL}$" Journal of microbiology and biotechnology 4 (2): 126~133    
    3. 1994 "여러 가지 Perturbing Media에서의 알부민의 비가역적 열변성" 한국생화학회지 27 (5): 367~377    
    4. 1997 "Effect of C/N Ratio on the Production of Poly(3-hydroxyalkanoates) by the Methylotroph Paracoccus denitrificans" Journal of microbiology and biotechnology 7 (6): 391~396    
    5. 2001 "Cometabolism of $\omega$-Phenylalkanoic Acids with Butyric Acid for Efficient Production of Aromatic Polyesters in Pseudomonas putida BM01" Journal of microbiology and biotechnology 11 (3): 435~442    
    6. 2005 "In Vivo $^{13}C$-NMR Spectroscopic Study of Polyhydroxyalkanoic Acid Degradation Kinetics in Bacteria" Journal of microbiology and biotechnology 15 (6): 1330~1336    

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