본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.14 no.6, 2004년, pp.1286 - 1294   피인용횟수: 2
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Differential Gene Expression in Estradiol-3-Benzoate-Treated Liver and Chemically- Induced Hepatocellular Carcinoma

KIM , SEYL    (Department of Pathology, National Institute of Toxicology Research, Laboratory of Molecular Biology, School of Life Sciences and Biotechnology, Korea University   ); KANG, JIN-SEOK    (Department of Pathology, National Institute of Toxicology Research   ); JANG, DONG-DEUK    (Department of Pathology, National Institute of Toxicology Research   ); LEE, KOOK-KYUNG    (Department of Veterinary Medicine, Cheju National University   ); KIM, SOON-AE    (Department of Pharmacology, Eulji University School of Medicine   ); HAN, BEOM-SEOK    (Department of Pathology, National Institute of Toxicology Research   ); PARK, YOUNG-IN    (Laboratory of Molecular Biology, School of Life Sciences and Biotechnology, Korea University  );
  • 초록

    In a previous study by the current authors, hepatocellular carcinoma (HCC) was determined to be epidemiologically sex-dependent, and the incidence and multiplicity of HCC found to decrease in estradiol-3 benzoate (EB)-treated F344 rats. Therefore, to ascertain the anticancer mechanism of EB, a commercially available cDNA microarray, with a total of 14,815 cDNA rat gene clones, was used to determine the differentially expressed genes in nontreated livers, EB-treated livers, and diethynitrosolamine (DEN)-induced HCC. In the sequenced experiment, a total of 85 genes were differentially expressed at either two or more times the rate of the normal expression, where 33 genes were downregulated by EB, and 52 genes upregulated. Candidate genes were selected according to significant changes observed in the mRNA expression in the EB-treated livers compared with the nontreated livers, then these genes were filtered according to their different expression patterns in the DEN-induced tumors compared to the estrogen-treated livers. To confirm the microarray data, a real-time PCR analysis was performed for ten selected genes: the H-ras revertant protein 107 (H­rev107), insulin-like growth factor binding protein (lOFBP), parathyroid hormone receptor (PI'HR), SH3 domain binding protein (SH3BP), metallothionein, src-suppressed C-kinase substrate (SSeCK) gene, phosphodiesterase I, CD44, epithelial membrane protein 3 (EMP3), and estrogen receptor a (ERa). The SSeCK and phosphodiesterase I genes were both upregulated in the DEN-induced hepatocarcinomas, yet their possible carcinogenic functions remain unknown. Meanwhile, the other genes were downregulated, including the genes related to growth regulation (IOFBP, H-revI07, ER $\alpha$ ), adipogenesis inhibition (PTHR), and tumor suppression (metallothionein).


  • 주제어

    Hepatocellular carcinoma (HCC) .   cDNA microarray .   estradiol-3 benzoate (EB).  

  • 참고문헌 (35)

    1. Fillit, H., H. Weinreb, I. Cholst, V. Luine, B. McEwen, R. Amador, and J. Zabriskie. 1986. Observations in a preliminary open trial of estradiol therapy for senile dementia-Alzheimer’s type. Psychoneuroendocrinology 11: 337-345 
    2. La Vecchia, C., E. Negri, L. Cavalieri d’Oro, and S. Franceschi. 1998. Liver cirrhosis and the risk of primary liver cancer. Eur. J. Cancer Prev. 7: 315-320 
    3. Matsuura, B., Y. Taniguchi, and Y. Ohta. 1994. Effect of antiandrogen treatment on chemical hepatocarcinogenesis in rats. J. Hepatol. 21: 187-193 
    4. Sylvia, V. L., T. Hughes, D. D. Dean, B. D. Boyan, and Z. Schwartz. 1998. 17beta-estradiol regulation of protein kinase C activity in chondrocytes is sex-dependent and involves nongenomic mechanisms. J. Cell Physiol. 176:435-444 
    5. Xu, J. W., J. Gong, X. M. Chang, J. Y. Luo, L. Dong, Z. M. Hao, A. Jia, and G. P. Xu. 2002. Estrogen reduces CCL4-induced liver fibrosis in rats. World J. Gastroenterol. 8: 883-887 
    6. Kemp, C. J. and N. R. Drinkwater. 1989. Genetic variation in liver tumor susceptibility, plasma testosterone levels, and androgen receptor binding in six inbred strains of mice. Cancer Res. 49: 5044-5047 
    7. Dang, Z. C., R. L. van Bezooijen, M. Karperien, S. E. Papapoulos, and C. W. Lowik. 2002. Exposure of KS483 cells to estrogen enhances osteogenesis and inhibits adipogenesis. J. Bone Miner. Res. 17: 394-405 
    8. Paassilta, M., A. Karjalainen, K. Kervinen, M. J. Savolainen, J. Heikkinen, A. C. Backstrom, and Y. A. Kesaniemi. 2000. Insulin-like growth factor binding protein-1 (IGFBP-1) and IGF-I during oral and transdermal estrogen replacement therapy: Relation to lipoprotein(a) levels. Atherosclerosis 149: 157-162 
    9. Stefan, C., W. Stalmans, and M. Bollen. 1996. Threonine autophosphorylation and nucleotidylation of the hepatic membrane protein PC-1. Eur. J. Biochem. 241: 338-342 
    10. Cai, L., C. J. Wang, Z. L. Xu, D. X. Deng, S. Chakrabarti, and M. G. Cherian. 1998. Metallothionein and apoptosis in primary human hepatocellular carcinoma from northern China. Anticancer Res. 18: 4667-4672 
    11. Choi, J. Y., M. Kim, and J. H. Lee. 2002. Reevaluation of the change of leuconostoc species and Lactobacillus plantarum by PCR during kimchi fermentation. J. Microbiol. Biotechnol. 12: 166-171 
    12. Segars, J. H. and P. H. Driggers. 2002. Estrogen action and cytoplasmic signaling cascades. Part I: Membraneassociated signaling complexes. Trends Endocrinol. Metab. 13: 349-354 
    13. Shimizu, I., M. Yasuda, Y. Mizobuchi, Y. R. Ma, F. Liu, M. Shiba, T. Horie, and S. Ito. 1998. Suppressive effect of oestradiol on chemical hepatocarcinogenesis in rats. Gut 42: 112-119 
    14. Ramirez, V. D. and J. Zheng. 1996. Membrane sex-steroid receptors in the brain. Front. Neuroendocrinol. 17: 402- 439 
    15. Cicchetti, P., A. J. Ridley, Y. Zheng, R. A. Cerione, and D. Baltimore. 1995. 3BP-1, an SH3 domain binding protein, has GAP activity for Rac and inhibits growth factor-induced membrane ruffling in fibroblasts. EMBO J. 14: 3127-3135 
    16. Deng, D. X., S. Chakrabarti, M. P. Waalkes, and M. G. Cherian. 1998. Metallothionein and apoptosis in primary human hepatocellular carcinoma and metastatic carcinoma. Histopathology 32: 348-355 
    17. Lin, X., P. Nelson, and I. H. Gelman. 2000. SSeCKS, a major protein kinase C substrate with tumor suppressor activity, regulates G13S progression by controlling the expression and cellular compartmentalization of cyclin D. Mol. Cell. Biology 20: 7259-7272 
    18. Sawaki, M., K. Enomoto, H. Takahashi, Y. Nakajima, and M. Mori. 1990. Phenotype of preneoplastic and neoplastic liver lesions during spontaneous liver carcinogenesis of LEC rats. Carcinogenesis 11: 1857-1861 
    19. Weber, G. F., R. T. Bronson, J. Ilagan, H. Cantor, R. Schmits, and T. W. Mak. 2002. Absence of the CD44 gene prevents sarcoma metastasis. Cancer Res. 62: 2281-2286 
    20. Jeoung, D., J. H. Kim, Y. H. Lee, M. Baek, S. Lee, N. I. Baek, and H. Y. Kim. 2002. cDNA microarray analysis of transcriptional response to hyperin in human gastric cancer cells. J. Microbiol. Biotechnol. 12: 664-668 
    21. Colditz, G. A., K. M. Egan, and M. J. Stampfer. 1993. Hormone replacement therapy and risk of breast cancer: Results from epidemiologic studies. Am. J. Obstet. Gynecol. 168: 1473-1480 
    22. Even, C., G. Launoy, T. Collet, O. Duval, M. A. Piquet, A. Rougereau, J. C. Verwaerde, and T. Dao. 1997. Epidemiology of hepatocellular carcinoma in the department of Calvados. Gastroenterol. Clin. Biol. 21: 450-458 
    23. Horwitz, K. B., Y. Koseki, and W. L. McGuire. 1978. Estrogen control of progesterone receptor in human breast cancer: Role of estradiol and antiestrogen. Endocrinology 103: 1742-1751 
    24. Watson, P. H., L. J. Fraher, G. N. Hendy, U. I. Chung, M. Kisiel, B. V. Natale, and A. B. Hodsman. 2000. Nuclear localization of the type 1 PTH/PTHrP receptor in rat tissues. J. Bone Miner. Res. 15: 1033-1044 
    25. Pizzuti, A., L. Frittitta, A. Argiolas, R. Baratta, I. D. Goldfine, M. Bozzali, T. Ercolino, G. Scarlato, L. Iacoviello, R. Vigneri, V. Tassi, and V. Trischitta. 1999. A polymorphism (K121Q) of the human glycoprotein PC-1 gene coding region is strongly associated with insulin resistance. Diabetes 48: 1881-1884 
    26. Yu, M. C., J. M. Yuan, S. Govindarajan, and R. Ross. 2000. Epidemiology of hepatocellular carcinoma. Can. J. Gastroenterol. 14: 703-709 
    27. Roder, K., K. H. Kim, and H. S. Sul. 2002. Induction of murine H-rev107 gene expression by growth arrest and histone acetylation: Involvement of an Sp1/Sp3-binding GCboxq. Biochem. Biophys. Res. Commun. 294: 63-70 
    28. Erdstein, J., S. Wisebord, S. Y. Mishkin, and S. Mishkin. 1989. The effect of several sex steroid hormones on the growth rate of three Morris hepatoma tumor lines. Hepatology 9: 621-624 
    29. Nelson, P. and I. H. Gelman. 1997. Cell-cycle regulated expression and serine phosphorylation of the myristylated protein kinase C substrate, SSeCKS: Correlation with cell confluency, G0 phase and serum response. Mol. Cell. Biochem. 175: 233-241 
    30. Yue, S. Q., Y. L. Yang, K. F. Dou, and K. Z. Li. 2003. Expression of PCNA and CD44 mRNA in colorectal cancer with venous invasion and its relationship to liver metastasis. World J. Gastroenterol. 9: 2863-2865 
    31. Henderson, B. E., R. K. Ross, A. Paganini-Hill, and T. M. Mack. 1986. Estrogen use and cardiovascular disease. Am. J. Obstet. Gynecol. 154: 1181-1186 
    32. Hoe, H. S., I. G. Jo, H. J. Shin, H. J. Jeon, H. K. Kim, J. S. Lee, Y. S. Kim, D. S. Lee, and S. T. Kwon. 2003. Cloning and expression of the gene for inorganic pyrophosphatase of Thermus caldophilus GK24 and properties of the enzyme. J. Microbiol. Biotechnol. 13: 139-145 
    33. Mishkin, S. Y., E. Farber, R. K. Ho, S. Mulay, and S. Mishkin. 1983. Evidence for the hormone dependency of hepatic hyperplastic nodules: Inhibition of malignant transformation after exogenous 17 beta-estradiol and tamoxifen. Hepatology 3: 308-316 
    34. Turner, R. T., B. L. Riggs, and T. C. Spelsberg. 1994. Skeletal effects of estrogen. Endocr. Rev. 15: 275-300 
    35. Mackay, A., C. Jones, T. Dexter, R. L. Silva, K. Bulmer, A. Jones, P. Simpson, R. A. Harris, P. S. Jat, A. M. Neville, L. F. Reis, S. R. Lakhani, and M. J. O’Hare. 2003. cDNA microarray analysis of genes associated with ERBB2 (HER2/neu) overexpression in human mammary luminal epithelial cells. Oncogene 22: 2680-2688 
  • 이 논문을 인용한 문헌 (2)

    1. 2006. "" Journal of microbiology and biotechnology, 16(12): 1841~1848     
    2. 2006. "" Journal of microbiology and biotechnology, 16(8): 1292~1300     

 저자의 다른 논문

  • PARK, YOUNG-IN (32)

    1. 1991 "E.coli K-12 균주로부터 글루타치온 합성 유전자의 클로닝" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 19 (6): 575~582    
    2. 1992 "Bacillus stearothermophilus로 부터 Endo-xylanase 유전자의 클로닝 및 Escherichia coli에서의 발현" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 20 (3): 271~279    
    3. 1992 "Bacillus stearothermophilus $\beta$-D-Xylosidase 유전자의 크로닝 및 Escherichia coli에서의 발현" 산업미생물학회지 = Korean journal of applied microbiology and biotechnology 20 (2): 136~142    
    4. 1995 "In vivo Angiogenic Activity of Dichloromethane Extracts of Aloe vera Gel" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 18 (5): 332~335    
    5. 1997 "수종 생약의 티로시나제 억제효과" 약학회지 = Yakhak hoeji 41 (4): 518~523    
    6. 1998 "The Inhibitory Mechanism of Aloe Glycoprotein (NY945) on the Mediator Release in the Guinea Pig Lung Mast Cell Activated with Antigen-Antibody Complexes" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2 (1): 119~131    
    7. 1998 "The Glycopeptide, a Promoter of Thymidine Uptake, from Aloe Vera" Natural product sciences 4 (2): 62~67    
    8. 1998 "In vitro Angiogenic Activity of Aloe vera Gel on Calf Pulmonary Artery Endothelial (CPAE) Cells" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 21 (3): 260~265    
    9. 1999 "Aloesin and Arbutin Inhibit Typrosinase Activity in a Synergistic Manner via a Different Action Mechanism" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 22 (3): 232~236    
    10. 1999 "Transgenic Tobacco Plants Expressing the Bacterial Levansucrase Gene Show Enhanced Tolerance to Osmotic Stress" Journal of microbiology and biotechnology 9 (2): 213~218    

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기