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Journal of microbiology and biotechnology v.14 no.6, 2004년, pp.1295 - 1302   피인용횟수: 6
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Cloning of a Bacillus subtilis WL-7 Mannanase Gene and Characterization of the Gene Product

KWEUN , MIN-A    (School of Food Science and Biotechnology, Woosong University   ); LEE, MI-SUNG    (School of Food Science and Biotechnology, Woosong University   ); CHOI, JOON-HO    (R&D Center, KoBioTech Co.   ); CHO, KI-HAENG    (R&D Center, CTCBIO Inc.   ); YOON, KI-HONG    (School of Food Science and Biotechnology, Woosong University, Bioresouce and Application Research Center, Woosong University  );
  • 초록

    A gene encoding the mannanase of Bacillus subtilis WL-7, which had been isolated from Korean soybean paste, was cloned into Escherichia coli, and the gene product was purified from the culture filtrate of the recombinant E. coli. This mannanase gene, designated manA, consisted of 1,086 nucleotides, encoding a polypeptide of 362 amino acid residues. The deduced amino acid sequence was highly homologous to those of mannanases belonging to the glycosyl hydrolase family 26. The molecular mass of the purified mannanase was 38 kDa as estimated by SDS-PAGE. The enzyme had a pH optimum at 6.0 and a temperature optimum at $55^{\circ}C$ . The enzyme was active on locust bean gum, konjak, guar gum, and lichenan, while it did not exhibit activity towards yeast mannan, laminarin, carboxymethylcellulose, $\beta$ ­glucan, xylan, and para-nitrophenyl- $\beta$ -mannopyranoside.


  • 주제어

    Bacillus subtilis WL-7 .   mannanase .   gene .   purification .   recombinant Escherichia coli.  

  • 참고문헌 (46)

    1. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248- 254 
    2. Cann, I. K., S. Kocherginskaya, M. R. King, B. A. White, and R. I. Mackie. 1999. Molecular cloning, sequencing, and expression of a novel multidomain mannanase gene from Thermoanaerobacterium polysaccharolyticum. J. Bacteriol. 181: 1643-1651 
    3. Christgau, S., S. Kauppinen, J. Vind, L.-V. Koffod, and H. Dalboge. 1994. Expression cloning, purification and characterization of a $\beta$-1,4-mannanase from Aspergillus aculeatus. Biochem. Mol. Biol. Int. 33: 917-925 
    4. Kataoka, N. and Y. Tokiwa. 1998. Isolation and characterization of an active mannanase-producing anaerobic bacterium, Clostridium tertium KT-5A, from lotus soil. J. Appl. Microbiol. 84: 357-367 
    5. Khanongnuch, C., T. Ooi, and S. Kinoshita. 1999. Cloning and nucleotide sequence of $\beta$-mannanse and cellulase genes from Bacillus sp. 5H. Wor. J. Microbiol. Biotechnol. 15: 249-258 
    6. Kim, K. C., S.-S. Yoo, Y.-A. Oh, and S.-J. Kim. 2003. Isolation and characteristics of Trichoderma harzianum FJ1 producing cellulases and xylanase. J. Microbiol. Biotechnol. 13: 1-8 
    7. Kim, M. D., K.-N. Kim, and Y. J. Choi. 2003. Enhanced expression of $\beta$-xylosidase of Bacillus stearothermophilus No. 236 by change of translational initiation codon in Escherichia coli and Bacillus subtilis. J. Microbiol. Biotechnol. 13: 584-590 
    8. Kurokawa, J., E. Hemjinda, T. Arai, S. Karita, T. Kimura, K. Sakka, and K. Ohmiya. 2001. Sequence of the Clostridium thermocellum mannanase gene man26B and characterization of the translated product. Biosci. Biotechnol. Biochem. 65: 548-554 
    9. Mendoza, N. S., M. Arai, K. Sugimoto, M. Ueda, T. Kawaguchi, and L. M. Joson. 1995. Cloning and sequencing of $\beta$-mannanase gene from Bacillus subtilis NM-39. Biochim. Biophys. Acta 1243: 552-554. 
    10. Parker, K. N., S. R. Chhabra, D. Lam, W. Callen, G. D. Duffaud, M. A. Snead, J. M. Short, E. J. Mathur, and R. M. Kelly. 2001. Galactomannanases Man2 and Man5 from Thermotoga species: growth physiology on galactomannans, gene sequence analysis, and biochemical properties of recombinant enzymes. Biotechnol. Bioeng. 75: 322-333 
    11. Puls, J. and J. Schuseil. 1996. Chemistry of hemicelluloses: Relationship between hemicellulose structure and enzymes required for hydrolysis, pp. 1-28. In M. P. Coughlan and G. P. Hazlewood (eds.). Hemicelluloses and Hemicellulases. Portland Press, London, England 
    12. Sunna, A., M. D. Gibbs, C. W. J. Chin, P. J. Nelson, and P. L. Bergquist. 1999. A gene encoding a novel multidomain $\beta$-1,4-mannanase from Caldibacillus cellulovorans and action of the recombinant enzyme on kraft pulp. Appl. Environ. Microbiol. 66: 664-670 
    13. Tamaru, Y. and R. H. Doi. 2000. The engL gene cluster of Clostridium cellulovorans contains a gene for cellulosomal ManA. J. Bacteriol. 182: 244-247 
    14. Valentine, P. J. and A. A. Salyers. 1992. Analysis of proteins associated with growth of Bacteroides ovatus on the branched galactomannan guar gum. Appl. Environ. Microbiol. 58: 1534-1540 
    15. Johnvesly, B., S. Virupakshi, G. N. Patil, Ramalingam, and G. R. Naik. 2002. Cellulase-free thermostable alkaline xylanase from thermophilic and alkalophilic Bacillus sp. JB-99. J. Microbiol. Biotechnol. 12: 153-156 
    16. Mendoza, N. S., M. Arai, T. Kawaguchi, T. Yoshida, and L. M. Joson. 1994. Purification and properties of mannanase from Bacillus subtilis. Wor. J. Microbiol. Biotechnol. 10: 551-556 
    17. Sharp, P. M., D. G. Higgins, D. C. Shields, and K. M. Devine. 1990. Protein-coding genes: DNA sequence database and codon usage, pp. 557-569. In C. R. Harwood and S. M. Cutting (eds.). Molecular Biological Methods for Bacillus. John Wiley & Sons, Chichester, England 
    18. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. Basic local alignment search tool. J. Mol. Biol. 215: 403-410 
    19. Grosjean, H. and W. Fiers. 1982. Preferential codon usage in genes: The optimal codon-anticodon interaction energyand the selective codon usage in efficiently expressed genes. Gene 18: 199-209 
    20. Hogg, D., G. Pell, P. Dupree, F. Goubet, S. M. Martin-Orue, S. Armand, and H. J. Gilbert. 2003. The modular architecture of Cellvibrio japonicus mannanases in glycoside hydrolase families 5 and 26 points to differences in their role in mannan degradation. Biochem. J. 371: 1027-1043 
    21. Kweun, M. A., H. S. Kim, M.-S. Lee, J. H. Choi, and K.-H. Yoon. 2003. Mannanase production by a soybean isolate, Bacillus subtilis WL-7. Kor. J. Microbiol. Biotechnol. 31: 277-283 
    22. Stoll, D., H. Stalbrand, and R. A. Warren. 1999. Mannandegrading enzymes from Cellulomonas fimi. Appl. Environ. Microbiol. 65: 2598-2605 
    23. Arcand, N., D. Kluepfel, F. W. Paradis, R. Morosoli, and F. Shareck. 1993. $\beta$-Mannanase of Streptomyces lividans 66: Cloning and DNA sequence of the manA gene and characterization of the enzyme. Biochem. J. 290: 857-863 
    24. Kansoh, A. L. and Z. A. Nagieb. 2004. Xylanase and mannanase enzymes from Streptomyces galbusNR and their use in biobleaching of softwood kraft pulp. Antonie van Leeuwenhoek 85: 103-114 
    25. Politz, O., M. Krah, K. K. Thomsen, and R. Borriss. 2000. A highly thermostable endo-(1,4)-$\beta$-mannanase from the marine bacterium Rhodothermus marinus. Appl. Microbiol. Biotechnol. 53: 715-721 
    26. Tamaru, Y., T. Araki, H. Amagoi, H. Mori, and T. Morishita. 1995. Purification and characterization of an extracellular $\beta$-1,4-mannanase from a marine bacterium, Vibrio sp. strain MA-138. Appl. Environ. Microbiol. 61: 4454-4458 
    27. Duffaud G. D., C. M. McCutchen, P. Leduc, K. N. Parker, and R. M. Kelly. 1997. Purification and characterization of extremely thermostable $\beta$-mannanase, $\beta$-mannosidase, and $\alpha$-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068. Appl. Environ. Microbiol. 63: 169-177. 
    28. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning. A Laboratory Mannual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A 
    29. Araujo, J. and O. P. Ward. 1990. Production, purification and properties of the mannanase components of Bacillus pumilus. Appl. Environ. Microbiol. 56: 1954-1956 
    30. Talbot, G. and J. Sygusch. 1990. Purification and characterization of thermostable $\beta$-mannanase and $\alpha$-galactosidase from Bacillus stearothermophilus. Appl. Environ. Microbiol. 56: 3505-3510 
    31. Fanutti, C., T. Ponyi, G. W. Black, G. P. Hazlewood, and H. J. Gilbert. 1995. The conserved noncatalytic 40-residue sequence in cellulases and hemicellulases from anaerobic fungi functions as a protein docking domain. J. Biol. Chem. 270: 29314-29322 
    32. Oda, Y., T. Komaki, and K. Tonomura. 1993. Purification and properties of extracellular $\beta$-mannanases produced by Enterococcus casseliflavus FL2121 isolated from decayed konjac. J. Ferment. Bioeng. 76: 14-18 
    33. Sadaie, Y., K. Yata, M. Fujita, H. Sagai, M. Itaya, Y. Kosahara, and N. Ogasawara. 1997. Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome. Microbiology 143: 1861-1866 
    34. Gibbs, M. D., A. U. Elinder, R. A. Reeves, and P. L. Bergquist. 1996. Sequencing, cloning and expression of a$\beta$-1,4- mannanase gene, manA, from the extremely thermophilic anaerobic bacterium, Caldicellulosiruptor Rt8B.4. FEMS Microbiol. Lett. 141: 37-43 
    35. Montiel, M. D., M. Hernandez, J. Rodriguez, and M. E. Arias. 2002. Evaluation of an endo-$\beta$-mannanase produced by Streptomyces ipomoea CECT 3341 for the biobleaching of pine kraft pulps. Appl. Microbiol. Biotechnol. 58: 67-72 
    36. Kim, S. W., D. A. Knabe, K. J. Hong, and R. A. Easter. 2003. Use of carbohydrases in corn-soybean meal-based nursery diets. J. Anim. Sci. 81: 2496-2504 
    37. Miller, M. L., R. Blum, W. E. Glennon, and A. L. Burton. 1960. Measurement of carboxymethylcellulase activity. Anal. Biochem. 2: 127-132 
    38. Rodriquez, R. L. and R. C. Tait. 1983. Recombinant DNA Techniques - An Introduction, pp. 45-46. Addison-Wesley Pub. Co., Reading, MA, U.S.A 
    39. Akino, T., C. Kato, and K. Horikoshi. 1989. The cloned $\beta$-mannanase gene from alkalophilic Bacillus sp. AM-001 produced two$\beta$-mannanases in Escherichia coli. Arch. Microbiol. 152: 10-15 
    40. Hagglund, P., T. Eriksson, A. Collen, W. Nerinckx, M. Claeyssens, and H. Stalbrand. 2003. A cellulose-binding module of the Trichoderma reesei $\beta$-mannanase Man5A increases the mannan-hydrolysis of complex substrates. J. Biotechnol. 101: 37-48 
    41. Ademark, P., A. Varga, J. Medve, V. Harjunpaa, T. Drakenberg, F. Tjerneld, and H. Stalbrand. 1998. Softwood hemicellulose-degrading enzymes from Aspergillus niger: Purification and properties of a $\beta$-mannanase. J. Biotechnol. 63: 199-210. 
    42. Gibbs, M. D., R. A. Reeves, A. Sunna, and P. L. Bergquist. 1999. Sequencing and expression of a $\beta$-mannanase gene from the extreme thermophile Dictyoglomus thermophilum Rt46B.1, and characteristics of the recombinant enzyme. Curr. Microbiol. 39: 351-357 
    43. Stoll, D., A. Boraston, H. Stalbrand, B. W. McLean, D. G. Kilburn, and R. A. J. Warren. 2000. Mannanase Man26A from Cellulomonas fimi has a mannan-binding module. FEMS Microbiol. Lett. 183: 265-269 
    44. Yamaura, L., T. Matsumoto, M. Funatsu, and Y. Funatsu. 1990. Purification and some properties of endo-1,4-$\beta$-Dmannanase from Pseudomonas sp. PT-5. Agric. Biol. Chem. 54: 2425-2427 
    45. Kim, Y.-H., M.-N. Gwon, S.-Y. Yang, T.-K. Park, C.-G. Kim, C.-W. Kim, and M.-D. Song. 2002. Isolation of phytaseproducing Pseudomonas sp. and optimization of its phytase production. J. Microbiol. Biotechnol. 12: 279-285 
    46. Morris, D. D., R. A. Reeves, M. D. Gibbs, D. J. Saul, and P. L. Bergquist. 1995. Correction of the $\beta$-mannanase domain of the celC pseudogene from Caldocellulosiruptor saccharolyticus and activity of the gene product on kraft pulp. Appl. Environ. Microbiol. 61: 2262-2269 
  • 이 논문을 인용한 문헌 (6)

    1. 2007. "" Journal of microbiology and biotechnology, 17(10): 1688~1694     
    2. Yoon, Ki-Hong 2010. "Cloning and Characterization of Mannanase Gene from Bacillus subtilis WL-8" Korean journal of microbiology = 미생물학회지, 46(2): 207~212     
    3. Yoon, Ki-Hong 2010. "Characterization of the Bacillus licheniformis WL-12 Mannanase from a Recombinant Escherichia coli" Journal of applied biological chemistry, 53(2): 71~76     
    4. Jeon, Ho Jin ; Yoon, Ki-Hong 2014. "Comparison of Acidic pH and Temperature Stabilities between Two Bacillus Mannanases Produced from Recombinant Escherichia coli" Korean journal of microbiology = 미생물학회지, 50(4): 327~333     
    5. Yoon, Ki-Hong 2016. "Molecular cloning and characterization of β-mannanase B from Cellulosimicrobium sp. YB-43" Korean journal of microbiology = 미생물학회지, 52(3): 336~343     
    6. Yoon, Ki-Hong 2016. "Isolation of Mannanase-producing Bacteria, Bacillus subtilis WL-6 and WL-11, and Cloning and Characterization of Mannanase" 생명과학회지 = Journal of life science, 26(10): 1113~1120     

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