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Journal of microbiology and biotechnology v.14 no.6, 2004년, pp.1303 - 1309   피인용횟수: 3
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Development of Antimicrobial Edible Film from Defatted Soybean Meal Fermented by Bacillus subtilis

KIM , HYUNG-WOOK    (Department of Applied Biology and Chemistry, Konkuk University   ); KIM, KYUNG-MI    (Department of Applied Biology and Chemistry, Konkuk University   ); KO, EUN-JUNG    (Department of Applied Biology and Chemistry, Konkuk University   ); LEE, SI-KYUNG    (Department of Applied Biology and Chemistry, Konkuk University   ); HA, SANG-DO    (Department of Food Science and Technology, Chung-Ang University   ); SONG, KYUNG-BIN    (Department of Food Science and Technology, Chungnam National University   ); PARK, SANG-KYU    (Material Science and Engineering, Kwangju Institute of Science and Technology   ); KWON, KI-SUNG    (Korea Food and Drug Administration   ); BAE, DONG-HO    (Department of Applied Biology and Chemistry, Konkuk University  );
  • 초록

    In order to extend shelf-life of the packaged or coated foods, an antibacterial edible film was developed. Antimicrobial activities of 9 bacteriocin-like substance (BLS)­producing strains were evaluated after growing them on defatted soybean meal medium (DSMM). Bacillus subtilis was selected among those, because it showed the biggest inhibition zone against 6 problem bacteria in food. The antimicrobial edible film, containing $0.32\%$ of BLS, was produced from the fermented soybean meal with B. subtilis at the optimum condition of pH 7.0-7.5 and $33^{\circ}C$ for 33 h. The antimicrobial activity of the film was over $50\%$ of the maximum activity after film production with heat treatment at $90^{\circ}C$ and pH adjustment to 9. When the soy protein film with BLS was applied on the agar media containing E. coli, the growth inhibition was much higher than the ordinary soy protein film. These results indicate that the soy protein film with BLS from B. subtilis can be used as a new packaging material to extend the shelf-life of foods.


  • 주제어

    Bacteriocin-like substance (BLS) .   functional film .   antimicrobial activity .   B.   subtilis .   soybean meal.  

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