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Journal of microbiology and biotechnology v.14 no.6, 2004년, pp.1310 - 1317   피인용횟수: 2
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Process Strategies to Enhance the Production of 5-Aminolevulinic Acid with Recombinant E. coli

LEE , DAE-HEE    (Department of Food and Biotechnology, School of Life Sciences and Biotechnology, Korea University   ); JUN, WOO-JIN    (Department of Food and Nutrition, Chonnam National University   ); YOON, JEONG-WEON    (Department of Bioscience and Biotechnology, Suwon University   ); CHO, HONG-YON    (Department of Food and Biotechnology, School of Life Sciences and Biotechnology, Korea University   ); HONG, BUM-SHIK    (Department of Food and Biotechnology, School of Life Sciences and Biotechnology, Korea University  );
  • 초록

    The extracellular production of 5-aminolevulinic acid (ALA) by recombinant E. coli BL21 harboring a fusion gene hemA was investigated in a fermenter. For this purpose, the effects of various physiological factors, such as isopropylthio­ $\beta$ -D-galactopyranoside (IPTG) concentrations and the time of induction, on enzyme activity were studied. Optimum concentrations of glycine and succinic acid were found to be 30 mM and 90 mM, respectively. When the cells were permitted to grow for 2 h prior to the addition of 0.1 mM IPTG, the activity of ALA synthase was higher than when IPTG was initially added. A 36-fold increase in the activity was observed with only 0.1 mM IPTG added. The pH of the medium also influenced the ALA synthase activity with the maximal activity occurring at pH 6.5. In recombinant E. coli extracts, the repeated addition of glycine and D-glucose increased the production of ALA and the inhibited intracellular ALA dehydratase activity, with up to 32 mM ALA being produced in the cultivation.

  • 주제어

    5-Aminolevulinic acid .   aminolevulinic acid synthase .   aminolevulinic acid dehydratase .   levulinic acid .   D-glucose.  

  • 참고문헌 (41)

    1. Baca-DeLancey, R. R., M. M. T. South, X. Doing, and P. N. Rather. 1999. Escherichia coli genes regulated by cell-to-cell signaling. Proc. Natl. Acad. Sci. USA 96: 4610-4614 
    2. Gong, J., G. A. Hunters, and G. C. Ferreira. 1998. Aspartate 279 in aminolevulinic acid synthase affects enzyme catalysis through enhancing the function of the pyridoxal 5-phosphate cofactor. Biochemistry 37: 3509-3517 
    3. Hotta, Y., T. Tanaka, H. Takaoka, Y. Takeuchi, and M. Konnai. 1997. New physiological effects of 5-aminolevulinic acid in plants: The increase of photosynthesis, chlorophyll content, and plant growth. Biosci. Biotechnol. Biochem. 61: 2025-2028 
    4. Ishino, A., A. Magara, M. Tajima, Y. Tuji, K. Takahashi, T. Tanaka, and Y. Hotta. 1999. External medicine for head hair. Jpn Kokai Tokkyo Koho: Toku Kai Hei 11-116446 
    5. Laemmli, U. K. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227: 680-685 
    6. Lee, D. H., W. J. Jun, K. M. Kim, D. H. Shin, H. Y. Cho, and B. S. Hong. 2003. Inhibition of 5-aminolevulinic acid dehydratase in recombinant Escherichia coli using Dglucose. Enzym. Microb. Tech. 32: 27-34 
    7. Matsumoto, H., Y. Tanida, and K. Ishizuka. 1994. Pesticide Biochem. 48: 214-219 
    8. Mauzerall, S. and S. Granick, S. 1956. The occurrence and determination of $\delta$-aminolevulinic acid and porphobilinogen in urine. J. Biol. Chem. 219: 435-442 
    9. Neidle, E. L. and S. Kaplan. 1993. Expression of the Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthase isozymes. J. Bacteriol. 175: 2292-2303 
    10. Peng, Q., H. F. Berg, J. Moan, M. Kongshaug, and J. M. Neslang. 1997. 5-Aminolevulinic acid-based photodynamic therapy: Principle and experimental research. Photochem. Photobiol. 65: 235-251 
    11. Rebeiz, C. A., A. Montazer-Zouhoor, J. M. Mayasich, B. C. Tripathy, S. M. Wu, and C. C. Rebeiz. 1988. Photodynamic herbicides. Recent developments and molecular basis of selectivity. Crit. Rev. Plant Sci. 6: 385-436 
    12. Lascelles, J. 1978. Regulation of pyrrole synthesis, pp. 795- 808. In R. K. Clayton and W. R. Sistrom (eds.), The Photosynthetic Bacteria. Plenum Press, New York. U.S.A 
    13. Lowry, O. H., N. J. Roenbrough, A. L. Farr, and R. J. Randal. 1951. Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265-275 
    14. McClung, C. R., J. E. Somerville, M. L. Guerinot, and B. K. Chelm. 1987. Structure of the Bradyrhizobium japonicum gene hemA encoding 5-aminolevulinic acid synthase. Gene 54: 133-139 
    15. Vladimir, Y. B., A. L. Demain, and N. I. Zaitseva. 1997. The crucial contribution of starved resting cells to the elucidation of the pathway of vitamin B12 biosynthesis. Crit. Rev. Biotechnol. 17: 21-37 
    16. Burnham, B. F. 1970.TEX>$\delta$-Aminolevulinic acid synthase (Rhodopseudomonas sphaeroides). Methods Enzymol. 17A: 195-200 
    17. Cho, Y. S., L. D. Park, Y. W. Kim, H. Hwangbo, W. J. Jung, J. S. Shu, B. S. Koo, H. B. Krishna, and K. Y. Kim. 2003. PQQ-Dependent organic acid production and effect on common bean growth by Rhizobium tropici CIAT 899. J. Microbiol. Biotechnol. 13(6): 955-959 
    18. Mariet J van der Werf and J. Gregory Zeikus. 1996. 5- Aminolevulinate production by Escherichia coli containing the Rhodobacter sphaeroides hemA gene. Appl. Environ. Microbiol. 62: 3560-3566 
    19. Rebeiz, C. A., J. A. Juvik, and C. C. Rebeiz. 1988. Porphyric insecticides. 1. Concept and phenomenology. Pestic. Biochem. Physiol. 30: 11-27 
    20. Hunters, G. and G. C. Ferreira. 1999. Pre-steady-state reaction of 5-aminolevulinic acid synthase. J. Biol. Chem. 274: 12222-12228 
    21. Mitchell, L. W. and E. K. Jaffe. 1993. Porphobilinogen synthase from Escherichia coli is a Zn(II) metalloenzyme stimulated by Mg(II). Arch. Biochem. Biophys. 300: 169- 177 
    22. Nandi, D. L. and D. Shemin. 1968. Delta-aminolevulinic acid dehydratase of Rhodopseudomonas spheroides. 3. Mechanism of porphobilinogen synthesis. J. Biol. Chem. 243: 1236-1242 
    23. Glick, B. R. 1995. Metabolic load and heterologous gene expression. Biotechnol. Adv. 13: 247-261 
    24. Levy, J. G. 1995. Photodynamic therapy. Trends Biotechnol. 13: 14-18 
    25. Malik, Z., J. Hanania, and Y. Nitzan. 1990. New trends in photobiology. Bactericidal effects of photoactivated porphyrinsan alternative approach to antimicrobial drugs. J. Photochem. Photobiol. B. Biol. 5: 281-293 
    26. Sasaki, K., T. Tanaka, N. Nishio, and S. Nagai. 1993. Effect of culture pH on the extracellular production of $\delta$-aminolevulinic acid by Rhodobacter sphaeroides from volatile fatty acids. Biotech. Lett. 15: 859-864 
    27. Jaffe, E. K. and S. Rajagopalan. 1990. Nuclear magnetic resonance studies of 5-aminolevulinate demonstrate multiple forms in aqueous solution. Bioorg. Chem. 18: 381-394 
    28. Oh, K. S., D. K. Na, M. H. Kweon, and H. J. Sung. 2003. Expression and purification of delta sleep-inducing peptide in Escherichia coli. J. Microbiol. Biotechnol. 13(4): 620- 623 
    29. Kriegmair, M., R. Baumgartner, R. Kneuchei, H. Stepp, F. Hofstadter, and A. Pofstertter. 1996. Detection of early bladder cancer by 5-aminolevulinic acid induced porphyrin fluorescence. J. Urology 165: 105-110 
    30. Beale, S. I. 1978. $\delta$-Aminolevulinic acid in plants: Its biosynthesis, regulation, and role in plastid development. Ann. Rev. Physiol. 29: 95-120 
    31. Jordan, P. M. 1991. Biosynthesis of Tetrapyrroles. New Comprehensive Biochemistry. Elsevier, Amsterdam. 19: 1-24 
    32. Donovan, R. S., C. W. Robinson, and B. R. Glick. 2000. Optimizing the expression of a monoclonal antibody fragment under the transcriptional control of the Escherichia coli lac promoter. Can. J. Microbiol. 46: 532-541 
    33. Klotsky, R. A. and I. Schwartz. 1987. Measurement of cat expression from growth-rate-regulated promoters employing $\beta$-lactamase activity as an indicator of plasmid copy number. Gene 55: 141-146 
    34. Butler, A. R. and S. George. 1992. The nonenzymatic cyclic dimerisation of 5-aminolevulinic acid. Tetrahedron 48: 7879-7886 
    35. Takeya, H., T. Tanaka, T. Hotta, and K. Sasaki. 1997. Production methods and applications of 5-aminolevulinic acid. Porphyrins 6: 127-135 
    36. Kosinski, M. J., U. Rinas, and J. E. Bailey. 1992. Isopropylb- d-thiogalactopyranoside influences the metabolism of Escherichia coli. Appl. Microbiol. Biotechnol. 36: 782- 783 
    37. Sasikala, C., C. V. Ramana, and R. P. Raghuveer. 1994. 5- Aminolevulinic acid: A potential herbicide/insecticide from microorganisms. Biotechnol. Prog. 10: 451-459 
    38. Nandi, D. L. and D. Shemin. 1997. Quaternary structure of 5-aminolevulinic acid synthase from Rhodopseudomonas sphaeroides. J. Biol. Chem. 252: 2278-2280 
    39. Sasaki, K., T. Tananka, Y. Nishizawa, and M. Hayashi. 1991. Enhanced production of 5-aminolevulinic acid by repeated addition of levulinic acid and supplement of precursors in photoheterotrophic culture of Rhodobacter sphaeroides. J. Ferment. Bioeng. 71: 403-406 
    40. Watanabe, K., T. Tanaka, Y. Hotta, H. Kuramochi, and Y. Takeuchi. 2000. Improving salt tolerance of cotton seedlings with 5-aminolevulinic acid. Plant Growth Regul. 32: 97- 101 
    41. Choi, C., B. S. Hong, H. Y. Sung, H. S. Lee, and J. H. Kim. 1999. Optimization of extracellular 5-aminolevulinic acid production from Escherichia coli transformed with ALA synthase gene of Bradyrhizobium japonicum. Biotech. Lett. 21: 551-554 
  • 이 논문을 인용한 문헌 (2)

    1. 2006. "" Journal of microbiology and biotechnology, 16(9): 1448~1452     
    2. Yang, Dong-Soo ; Park, Moon-Won ; Lim, Soo-Jin ; Kim, Min-Jeong ; Shin, Yu-Ri ; Park, Chan-Soo ; Hyun, Young ; Kang, Dae-Kyung 2009. "Optimizing the Production of 5-Aminolevulinic Acid by Recombinant Escherichia coli Containing the Rhodobacter capsulatus hemA Gene" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 37(2): 153~159     

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