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Journal of microbiology and biotechnology v.14 no.6, 2004년, pp.1318 - 1323   피인용횟수: 15
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Characterization of Antihypertensive Angiotensin I-Converting Enzyme Inhibitor from Saccharomyces cerevisiae

KIM, JAE-HO    (Bae Sang Myun Brewery Co., Ltd.   ); LEE, DAE-HYOUNG    (Department of Genetic Engineering and Bio-Medical Resource Research Center, Paichai University   ); JEONG, SEOUNG-CHAN    (Department of Genetic Engineering and Bio-Medical Resource Research Center, Paichai University   ); CHUNG, KUN-SUB    (Department of Biological Resources and Technology, Yonsei University   ); LEE, JONG-SOO    (Department of Genetic Engineering and Bio-Medical Resource Research Center, Paichai University  );
  • 초록

    This study describes the purification and characterization of a novel antihypertensive angiotensin 1­converting enzyme (ACE) inhibitory peptide from Saccharomyces cerevisiae. Maximal production of the ACE inhibitor from Saccharomyces cerevisiae was obtained from 24 h of cultivation at $30^{\circ}C$ and its ACE inhibitory activity was increased by about 1.5 times after treatment of the cell-free extract with pepsin. After the purification of ACE inhibitory peptides with ultrafiltration, Sephadex G-25 column chromatography, and reverse-phase HPLC, an active fraction with an $IC_{50}$ of 0.07 mg and $3.5\%$ yield was obtained. The purified peptide was a novel decapeptide, showing very low similarity to other ACE inhibitory peptide sequences, and its amino acid sequence was Tyr-Asp-Gly-Gly-Val-Phe-Arg-Val-Tyr-Thr. The purified inhibitor competitively inhibited ACE and also showed a clear antihypertensive effect in spontaneously hypertensive rats (SHR) at a dosage of 1 mg/kg body weight.


  • 주제어

    Antihypertension .   Saccharomyces cerevisiae .   angiotensin I-converting enzyme inhibitor.  

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  • 이 논문을 인용한 문헌 (15)

    1. 2007. "" Journal of microbiology and biotechnology, 17(3): 530~533     
    2. Song, Jung-Hwa ; Jang, Jeong-Hoon ; Na, Kwang-Chul ; Kim, Ha-Kun ; Lee, Jong-Soo 2010. "Screening of Yeast for Brewing of Korean Traditional Pear Yakju and Optimal Fermentation Condition" 한국균학회지 = The Korean journal of mycology, 38(2): 184~188     
    3. Jang, Jeong-Hoon ; Kim, Jae-Ho ; Kim, Na-Mi ; Kim, Ha-Kun ; Lee, Jong-Soo 2010. "Production of Bioactive Compounds from Fungi Grown on Ginseng-Steaming Effluent" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 38(2): 129~135     
    4. Jang, In-Taek ; Kim, Young-Hun ; Yi, Sung-Hun ; Lim, Sung-Il ; Lee, Jong-Soo 2011. "Screening of a New Fibrinolytic Substances-Producing Yeast" 한국균학회지 = The Korean journal of mycology, 39(3): 227~228     
    5. Baek, Seung-Ye ; Nam, Yun-Gyu ; Ju, Jung-Il ; Lee, Jong-Soo 2011. "Changes of Quality Characteristics during Storage of Gugija-Liriope tuber Makgeolli Made by Saccharomyces cerevisiae C-2" 한국균학회지 = The Korean journal of mycology, 39(2): 122~125     
    6. Jang, In-Taek ; Kim, Young-Hun ; Kang, Min-Gu ; Yi, Sung-Hun ; Lim, Sung-Il ; Lee, Jong-Soo 2012. "Production of Tyrosinase Inhibitor from Saccharomyces cerevisiae" 한국균학회지 = The Korean journal of mycology, 40(1): 60~64     
    7. Min, Jin Hong ; Lee, Hyang Burm ; Lee, Jong Soo ; Kim, Ha Kun 2013. "Identification of Yeasts Isolated from Wild Flowers Collected in Coast Areas of Korea Based on the 26S rDNA Sequences" 한국균학회지 = The Korean journal of mycology, 41(3): 185~191     
    8. 2014. "" 한국균학회지 = The Korean journal of mycology, 42(2): 170~173     
    9. Kim, Young-Hun ; Shin, Ja-Won ; Lee, Jong-Soo 2014. "Production and Anti-hyperglycemic Effects of ${\alpha}$-Glucosidase Inhibitor from Yeast, Pichia burtonii Y257-7" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 42(3): 219~224     
    10. Han, Sang-Min ; Hyun, Se-Hee ; Lee, Jong-Soo 2015. "Isolation and Identification of Yeasts from Wild Flowers in Deogyu Mountain and their Physiological Functionalities" 한국균학회지 = The Korean journal of mycology, 43(1): 47~52     
    11. Han, Sang-Min ; Park, Won-Jong ; Lee, Jong-Soo 2015. "Isolation and Diversity of Wild Yeasts from Some Cereals" 한국균학회지 = The Korean journal of mycology, 43(1): 64~67     
    12. Han, Sang-Min ; Hyun, Se-Hee ; Kim, Na-Mi ; Lee, Jong-Soo 2015. "Antioxidant Activity and Inhibitory Activities of Xanthine Oxidase and Tyrosinase of Yeasts from Wild Flowers in Korea" 한국균학회지 = The Korean journal of mycology, 43(2): 99~103     
    13. Hyun, Se-Hee ; Han, Sang-Min ; Kim, Ha-Kun ; Lee, Jong-Soo 2015. "Yeasts Diversity of Wild Flowers in Mountains of Korea and Their Physiological Functionalities" 한국균학회지 = The Korean journal of mycology, 43(3): 137~141     
    14. Han, Sang-Min ; Kim, Ha-Kun ; Lee, Hyang-Burm ; Lee, Jong-Soo 2016. "Isolation and Identification of Wild Yeasts from Freshwaters and Soils of Nakdong and Yeongsan River, Korea, with Characterization of Two Unrecorded Yeasts" 한국균학회지 = The Korean journal of mycology, 44(4): 350~354     
    15. Han, Sang-Min ; Han, Jae-Won ; Bae, Sang-Min ; Park, Won-Jong ; Lee, Jong-Soo 2016. "Isolation and Identification of Wild Yeasts from Soils of Fields in Daejeon Metropolitan City and Chungcheongnam-do, Korea" 한국균학회지 = The Korean journal of mycology, 44(1): 1~7     

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