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Journal of microbiology and biotechnology v.14 no.6, 2004년, pp.1343 - 1349   피인용횟수: 1
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Molecular Cloning and Characterization of the Gene for Outer Membrane Protein H in a Pasteurella multocida (D:4) Isolate from Pigs with Atrophic Rhinitis Symptoms in Korea

LEE, JEONG-MIN    (The Institute of Life Science and Technology, Sungkyunkwan University   ); KANG, SEO-YOUNG    (Department of Genetic Engineering, Sungkyunkwan University   ); PARK, SHIN-IN    (Department of Food and Nutrition, Kyungwon University   ); WOO, HEE-JONG    (College of Veterinary Medicine, Seoul National University   ); KWON, MOO-SIK    (Department of Genetic Engineering, Sungkyunkwan University  );
  • 초록

    A native strain of Pasteurella multocida was isolated from pigs suffering from severe atrophic rhinitis at domestic farms in Gyeonggi Province, Korea, and was identified as capsular serogroup 'D' and somatic serotype '4' by disc diffusion decapsulation and gel diffusion precipitation tests, respectively. The P. multocida (D:4) induced atrophic rhinitis in healthy pigs by the secondary infection. The gene for outer membrane protein H (ompH) of P. multocida (D:4) was cloned in Escherichia coli DH5 $\alpha$ by PCR. The open reading frame of the ompH was composed of 1,023 bp, possibly encoding a protein with 341 amino acid residues containing a signal peptide of 20 amino acids at N-terminus, and the gene product with molecular mass of ca. 38 kDa was identified by SDS-PAGE. Hydropathy profiles indicated that there are two variable domains in the OmpH. To express the ompH in E. coli, the gene was manipulated in various ways. Expression of the truncated as well as full-length forms of the recombinant OmpH was fatal to the host E. coli BL21 (DE3). However, the truncated OmpH fused with GST was consecutively expressed in E. coli DH5 $\alpha$ . A large quantity of the fused polypeptide was purified through GST-affinity chromatography.

  • 주제어

    Pasteurella multocida (D:4) .   pig atrophic rhinitis .   bacterial outer-membrane proteins .   outer-membrane protein H (OmpH).  

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    1. 2010. "" Journal of microbiology and biotechnology, 20(12): 1756~1763     

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