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Macromolecular research v.12 no.6, 2004년, pp.586 - 592  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Radiolytic Immobilization of Lipase on Poly(glycidyl methacrylate )-grafted Polyethylene Microbeads

Choi Seong-Ho    (Department of Chemistry, Hannam University   ); Lee Kwang-Pill    (Department of Chemistry Graduate School, Kyungpook National University   ); Kang Hee-Dong    (Department of Physics, Kyungpook National University   ); Park Hyun Gyu    (Department of Chemical & Biomolecular Engineering, Korea Advanced Institute of Science & Technology (KAIS)  );
  • 초록

    Poly(glycidyl methacrylate)-grafted polyethylene microbeads (POPM) presenting epoxy groups were prepared by radiation-induced graft polymerization of glycidyl methacrylate on the polyethylene microbead. The obtained POPM was characterized by IR spectroscopic, X-ray photoelectrons spectroscopy (XPS), scanning electron microscope (SEM), and thermal analyses. Furthermore, the abundance of epoxy groups on the POPM was determined by titration and elemental analysis after amination. The epoxy group content was calculated to be in the range 0.29-0.34 mmol/g when using the titration method, but in the range 0.53-0.59 mmol./g when using elemental analysis (EA) after amination. The lipase was immobilized to the epoxy groups of the POPM under various experi­mental conditions, including changes to the pH and the epoxy group content. The activity of the lipase-immobilized POPM was in the range from 160 to 500 unit/mg-min. The activity of the lipase-immobilized POPM increased upon increasing the epoxy group content. The lipase-immobilized POPM was characterized additionally by SEM, elec­tron spectroscopy for chemical analysis (ESCA), and EA.


  • 주제어

    epoxy group .   glycidyl methacrylate .   immobilization .   lipase .   lipase activity .   poly(glycidyl methacrylate) .   radiation-induced graft polymerization.  

  • 참고문헌 (22)

    1. W. F. Gum, W. Riese, and H.I. Ulbrich, Reactious Polymer, New York, Carl Hauser, 1992, pp 146-153 
    2. S.-H. Choi and Y. C. Nho, J. Appl. Polym. Sci., 71 , 2227 (1999) 
    3. S.-H. Choi and Y. C. Nho, Radiati. Phys. Chem., 57, 187 (2000) 
    4. S.-H. Choi, K.-P. Lee, J.-G. Lee, and Y. C. Nho, J. Appl Polym. Sci., 77, 500 (2000) 
    5. S.-H. Choi, K.-P. Lee, and Y. C. Nho, KOT Polym. J., 7, 297 (1999) 
    6. S.-H. Choi, H.-J. Kang, E.-N. Ryu, and K.-P. Lee, Radiati. Phys. Chem., 60, 503 (2001) 
    7. S. Devi and P. Sridhar, Process Biochem., 36, 225 (2000) 
    8. S.-H. Choi, M.-S. Kim, J. J. Ryoo, K.-P. Lee, H.-D. Shin, S.H. Kim, and Y.-H. Lee, J. Appl. Polym. Sci., 85, 2451 (2002) 
    9. S.-H. Choi, K.-P. Lee, and Y. C. Nho, J. Appl. Polym. Sci., 80, 2851 (2001) 
    10. M. Landt, S. C. Boltz, and L. G. Butler, Biochemistry, 17, 915 (1978) 
    11. S.-H. Choi, K.-P. Lee, and H. D. Kang, J. Appl. Polym. Sci., 88, 1153 (2003) 
    12. A. Albeck and S. Kliper, Biochem. J., 346 71 (2000) 
    13. B.-D. Yin, Y.-CO Chen, S.-CO Lin, and W.-H. Hsu, Process Biochem., 35, 915 (2000) 
    14. J. Okamoto, T. Sugo, A. Katakai, and H. Omichi, J. Appl. Polym. Sci., 30, 2967 (1985) 
    15. S.-H. Choi, Y.-H. Jeong, J. J. Ryoo, and K.-P. Lee, Radiati. Phys. Chem., 60, 503 (2001) 
    16. B. Chakravorty, J. Membr. Sci., 41, 155 (1989) 
    17. K.-P. Lee, H. J. Kang, D. R. Joo, and S.-H. Choi, Radiati. Phys. Chem., 60, 473 (2001) 
    18. S.-H. Choi and Y. C. Nho, J. Appl. Polym. Sci., 71, 2227 (1999) 
    19. S.-H. Choi, G.-T. Kim, and Y. C. Nho, J. Appl. Polym. Sci., 71, 643 (1999) 
    20. M. Yoshida, M. Asano, and T. Yokota, J. Polym. Sci.; Part C: Polym. Lett., 27, 437 (1989) 
    21. A. L. Paiva, V. M. Balcao, and F. X. MaIcata, Enzyme Microb. Technol., 27,187 (2000) 
    22. G. M. Kline, Analytical Chemistry of Polymers, Interscience, New York, 1959 

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  • Lee Kwang-Pill (1)

  • Park Hyun Gyu (0)

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