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The journal of microbiology v.42 no.4, 2004년, pp.365 - 368  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Occurrence of the strA-strB Streptomycin Resistance Genes in Pseudomonas Species Isolated from Kiwifruit Plants

Han Hyo Shim    (Department of Biology, Sunchon National University   ); Koh Young Jin    (Department of Applied Biology, Sunchon National University   ); Hur Jae-Seoun    (Department of Environmental Education, Sunchon National University   ); Jung Jae Sung    (Department of Biology, Sunchon National University  );
  • 초록

    The occurrence of strA-strB streptomycin-resistance genes within transposon Tn5393 was examined in Pseudomonas syringae pv. actinidiae, P. syringae pv. syringae, and P. marginalis, isolated from kiwifruit plants in Korea and Japan. PCR amplification with primers specific to strA-strB revealed that three of the tested Pseudomonas species harbored these genes for a streptomycin-resistance determinant. Tn5393, containing strA-strB, was also identified with PCR primers designed to amplify parts of tnpA, res, and tnpR. No IS elements were detected within tnpR, nor were they found in the intergenic region between tnpR and strA. Nucleotide sequence analysis indicated that the strA sequence of P. syringae pv. actinidiae contained a single nucleotide alteration at position 593 (CAA $\rightarrow$ CGA), as compared to Tn5393a in P. syringae pv. syringae. This resulted in an amino acid change, from Gin to Arg.


  • 주제어

    Pseudomonas syringae pv.   syringae .   kiwifruit .   strA-strB .   Tn5393.  

  • 참고문헌 (11)

    1. Lioa, C.H., D.E. McCallus, W.F. Fett, and Y. Yang. 1997. Identification of gene loci controlling pectate lyase production and soft-rot pathogenicity in Pseudomonas marginalis. Can. J. Microbiol. 43, 425-431 
    2. Han, H.S., Y.J. Koh, J.-S. Hur, and J.S. Jung. 2003b. Identification and characterization of coronatine-producing Pseudomonas syringae pv. actinidiae. J. Microbiol. Biotechnol. 13, 110-118 
    3. Sundin, G.W. 2002. Distinct recent lineages of the strA-strB streptomycin-resistance genes in clinical and environmental bacteria. Curr. Microbiol. 45, 63-69 
    4. Chiou, C.-S. and A.L. Jones. 1993. Nucleotide sequence analysis of a transposon (Tn5393) carrying streptomycin resistance genes in Erwinia amylovora and other Gram-negative bacteria. J. Bacteriol. 175, 732-740 
    5. Sundin, G.W. and C.L. Bender. 1996. Dissemination of the strAstrB streptomycin-resistance genes among commensal and pathogenic bacteria from humans, animals, and plants. Mol. Ecol. 5,133-143 
    6. Burr, T.J., J.L. Norelli, and C.L. Reid. 1993. Streptomycin-resistant bacteria associated with fire blight infections. Plant Dis. 77, 63-66 
    7. Balestra, G.M. and L. Varvaro. 1997. Pseudomonas syringae pv. syringae causative agent of disease on floral buds of Actinidia deliciosa (A. Chev) Liang et Ferguson in Italy. J. Phytopathology 145, 375-378 
    8. Shaw, K.J., P.N. Rather, R.S. Hare, and G.H. Miller. 1993. Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol. Rev. 57, 138-163 
    9. LAbee-Lund, T.M. and H. Sørum. 2000. Functional Tn5393-like transposon in the R plasmid pRAS2 from the fish pathogen Aeromonas salmonicida subspecies salmonicida isolated in Norway. Appl. Environ. Microbiol. 66, 5533-5535 
    10. Han, H.S., H.Y. Nam, Y.J. Koh, J.-S. Hur, and J.S. Jung. 2003a. Molecular bases of high-level streptomycin resistance in Pseudomonas marginalis and Pseudomonas syringae pv. actinidiae. J. Microbiol. 41, 16-21 
    11. Sundin, G.W. and C.L. Bender. 1995. Expression of the strA-strB streptomycin resistance gene in Pseudomonas syringae and Xanthomonas campestris and characterization of IS6100 in X. campestris. Appl. Environ. Microbiol. 61, 2891-2897 

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