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Korean journal of crop science = 韓國作物學會誌 v.49 no.5, 2004년, pp.415 - 418  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Effects of Sonication and Vacuum Infiltration on Agrobacterium-Mediated Transformation in Immature Embryos of Korean Wheat Genotypes

Moon Jung-Hun    (Dept. of Plant Resources, Dongguk Univ.   ); Kang Moon-Suk    (National Institute of Crop Science, RDA   ); Heo Hwa-Young    (National Institute of Crop Science, RDA   ); Kwon Young-Up    (National Institute of Crop Science, RDA   ); Lee Sang-Kyu    (Dept. of Plant Resources, Dongguk Univ.   ); Lee Kyung-Hee    (Dept. of Plant Resources, Dongguk Univ.   ); Lee Byung-Moo    (Dept. of Plant Resources, Dongguk Univ.  );
  • 초록

    The effects of sonication and vacuum infiltration on transformation efficiency was investigated by using immature embryos of Korean wheat as explants. Two Agrobacterium tumefaciens strains, KYRT1 and EHA105, carrying pCAMBIA 1305.1 were used. Transformation efficiency was demonstrated by the detection of $\beta-glucu-ronidase$ (GUS) activity. GUS expression showed clear difference among Korean wheat cultivars. Geurumil showed higher GUS expression efficiency $79.1\%$ compared with other cultivars. The effects of the duration of vacuum infiltration and sonication treatment showed a tendency high GUS expression efficiency by their combination. In comparison with other Agrobacterium strains, KYRT1 showed high efficiency in most Korean cultivars.


  • 주제어

    Korean wheat cultivar .   Agrobacterium tumefaciens .   transformation .   vacuum infiltration .   sonication.  

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