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Inhibition of Interleukin-12 Production in Mouse Macrophages via Decreased Nuclear $Factor-\kappaB$ DNA Binding Activity by Myricetin, a Naturally Occurring Flavonoid

Kang Bok Yun    (College of Pharmacy and Research Institute of Drug Development, Chonnam National University   ); Kim Seung Hyun    (College of Pharmacy and Research Institute of Drug Development, Chonnam National University   ); Cho Dae Ho    (Department of Life Science, Sookmyung Womens University   ); Kim Tae Sung    (College of Pharmacy and Research Institute of Drug Development, Chonnam National University  );
  • 초록

    Pharmacological inhibition of interleukin-12 (IL-12) production may be a therapeutic strategy for preventing the development and progression of disease in experimental models of autoimmunity. In this study, the effects of myricetin, a naturally occurring flavonoid present in fruits, vegetables and medicinal herbs, on the production of IL-12 were investigated in mouse macrophages stimulated with lipopolysaccharide (LPS). Myricetin significantly inhibited the LPS­induced IL-12 production from both primary macrophages and the RAW264.7 monocytic cell-line in a dose-dependent manner. The effect of myricetin on IL-12 gene promoter activation was analyzed by transfecting RAW264.7 cells with IL-12 gene promoter/luciferase constructs. The repressive effect was mapped to a region in the IL-12 gene promoter containing a binding site for NF- ${\kappa}B$ . Furthermore, activation of macrophages by LPS resulted in markedly enhanced binding activity to the NF- ${\kappa}B$ site, which significantly decreased upon addition of myricetin, indicating that myricetin inhibited IL-12 production in LPS-activated macrophages via the down­regulation of NF-KB binding activity.


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  • 이 논문을 인용한 문헌 (2)

    1. 2006. "" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology, 14(1): 11~18     
    2. 2007. "" Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea, 30(9): 1075~1079     

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