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Comparison of Spectrophotometric, HPLC and Chemilumines­cence Methods for 3-Nitrotyrosine and Peroxynitrite Interaction

Turan Nilufer Nermin    (Gazi University, Faculty of Pharmacy, Department of Pharmacology   ); Ark Mustafa    (University of Mersin, Faculty of Medicine, Department of Pharmacology   ); Demiryurek Abdullah Tuncay    (University of Gaziantep, Faculty of Medicine, Department of Pharmacology  );
  • 초록

    We have studied the interaction of 3-nitrotyrosine with peroxynitrite using three different methods; chemiluminescence, spectrophotometry and HPLC. Peroxynitrite-induced luminol or lucigenin chemiluminescence were significantly decreased by 3-nitrotyrosine, in concentration­dependent manners. The intensity of the peroxynitrite spectrum was also markedly reduced in the presence of 3-nitrotyrosine in the spectrophometric assay. However, there was no attenuation of the 3-nitrotyrosine signal in the HPLC assay after mixing with peroxynitrite. The interaction of 3-nitrotyrosine and hypochlorous acid (HOCI) was also studied via the chemilumines-cence assay, where the HOCI-induced responses were markedly inhibited by 3-nitrotyrosine. These results suggest that caution should be taken when studying the levels or interactions of 3-nitrotyrosine.


  • 참고문헌 (34)

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