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The journal of microbiology v.43 no.2, 2005년, pp.179 - 182  

Enhancement of Gene Delivery to Cancer Cells by a Retargeted Adenovirus

Oh Kwang Seok    (Department of Biology, Hanseo University   ); Engler Jeffrey A.    (Departments of Biochemistry and Molecular Genetics, Schools of Medicine and Dentistry, University of Alabama at Birmingham   ); Joung In Sil    (Department of Biology, Hanseo University  );
  • 초록

    The inefficiency of in vivo gene transfer using currently available vectors reflects a major hurdle in cancer gene therapy. Both viral and non-viral approaches that improve gene transfer efficiency have been described, but suffer from a number of limitations. Herein, a fiber-modified adenovirus, carrying the small peptide ligand on the capsid, was tested for the delivery of a transgene to cancer cells. The fiber-modified adenovirus was able to mediate the entry and expression of a $\beta$ -galactosidase into cancer cells with increased efficiency compared to the unmodified adenovirus. Particularly, the gene transfer efficiency was improved up to 5 times in OVCAR3 cells, an ovarian cancer cell line. Such transduction systems hold promise for delivering genes to transferrin receptor overexpressing cancer cells, and could be used for future cancer gene therapy.


  • 참고문헌 (15)

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