본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

The journal of microbiology v.43 no.2, 2005년, pp.204 - 208   피인용횟수: 5

Identification of Non-mutans Streptococci Organisms in Dental Plaques Recovering on Mitis-Salivarius Bacitracin Agar Medium

Yoo So Young    (Department of Oral Biochemistry, College of Dentistry, Chosun University   ); Kim Pyung Sik    (Department of Conservative Dentistry, College of Dentistry, Chosun University   ); Hwan Ho Keel    (Department of Conservative Dentistry, College of Dentistry, Chosun University   ); Lim Seong Hoon    (Department of Orthodontics, College of Dentistry, Chosun University   ); Kim Kwang Won    (Department of Orthodontics, College of Dentistry, Chosun University   ); Choe Son Jin    (Department of Oral Microbiology and Immunology, College of Dentistry, Seoul National University   ); Min Byung Moo    (Department of Oral Biochemistry, College of Dentistry, Seoul National University   ); Kook Joong Ki    (Department of Oral Biochemistry, College of Dentistry, Seoul National University  );
  • 초록

    The objective of this study was to both isolate and identify non-mutans streptococci organisms (non­MSO) from dental plaques recovered on mitis-salivarius sucrose bacitracin agar (MSB) plates. The dental plaque samples, which had been collected from 63 human subjects, were diluted and plated on MSB. The bacteria growing on the MSB plates were then identified with biochemical tests, as well as with 16S rDNA cloning and sequencing techniques. Our data indicated that bacteria from 30 subjects had been recovered on the MSB plates. Among the 21 typical colonies selected from the 30 subjects, 12 colonies, derived from 10 subjects, were identified as non-MSO. These 12 colonies were determined to be Streptococcus anginosus (8 colonies), S. sanguinis (1 colony), and Pantoea agglomerans (3 colonies). These results strongly suggest that a new selective medium will be required for the reliable isolation of mutans streptococci.


  • 참고문헌 (16)

    1. Kimmel, L. and N. Tinanoff. 1991. A modified mitis salivarius medium for a caries diagnostic test. Oral Microbiol. Immunol. 6, 275-279 
    2. Niven, Jr., C.F., K.L. Smiley, and J.M. Sherman. 1942. The hydrolysis of arginine by streptococci. J. Bacteriol. 43, 651-660 
    3. Tanzer, J.M., A.C. Borjesson, L. Laskowski, A.B. Kurasz, and M. Testa. 1984. Glucose-sucrose-potassium tellurite-bacitracin agar, an alternative to mitis salivarius-bacitracin agar for enumeration of Streptococcus mutans. J. Clin. Microbiol. 20, 653-659 
    4. Van Palenstein Helderman, W.H., M. Ijsseldijk, and J.H. Huis in 't Veld. 1983. A selective medium for the two major subgroups of the bacterium Streptococcus mutans isolated from human dental plaque and saliva. Arch. Oral Biol. 28, 599-603 
    5. Farmer III, J.J. 1999. Enterobacteriaceae: Introduction and identification, p. 442-458. In P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, and R.H. Yolken (eds.), Manual of clinical microbiology. American Society for Microbiology, Washington, D.C 
    6. Whiley, R.A. and D. Beighton. 1998. Current classification of the oral streptococci. Oral Microbiol. Immunol. 13, 195-216 
    7. Jensen, B. and D. Bratthall. 1989. A new method for the estimation of mutans streptococci in human saliva. J. Dent. Res. 68, 468-471 
    8. Hirasawa, M. and K. Takada. 2002. Susceptibility of Streptococcus mutans and Streptococcus sobrinus to cell wall inhibitors and development of a novel selective medium for S. sobrinus. Caries Res. 36, 155-160 
    9. Lane, D.J. 1991. 16S/23S rRNA sequencing, p. 115-175. In E. Stackebrandt, and M. Goodfellow (eds.), Nucleic acid techniques in bacterial systematics. John Wiley & Sons, Inc., New York 
    10. Loesche, W.J. 1986. Role of Streptococcus mutans in human dental decay. Microbiol. Rev. 50, 353-380 
    11. Shklair, IL. and H.J. Keene. 1976. Biochemical characterization and distribution of Streptococcus mutans in three diverse populations, p. 201-210. In H.M. Stiles, W.J. Loesche, and T.C. O'Brien (eds.), Microbial aspects of dental caries. Information Retrieval Inc., Washington, D.C 
    12. Emilson, C.G. 1983. Prevalence of Streptococcus mutans with different colonial morphologies in human plaque and saliva. Scand. J. Dent. Res. 91, 26-32 
    13. Krasse, B. 1966. Human streptococci and experimental caries in hamsters. Arch. Oral Biol. 11, 429-436 
    14. Hirasawa, M. and K. Takada. 2003. A new selective medium for Streptococcus mutans and the distribution of S. mutans and S. sobrinus and their serotypes in dental plaque. Caries Res. 37, 212-217 
    15. Shklair, I.L. and H.J. Keene. 1974. A biochemical scheme for the separation of the five varieties of Streptococcus mutans. Arch. Oral Biol. 19, 1079-1081 
    16. Gold, O.G., H.V. Jordan, and J. van Houte. 1973. A selective medium for the isolation of Streptococcus mutans. Arch. Oral Biol. 18, 1357-1364 
  • 이 논문을 인용한 문헌 (5)

    1. 2006. "" International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute, 31(1): 7~10     
    2. Choi, Min-Ho ; Yoo, So-Young ; Lim, Chae-Kwang ; Kang, Dong-Wan ; Kook, Joong-Ki 2006. "Nested PCR for the Detection of Streptococcus mutans" Korean journal of microbiology = 미생물학회지, 42(1): 19~25     
    3. 2007. "" The journal of microbiology, 45(3): 246~255     
    4. Jeong, Hyun-Ja ; Kim, Su-Jung 2010. "Distribution and Antimicrobial Susceptibility of Bacteria in the Oral Cavity of Smokers or Non-Smokers" Korean journal of microbiology = 미생물학회지, 46(4): 334~340     
    5. 2010. "" International journal of oral biology : official journal of the Korean Academy of Oral Biology and the UCLA Dental Research Institute, 35(1): 21~25     

 저자의 다른 논문

  • Yoo, So Young (22)

    1. 2003 "분자생물학적 기법을 이용한 악골 골수염 병소의 세균 동정" 大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons 29 (1): 48~55    
    2. 2003 "미반응 레진단량체가 우식유발성 세균의 활성에 미치는 영향" 大韓小兒齒科學會誌 = Journal of the Korean academy of pediatric dentistry 30 (4): 684~695    
    3. 2003 "각종 치과레이저의 Streptococcus mutans에 대한 증식 및 기능억제 효과" 大韓小兒齒科學會誌 = Journal of the Korean academy of pediatric dentistry 30 (3): 439~447    
    4. 2003 "녹차 결명자 추출물의 교정용 브라켓과 치면 사이의 경계부에서 분리된 mutans streptococci에 대한 항균작용" 대한치과교정학회지 = The Korean journal of orthodontics 33 (5): 381~389    
    5. 2003 "중합효소연쇄반응법을 이용한 급성 치수 및 치근단 질환의 병원성 세균의 동정" 大韓齒科保存學會誌 = Journal of Korean Academy of Operative Dentistry 28 (2): 178~183    
    6. 2003 "치주질환이 없는 청년의 치은연상 및 치은연하 치면세균막에 존재하는 치주질환 관련 4종 세균의 분포 비교" 대한치주과학회지 = The journal of Korean academy of periodontology 33 (2): 159~166    
    7. 2003 "Cefuroxime axetil의 치주병원성 세균에 대한 최소억제농도와 치은열구액 농도에 관한 실험적 연구" 대한치주과학회지 = The journal of Korean academy of periodontology 33 (3): 341~348    
    8. 2004 "소아의 치면세균막에 존재하는 mutans streptococci의 분포" 大韓小兒齒科學會誌 = Journal of the Korean academy of pediatric dentistry 31 (3): 439~447    
    9. 2004 "16S rDNA 클론 Libraries를 이용한 치근단 농양 병소의 세균 동정" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology 32 (2): 195~198    
    10. 2004 "Multiplex PCR Using Conserved and Species-Specific 16S rDNA Primers for Simultaneous Detection of Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans" Journal of microbiology and biotechnology 14 (1): 110~115    
  • 김광원 (65)

  • Choe, Son-Jin (21)

  • Min, Byung-Moo (14)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기