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The journal of microbiology v.43 no.2, 2005년, pp.213 - 218  

Molecular Taxonomy of a Soil Actinomycete Isolate, KCCM10454 Showing Neuroprotective Activity by 16S rRNA and rpoB Gene Analysis

Lee Bong Hee    (Department of Anatomy & Neurobiology, Cheju National University   ); Kim Hong    (Department of Microbiology, and Liver Research Institute, College of Medicine, Seoul National University   ); Kim Hyun Ju    (Department of Microbiology, and Liver Research Institute, College of Medicine, Seoul National University   ); Lim Yoon Kyu    (Department of Veterinary Public Health, College of Veterinary Medicine, Cheju National University   ); Byun Kyung Hee    (Department of Anatomy & Neurobiology, Cheju National University   ); Hutchinson Brian    (Department of Anatomy & Neurobiology, Cheju National University   ); Kim Chang Jin    (Korea Research institute of Bioscience and Biotechnology   ); Ko Young Hwan    (Department of Food Science and Engineering, Cheju National University   ); Lee Keun Hwa    (Department of Microbiology, College of Medicine, Institute of Medical Science, Cheju National University   ); Cha Chang Yong    (Department of Microbiology, and Liver Research Institute, College of Medicine, Seoul National University   ); Kook Yoon Hoh    (Department of Microbiology, and Liver Research Institute, College of Medicine, Seoul National University   ); Kim Bum Joon    (Department of Microbiology, and Live  );
  • 초록

    Epilepsy constitutes a significant public health problem, and even the newest drugs and neurosurgical techniques have proven unable to cure the disease. In order to select a group of isolates which could generate an active compound with neuroprotective or antiepileptic properties, we isolated 517 actinomycete strains from soil samples taken from Jeju Island, in South Korea. We then screened these strains for possible anti-apoptotic effects against serum deprivation-induced hippocampal cell death, using the 3-(4, 5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay as an in vitro test. The excitotoxic glutamate analog, kainic acid (KA), was used to induce seizures in experimental mice in our in vivo tests. As a result of this testing, we located one strain which exhibited profound neuroprotective activity. This strain was identified as a Streptomyces species, and exhibited the rifampinresistant genotype, Asn $(AAC)^$ 442, according to the results of 16S rRNA and rpoB gene analyses


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