본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.15 no.2, 2005년, pp.259 - 264   피인용횟수: 1

Use of Neonatal Chondrocytes for Cartilage Tissue Engineering

KANG SUN WOONG    (Department of Chemical Engineering, Hanyang University   ); PARK JUNG HO    (Department of Orthopeadic Surgery, Ansan Hospital, College of Medicine, Korea University   ); KIM BYUNG SOO    (Department of Chemical Engineering, Hanyang University  );
  • 초록

    Transplantation of cultured chondrocytes can regenerate cartilage tissues in cartilage defects in humans. However, this method requires a long culture period to expand chondrocytes to a large number of cells for transplantation. In addition, chondrocytes may dedifferentiate during long-term culture. These problems can potentially be overcome by the use of undifferentiated or partially developed cartilage precursor cells derived from neonatal cartilage, which, unlike chondrocytes from adult cartilage, have the capacity for rapid in vitro cell expansion and may retain their differentiated phenotype during long-term culture. The purpose of this study was to compare the cell growth rate and phenotypic modulation during in vitro culture between adult chondrocytes and neonatal chondrocytes, and to demonstrate the feasibility of regenerating cartilage tissues in vivo by transplantation of neonatal chondrocytes expanded in vitro and seeded onto polymer scaffolds. When cultured in vitro, chondrocytes isolated from neonatal (immediately postpartum, 2 h of age) rats exhibited much higher growth rate than chondrocytes isolated from adult rats. After 5 days of culture, more neonatal chondrocytes were in the differentiated state than adult chondrocytes. Cultured neonatal chondrocytes were seeded onto biodegradable polymer scaffolds and transplanted into athymic mice's subcutaneous sites. Four weeks after implantation, neonatal chondrocyte-seeded scaffolds formed white cartilaginous tissues. Histological analysis of the implants with hematoxylin and eosin showed mature and well-formed cartilage. Alcian blue/ safranin-O staining and Masson's trichrome staining indicated the presence of highly sulfated glycosarninoglycans and collagen, respectively, both of which are the major extracellular matrices of cartilage. Immunohistochemical analysis showed that the collagen was mainly type II, the major collagen type in cartilage. These results showed that neonatal chondrocytes have potential to be a cell source for cartilage tissue engineering.

  • 참고문헌 (22)

    1. Meyers, M. H., W Akeson, and F. R. Convery. 1989. Resurfacing of the knee with fresh osteochondral allograft. J. Bone Joint Surg. Am. 71: 704- 713 
    2. Morihara, T., F. Harwood, R. Goerner, Y. Hirasawa, and D. Amiel. 2002. Tissue-engineered repair of osteochondral defects: Effects of the age of donor cells and host tissue. Tissue Eng. 8: 921- 929 
    3. Rotter, N., L. J. Bonassar, G. Tobias, M. Lebl, A. K. Roy, and C. A. Vacanti. 2002. Age dependence of biochemical and biomechanical properties of tissue-engineered human septal cartilage. Biomaterials 23: 3087- 3094 
    4. Brittberg, M., A. Lindahl, A. Nilsson, C. Ohlsson, O. Isaksson, and L. Peterson. 1994. Treatment of deep cartilage defects in knee with autologous chondrocyte transplantation. N. Engl. J. Med. 331: 889- 895 
    5. Chung, M. I., M. H. Lim, Y. J. Lee, I. H. Kim, I. Y. Kim, J. H. Kim, K. Chang, and H. Kim. 2003. Reduction of ammonia accumulation and improvement of cell viability by expression of urea cycle enzymes in Chinese hamster ovary cells. J. Microbiol. Biotechnol. 13: 217 - 224 
    6. Fuchs, J. R., S. Terada, D. Hannouche, E. R. Ochoa, J. P. Vacanti, and D. O. Fauza, 2003. Fetal tissue engineering: Chest wall reconstruction. J. Pediatr. Surg. 38: 1188- 1193 
    7. Na, K. and K. Park. 2004. Immobilization of Arg-Gly-Asp (RGD) sequence in sugar-containing copolymer for culturing fibroblast cells. J. Microbiol. Biotechnol. 14: 193- 196 
    8. Veilleux, N. H., I. V. Yannas, and M. Spector. 2004. Effect of passage number and collagen type on the proliferative, biosynthetic, and contractile activity of adult canine articular chondrocytes in type I and II collagen-glycosaminoglycan matrices in vitro. Tissue Eng. 10: 119- 127 
    9. von der Mark, K., V. Gauss, H. von der Mark, and P. Muller. 1977. Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture. Nature 9: 531- 532 
    10. Minas, T. and S. Nehrer. 1997. Current concepts in the treatment of articular cartilage defects. Orthopedics 20: 525- 538 
    11. Benya, P. D. and J. D. Shaffer. 1982. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30: 215- 224 
    12. Dozin, B., M. Malpeli, L. Camardella, R. Cancedda, and A. Pietrangelo. 2002. Response of young, aged and osteoarthritic human articular cnondrocytes to inflammatory cytokines: Molecular and cellular aspects. Matrix Biology 21: 449-459 
    13. Convery, F. R., W. H. Akeson, D. Amiel, M. H. Meyers, and A. Monosov, 1996. Long-term survival of chondrocytes in an osteochondral articular cartilage allograft. A case report. J. Bone Joint Surg. Am. 78: 1082- 1088 
    14. Kim, B., S. I. Jeong, S. Cho, J. Nikolovski, D. J. Mooney, S. H. Lee, O. Jeon, T. W. Kim, S. H. Lim, Y. S. Hong, C. Y. Choi, Y. M. Lee, S. H. Kim, and Y. H. Kim. 2003. Tissue engineering of smooth muscle under a mechanically dynamic condition. J. Microbiol. Biotechnol. 13: 841- 845 
    15. Dominice, J., C. Levasseur, S. Larno, X. Ronot, and M. Adolphe. 1986. Age-related changes in rabbit articular chondrocytes, Mech. Ageing Dev. 37: 231- 240 
    16. Dekel, B., T. Burakova, F. D. Arditti, S. Reich-Zeliger, O. Milstein, S. Aviel-Ronen, G. Rechavi, N. Friedman, N. Kaminski, J. H. Passwell, and Y. Reisner. 2003. Human and porcine early kidney precursors as a new source for transplantation. Nat. Med. 9: 53- 60 
    17. Ghazavi, M. T., K. P. Pritzker, A. M. Davis, and A. E. Gross. 1997. Fresh osteochondral allografts for post-traumatic osteochondral defects of the knee. J. Bone Joint Surg, Br. 79: 1008- 1013 
    18. Puelacher, W. C., S. W. Kim, J. P. Vacanti, B. Schloo, D. Mooney, and C. A. Vacanti. 1994. Tissue-engineered growth of cartilage: The effect of varying the concentration of chondrocytes seeded onto synthetic polymer matrices. J. Oral Maxillofac. Surg. 23: 49- 53 
    19. Yang, B., S. Jeong, and C. Song. 2002. Hypolipidemic effect of exo- and endo-biopolymers produced from submerged mycelial culture of Ganoderma lucidum in rats. J. Microbiol. Biotechnol. 12: 872- 877 
    20. Benya, P. D., S. R Padilla, and M. E. Nimni. 1978. Independent regulation of collagen types by chondrocytes during the loss of differentiated function in culture. Cell 15: 1313- 1321 
    21. Buckwalter, J. A. and H. J. Mankin. 1998. Articular cartilage: Degeneration and osteoarthritis, repair, regeneration, and transplantation. lnstr. Course Lect. 47: 487- 504 
    22. Mayne, R., M. S. Vail, P. M. Mayne, and E. J. Miller. 1976. Changes in type of collagen synthesized as clones of chick chondrocytes grow and eventually lose division capacity. Proc, Natl. Acad. Sci. USA 73: 1674- 1678 
  • 이 논문을 인용한 문헌 (1)

    1. 2006. "" Journal of microbiology and biotechnology, 16(10): 1577~1582     

 저자의 다른 논문

  • KIM, BYUNG-SOO (14)

    1. 1998 "철질산염을 이용한 $\alpha-Fe_2O_3$ 미세 분말 제조" 한국결정성장학회지 = Journal of the Korean crystal growth and crystal technology 8 (1): 138~144    
    2. 1998 "시화지구의 대기오염물질 확산에 관한 전산모사" 환경영향평가 = Journal of environmental impact assessment 7 (1): 35~48    
    3. 1999 "로울러 킬른 내의 이동현상에 관한 전산모사" 한국결정성장학회지 = Journal of the Korean crystal growth and crystal technology 9 (2): 251~259    
    4. 1999 "가습된 질소 분위기에서의 Kovar(Fe-29Ni-17Co)산화" 요업학회지 = Journal of the Korean Ceramic Society 36 (11): 1228~1234    
    5. 2001 "가습된 $N_2$/H$_2$혼합가스 분위기에서의 Kovar 산화 거동" 마이크로전자 및 패키징 학회지 = Journal of the Microelectronics and Packaging Society 8 (2): 1~7    
    6. 2001 "pH/온도 민감성 N-Isopropylacrylamide계 공중합체의 LCST거동에 대한 산성 공단량체의 구조적 영향" 폴리머 = Polymer (Korea) 25 (2): 186~198    
    7. 2003 "Tissue Engineering of Smooth Muscle under a Mechanically Dynamic Condition" Journal of microbiology and biotechnology 13 (6): 841~845    
    8. 2004 "BDNF 유전자 이입 슈반세포와 PGA 도관을 이용한 백서 좌골신경 재생에 관한 연구" 大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons 30 (6): 465~473    
    9. 2004 "조직공학적 인조혈관의 생체 내 이식 실험" 大韓胸部外科學會誌 = The Korean journal of thoracic and cardiovascular surgery 37 (3): 220~227    
    10. 2004 "Comparative Study of Seeding and Culture Methods to Vascular Smooth Muscle Cells on Biodegradable Scaffold" Journal of microbiology and biotechnology 14 (4): 707~714    

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역



유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기