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Journal of microbiology and biotechnology v.15 no.2, 2005년, pp.274 - 280   피인용횟수: 4

Purification and Characterization of Chitinase from Paenibacillus illinoisensis KJA-424

JUNG WOO JIN    (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University   ); KUK JU HEE    (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University   ); KIM KIL YONG    (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University   ); KIM TAE HWAN    (Department of Animal Science, College of Agriculture and Life Science, Chonnam National University   ); PARK RO DONG    (Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology Institute of Agricultural Science and Technology, Chonnam National University  );
  • 초록

    A chitinase was purified from the culture supernatant of Paenibacillus illinoisensis KJA-424 by protein precipitation, DEAE-Sephadex anion-exchange chromatography, and Sephadex G-150 gel filtration. The molecular weight of the purified chitinase was 54 kDa on SDS-PAGE and activity staining. Optimal pH and temperature were pH 5.0 and 60 $^{circ}$ C, the presence of 10 ruM Ag $^{+}$ and Hg $^{2+}$ inhibited the activity by $92.1/%$ and $97.7/%$ , and the K $_{m}$ and V $_{max}$ values were 1.12 mg chitin mrl and 1.48 $\mu$ mol GlcNAc min $^{-1}$ , respectively. The enzyme hydrolyzed tetramer to dimer, pentamer to dimer and trimer, and hexamer to dimer, trimer and tetramer, indicating an endo-splitting mechanism. The chitinase had no hydrolytic activity toward dimer and trimer. The chitinase inhibited the mycelial growth of Rhizoctonia solani, suggesting an antifungal property.


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