본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.15 no.2, 2005년, pp.346 - 353   피인용횟수: 9

Genetic Organization of a 50-kb Gene Cluster Isolated from Streptomyces kanamyceticus for Kanamycin Biosynthesis and Characterization of Kanamycin Acetyltransferase

ZHAO XIN QING    (Department of Biological Science and Institute of Bio-Science and Biotechnology, Myong Ji University   ); KIM KYOUNG ROK    (Department of Biological Science and Institute of Bio-Science and Biotechnology, Myong Ji University   ); SANG LI WEI    (Department of Biological Science and Institute of Bio-Science and Biotechnology, Myong Ji University   ); KANG SUK HO    (Department of Biological Science and Institute of Bio-Science and Biotechnology, Myong Ji University   ); YANG YOUNG YELL    (Department of Biological Science and Institute of Bio-Science and Biotechnology, Myong Ji University   ); SUH JOO WON    (Department of Biological Science and Institute of Bio-Science and Biotechnology, Myong Ji University  );
  • 초록

    A 50-kb chromosome DNA region was isolated from Streptomyces kanamyceticus by screening the fosmid genomic library, using the 16S rRNA methylase gene (kmr) as a probe. Sequence analysis of this region revealed 42 putative open reading frames (ORFs), which included biosynthetic genes such as genes responsible for 2-deoxystreptamine (2­DOS) biosynthesis as well as genes for resistance and regulatory function. Also, the kanamycin acetyltransferase gene (kac) was characterized by in vitro enzyme assay, which conferred E. coli BL21 (DE3) with 10, 50, and 80-times higher resistance to kanamycin A, tobramycin, and amikacin, respectively, than the control strain had, thus strongly indicating that the isolated gene cluster is very likely involved in kanamycin biosynthesis. This work provides a solid basis for further elucidation of the kanamycin biosynthesis pathway as well as the productivity improvement and construction of new hybrid antibiotics.


  • 참고문헌 (32)

    1. Ahlert, J., J. Distler, K. Mansouri, and W. Piepersberg. 1997. Identification of stsC, the gene encoding the L-glutamine:scyllo-inosose aminotransferase from Streptomycinproducing Streptomycetes. Arch. Microbiol. 168: 102- 113 
    2. Demydchuk, J., Z. Oliynyk, and V. Fedorenko. 1998. Analysis of a kanamycin resistance gene (kmr) from Streptomyces kanamvceticus and a mutant with increased arninoglycoside resistance. J. Basic Microbiol. 38: 231- 239 
    3. Distler, J., A. Ebert, K. Mansouri, K. Pissowotzki, M. Stockmann, and W. Piepersberg. 1987. Gene cluster for streptomycin biosynthesis in Streptomyces griseus: Nucleotide sequence of three genes and analysis of transcriptional activity. Nucl. Acids Res. 15: 8041- 8056 
    4. He, X. M. and H.W. Liu. 2002. Formation of unusual sugars: Mechanistic studies and biosynthetic applications. Annu. Rev. Biochem. 71: 701- 754 
    5. Magnet, S., T. A. Smith, R. J. Zheng, P. Nordmann, and J. S. Blanchard. 2003. Aminoglycoside resistance resulting from tight drug binding to an altered arninoglycoside acetyltransferase. Antimicrob. Agents Chemother. 47: 1577-1583 
    6. Nedal, A. and S. B. Zotchev. 2004. Biosynthesis of deoxyaminosugars in antibiotic-producing bacteria. Appl. Microbiol, Biotechnol. 64: 7- 15 
    7. Okuda, T. and Y. Ito. 1982. Biosynthesis and mutasynthesis of aminoglycoside antibiotics, pp. 154- 159. In H. Umezawa and I. R. Hooper (eds.), Aminoglycoside Antibiotics. SpringerVerlag, Berlin, Heidelberg, New York, U.S.A 
    8. Ikeno, S., D. Aoki, M. Hamada, M. Hori, and K. S. Tsuchiya. 2002. kasT gene of Streptomyces kasugaensis M38-M1 encodes a DNA-binding protein which binds to intergenic region of kasU-kasJ in the kasugarnycin biosynthesis gene cluster. J. Antibiotics 5: 1053- 1062 
    9. Distler, J., K. Mansouri, G. Mayer, M. Stockmann, and W. Piepersberg. 1992. Streptomycin biosynthesis and its regulation in Streptomycetes. Gene 115: 105-111 
    10. Shaw, K. J., P. N. Rather, R. S. Hare, and G. G. Miller. 1993. Molecular genetics of arninoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol. Rev. 57: 138- 16 
    11. Huang, F. L., Y. Y. Li, J. Q. Yu, and J. B. Spencer. 2002. Biosynthesis of aminoglycoside antibiotics: Cloning, expression and characterization of an aminotransferase involved in the pathway to 2-deoxystreptamine. Chem. Commun. 2860-2861 
    12. Ishikawa, J. and K. Hotta. 1999. FramePlot: A new implementation of the Frame analysis for predicting proteincoding regions in bacterial DNA with a high G+C content. FEMS Microhiol. Lett. 174: 251- 253 
    13. Kharel, M. K., S. Bimala, H. C. Lee, K. K. Liou, J. S. Woo, D. H. Kim, and J. K Sohng. 2003. Identification of 2Deoxy-scyllo-inosose synthase in aminoglycoside producer Streptomyces. J. Microbiol. Biotechnol. 13: 828- 831 
    14. Mingeot-Leclercq, M. P., Y. Glupczynski, and P. M. Tulkens, 1997. Aminoglycosides: Activity and resistance. Antimicrob. Agents Chemother. 43: 727- 737 
    15. Hyun, C. G., J. W. Kim, J. J. Han, Y. N. Choi, and J. W. Suh. 1998. Cloning and sequence analysis of the aminoglycoside resistance gene from a nebramycin complex producer, Streptoalloteichus hindustanus. J. Microbiol. Biotechnol. 8: 146-151 
    16. Kharel, M. K., D. B. Basnet, H. C. Lee, K. K. Liou, J. S. Woo, B. G. Kim, and J. K. Sohng. 2004. Isolation and characterization of the tobramycin biosynthetic gene cluster from Streptomyces tenebrarius. FEMS Microbiol. Lett. 230: 185- 190 
    17. Jo, Y. Y., S. H. Kim, Y. Y. Yang, C. M. Kang, J. K. Sohng, and J. W. Suh. 2003. Functional analysis of spectinomycin biosynthetic genes from Streptomyces spectabilis ATCC 27741. J. Microbiol, Biotechnol. 13: 906- 912 
    18. Carpet, F. 1988. Multiple sequence alignment with hierarchical clustering. Nucl. Acids Res. 16: 10881-10890 
    19. Kondo, S. and K. Hotta. 1998. Semisynthetic aminoglycoside antibiotics: Development and enzymatic modification. J. Infect. Chemother. 5: 1- 9 
    20. Lee, H. C., J. Y. Sohge, H. J. Kim, D. Y. Nam, C. N. Seong, J. M. Han, and J. C. Yoo. 2004. Cloning, expression, and biochemical characterization of dTDP-glucose 4,6-dehydratase gene (gerE) from Streptomyces sp. GERI-155. J. Microbiol. Biotechnol. 14: 576- 583 
    21. Ota, Y., H. Tamegai, F. Kudo, H. Kuriki, A. Koike-Takesida, T. Eguchi, and K. Kakinuma. 2000. Butirosin-biosynthetic gene cluster from Bacillus circulans. J. Antibiot. 53: 1158-1167 
    22. Sambrook, J. and D. W. Russell. 2001. Molecular Cloning, A Laboratory Manual. 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, U.S.A 
    23. Hashimoto, M., T. Kondo, I. Kozone, H. Kawaide, H. Abe, and M. Natsume. 2003. Relationship between respond to and production of aerial mycelium-inducing substances pamymycin 607 and A-factor. Biosci. Biotech. Biochem. 67: 803- 808 
    24. Joe, Y. A. and Y. M. Goo. 1998. Kanamycin acetyl transferase gene fom kanamycin-producing Streptomyces kanamyceticus IFO 13414. Arch. Pharm. Res. 21: 470- 474 
    25. Kwon, H. J., S. Y. Lee, S. K. Hong, U. M. Park, and J. W. Suh. 1999. Heterologous expression of Streptomyces albus genes linked to an integrating element and their roles in regulation of antibiotic production. J. Microbiol. Biotechnol. 9: 488- 497 
    26. Arakawa, K., R. Muller, T. Mahmud, T. W. Yu, and H. G. Floss. 2002. Characterization of the early stage aminoshikimate pathway in the formation of 3-amino-5-hydroxybenzoic acid: The RifN protein specifically converts kanosamine into kanosamine 6-phosphate. J. Am. Chem. Soc. 124: 10644-10645 
    27. Hyun, C. G., S. S. Kim, J. K. Sohng, J. J. Hahn, J. W. Kim, and J. W. Suh. 2000. An efficient approach for cloning the dNDP-glucose synthase gene from actinomycetes and its application in Streptomyces spectabills, a spectinomycin producer. FEMS Microbiol. Lett. 183: 183- 189 
    28. Nakano, M. M., H. Mashiko, and H. Ogawara. 1984. Cloning of the kanamycin resistance gene from a kanamycin producing Streptomyces species. J. Bacteriol. 157: 79- 83 
    29. Recht, M. I., S. Douthwaite, and J. D. Puglisi. 1999. Basis for prokaryotic specificity of action of aminoglycoside antibiotics. EMBO J. 18: 3133- 3138 
    30. Kieser, T., M. J. Bibb, M. J. Buttner, K. F. Chater, and D. A. Hopwood. 2000. Practical Streptomyces Genetics. The John Innes Foundation, Norwich, England 
    31. Piepersberg, W. 1997. Molecular biology, biochemistry and fermentation of aminoglycoside antibiotics, pp. 81- 163. In Strohl, W. R., Marcel-Dekker (eds.), Biotechnology of Antibiotics, Springer-Verlag Berlin Heidelberg, New York, U.S.A 
    32. Jung, Y. G., S. H. Kang, C. G. Hyun, Y. Y. Yang, C. M. Kang, and J. W. Suh. 2003. Isolation and characterization of bluensomycin biosynthetic genes from Streptomyces bluensis. FEMS Microbiol. Lett. 219: 285- 289 
  • 이 논문을 인용한 문헌 (9)

    1. 2006. "" Journal of microbiology and biotechnology, 16(1): 109~117     
    2. 2006. "" Journal of microbiology and biotechnology, 16(4): 590~596     
    3. 2006. "" Journal of microbiology and biotechnology, 16(6): 927~932     
    4. 2006. "" Journal of microbiology and biotechnology, 16(7): 1125~1131     
    5. 2006. "" Journal of microbiology and biotechnology, 16(8): 1311~1315     
    6. 2006. "" Journal of microbiology and biotechnology, 16(9): 1477~1480     
    7. 2007. "" Journal of microbiology and biotechnology, 17(10): 1708~1711     
    8. 2007. "" Journal of microbiology and biotechnology, 17(9): 1538~1545     
    9. 2008. "" Journal of microbiology and biotechnology, 18(5): 866~873     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기