본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.15 no.2, 2005년, pp.362 - 367   피인용횟수: 3

Screening and Characterization of Flocculent Yeast, Candida sp. HY200, for the Production of Xylitol from D-Xylose

KANG HEUI YUN    (Department of Molecular Science and Technology, College of Engineering, Ajou University   ); KIM YONG SUNG    (Department of Molecular Science and Technology, College of Engineering, Ajou University   ); KIM GEUN JOONG    (Institute of Biotechnological Industry, Inha University   ); SEO JIN HO    (Department of Agricultural Biotechnology, Seoul National University   ); RYU YEON WOO    (Department of Molecular Science and Technology, College of Engineering, Ajou University  );
  • 초록

    On the basis of high osmotic tolerance and xylitol production, a novel yeast strain was screened from soils of rice farming. The isolated strain HY200 was systematically characterized by using general approaches of Biolog Microlog $^{TM}$ and 18S rRNA sequence analyses, and consequently was designated as Candida tropicalis HY200. Under formulated culture conditions, relatively high xylitol yield ( $77\%$ ) and productivity (2.57 g/l $\codt$ h) were obtained, in practice, when 200 g/l of xylose was supplemented. In the utilization of nitrogen, inorganic compounds could not serve as nitrogen sources. As a promising phenotype, HY200 steadily flocculated during and/or after growing in the formulated medium. The extent of flocculation was partly affected by nitrogen sources. However, regardless of the kinds of carbon source fed, the flocculent cells were always observed at the end of the exponential growth phase. These observations strongly suggest that the strain HY200 could effectively be used as a potential candidate for the production of xylitol from xylose, especially in repeated batch mode, because of its flocculation ability and tolerance to high substrate concentrations.


  • 참고문헌 (32)

    1. Barbosa, M. F. S., M. B. de Medeiros, I. M. de Mancilha, H. Schneider, and H. Lee. 1988. Screening of yeasts for production of xylitol from D-xylose and some factors which affect xylitol yield in Candida guilliermondii. J. Ind. Microbiol. 3: 241-251 
    2. Dominguez, J. M., C. S. Gong, and G. T. Tsao. 1997. Production of xylitol from D-xylose by Debayomyces hansenii. Appl. Biochem. Biotechnol. 63: 117- 127 
    3. Emodi, A. 1978. Xylitol: Its properties and food applications. Food Techol. 32: 20- 32 
    4. Furlan, S. A., P. Bouilloud, and H. F. Castro. 1994. Influence of oxygen on ethanol and xylitol production by xylose fermenting yeasts. Process Biochem. 29: 657- 662 
    5. Furlan, S. A., P. Bouilloud, P. Strehaiano, and J. P. Riba. 1991. Study of xylitol formation under oxygen limited conditions. Biotechnol. Lett. 13: 203- 206 
    6. Horitsu, H., Y. Yahashi, K. Takamizawa, K. Kawai, T. Suzuki, and N. Watanabe. 1992. Production of xylitol from D-xylose by Candida tropicalis: Optimization of production rate. Biotechnol. Bioeng. 40: 1085-1091 
    7. Lee, W. J., M. D. Kim, M. S. Yoo, Y. W. Ryu, and J. H. Seo. 2003. Effects of xylose reductase activity on xylitol production in two-substrate fermentation of recombinant Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 13: 725- 730 
    8. Ligthelm, M. E., B. A. Prior, J. C du Preez, and V. Brandt. 1988. An investigation of $D-(1-^{13}C)$ xylose metabolism in Pichia stipitis under aerobic and anaerobic conditions. Appl. Microhiol. Biotechnol. 28: 293- 296 
    9. Roseiro, J. C., M. A. Peito, F. M. Girio, and M. AmaralCallaco. 1991. The effects of oxygen transfer coefficient and substrate concentration on xylose fermentation by Debarvomyces hansenii. Arch. Microbiol. 156: 484- 490 
    10. Roca, E., N. Meinander, and B. Hahn-Hagerdal, 1996. Xylitol production by immobilized recombinant Saccharomyces cerevisiae in a continuous packed-bead reactor. Biotechnol. Bioeng. 51: 317-326 
    11. Lee, J. H., Y. B. Lim, J. H. Koh, S. Y. Baig, and H. T. Shin. 2002. Screening of thermotolerant yeast for use as Microbiol feed additive. J. Microbiol. Biotechnol. 12: 162-165 
    12. Maekinen, K. K. 1979. Xylitol and oral health. Adv. Food Res. 25: 137- 158 
    13. Washuttle, J., P. Riederer, and E. Banchen. 1973. A qualitative and quantitative study of sugar-alcohols in several foods. J. Food Sci. 38: 1262 
    14. Ylikahri, R. 1979. Metabolic and nutritional aspects of xylitol. Adv. Food Res. 25: 159- 180 
    15. Du Preez, J. C., M. Bosch, and B. A. Prior. 1986. Xylose fermentation by Candida shehatae and Pichia stipitis: Effects of pH, temperature and substrate concentration. Enzyme Microb. Technol. 8: 360- 364 
    16. Ligthelm, M. E., B. A. Prior, and J. C. du Preez. 1988. The oxygen requirements of yeasts for the fermentation of D-xylose and D-gucose to ethanol. Appl. Microbiol. Biotechnol. 28: 63- 68 
    17. Walther. T., P. Hensirisak, and F. A. Agblevor. 2001. The influence of aeration and hemicellulosic sugars on xylitol production by Candida tropicalis. Biores. Technol. 76: 213-220 
    18. Gong, C. S., L. F. Chen, and G. T. Taso. 1981. Quantitative production of xylitol from D-xylose by high xylitol producing yeast mutant Candida tropicalis HXP2. Biotech. Lett. 3: 130-135 
    19. Sirisansanneeyakul, S., M. Staniszewski, and M. Rizzi. 1995. Screening of yeasts for production of xylitol from D-xylose. J. Ferment. Bioeng. 80: 565- 570 
    20. Vandeska, E., S. Amartey, S. Kuzmanova, and T. W. Jeffries. 1995. Effects of environmental conditions on production of xylitol by Candida boidinii. World J. Microbiol. Biotechnol. 11: 213- 218 
    21. Yahashi, Y., M. Hatsu, H. Horitsu, K. Kawai, T. Suzuki, and K. Takamizawa. 1996. D-Glucose feeding for improvement of xylitol productivity from D-xylose using Candida tropicalis immobilized on a non-woven fabric. Biotechnol. Lett. 18: 1395- 1400 
    22. Kim, S. Y., J. H. Kim, and D. K. Oh. 1997. Improvement of xylitol production by controlling oxygen supply in Candida parapsilosis. J. Ferment. Bioeng. 83: 267- 270 
    23. Kitpreechsvanich, V., M. Hayashi, N. Nishio, and S. Nagai. 1984. Conversion of D-xylose into xylitol by xylose reductase from Candida pelliculosa coupled with oxidoreductase system of methanogen strain HU. Biotechnol. Lett. 6: 651- 656 
    24. Lu, Jean, L. B. Tsai, C. S. Gong, and G. T. Tsao. 1995. Effect of nitrogen sources on xylitol production from D-xylose by Candida sp. L-102. Biotechnol. Lett. 17: 167-170 
    25. Nolleau, V., L. Preziosi-Belloy, J. P. Delgenes, and J. M. Navarro. 1993. Xylitol production from xylose by two yeast strains: Sugar tolerance. Curr. Microbiol. 27: 191- 197 
    26. Jeffries, T. W. 1981. Conversion of xylose to ethanol under aerobic conditions by Candida tropicalis. Biotechnol. Bioeng. 24: 371- 384 
    27. Hyvoenen, L., P. Koivistoinen, and F. Voirol. 1983. Food technological evaluation of xylitol. Adv. Food Res. 28: 373-403 
    28. Ikeuchi, T., M. Azuma, J. Kato, and H. Ooshirna. 1999. Screening of microorganisms for xylitol production and fermentation behavior in high concentrations of xylose. Biomass Bioenerg. 16: 333- 339 
    29. Jang, S. H., H. Y. Kang, G. J. Kim, J. H. Seo, and Y. W. Ryu. 2003. Complete in vitro conversion of D-xylose to xylitol by coupling xylose reductase and formate dehydrogenase. J. Microbiol. Biotechnol. 13: 501- 508 
    30. Kim, M. S., Y. S. Chung, J. H. Seo, D. H. Jo, Y. H. Park, and Y. W. Ryu. 2001. High-yield production of xylitol from xylose by a xylitol dehydrogenase defective mutant of Pichia stipitis. J. Microbiol, Biotechnol. 11: 564- 569 
    31. Oh, D. K. and S. Y. Kim. 1998. Increase of xylitol yield by feeding xylose and glucose in Candida tropicalis. Appl. Microbiol. Biotechnol. 50: 419- 425 
    32. Vongsuvalert, V. and Y. Tani. 1989. Xylitol production by a methanol yeast Candida boidinii (Kloekera sp.) No. 2201. J. Ferment. Bioeng. 67: 35- 39 
  • 이 논문을 인용한 문헌 (3)

    1. 2006. "" Journal of microbiology and biotechnology, 16(12): 1874~1881     
    2. 2006. "" Journal of microbiology and biotechnology, 16(12): 1904~1911     
    3. 2007. "" Journal of microbiology and biotechnology, 17(2): 297~304     

 저자의 다른 논문

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기