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Journal of microbiology and biotechnology v.15 no.2, 2005년, pp.368 - 375   피인용횟수: 7

Exo-Polysaccharide Production in Liquid Culture of Pleurotus ferulae

CHOI DU BOK    (Department of Chemical Engineering, Chosun University   ); KANG SI HYUNG    (Department of Chemical Engineering, Chosun University   ); SONG YON HO    (Department of Chemical Engineering, Chosun University   ); KWUN KYU HYUK    (Department of Chemical Engineering, Chosun University   ); JEONG KYOUNG JU    (Jeollanamdo Agricultural Research & Extension Services   ); CHA WOL SUK    (Department of Chemical Engineering, Chosun University  );
  • 초록

    Batch cultures were carried out to optimize the exo-polysaccharide production by liquid cultures of Pleurotus ferulae. Among the various carbon sources, when $5\%$ of glucose was used, the maximum mycelial growth and exo-polysaccharide concentration reached were 8.78 g/l and 3.59 g/l, respectively. Yeast extract and polypeptone were identified as the most suitable nitrogen sources. In particular, when a mixture of $1\%$ of polypeptone and $0.8\%$ of yeast extract was used, 9.52 g/l of mycelial growth and 4.09 g/l of exo-polysaccharide were obtained. In the case of mineral sources, K $_2$ HPO $_4$ and MgSO $_4$ $\codt$ 7H $_2$ 0 were found to be the best mineral sources for mycelial growth and exo-polysaccharide production. Under the optimized culture conditions, the agitation speed and aeration were investigated for mycelial growth and exo­polysaccharide production in a jar fermentor. The maximum mycelial growth and exo-polysaccharide concentration at 1.5 vvm and 200 rpm obtained were 13.2 g/l and 4.95 g/l, respectively, after 10 days of culture, which were $76\%$ and $79\%$ higher than those of the basal medium. The specific growth rate was decreased with the increase of mycelial growth. However, the specific production rate of the exo-polysaccharide was proportionally increased with the specific growth rate. The proposed model profiles showed good agreement with the experimental results for the mycelial growth and exo-polysaccharide production. The specific production rate using the optimized medium was higher than that of basal medium.


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