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Journal of microbiology and biotechnology v.15 no.2, 2005년, pp.447 - 450   피인용횟수: 5

Increased Production of Exoinulinase in Saccharomyces cerevisiae by Expressing the Kluyveromyces marxianus INU1 Gene Under the Control of the INU1 Promoter

KIM HEE EUN    (Division of Biological Sciences, and Research Center for Industrial Development of Biofood Materials   ); QIN RUI    (Basic Science Research Institute, Chonbuk National University   ); CHAE KEON SANG    (Division of Biological Sciences, and Research Center for Industrial Development of Biofood Materials  );
  • 초록

    Aspergillus niger inuE gene and Kluyveromyces marxianus INUI gene coding for exoinulinase were expressed in Saccharomyces cerevisiae under the control of K. marxianus INUI promoter. Recombinant S. cerevisiae expressing K. marxianus exoinulinase produced maximum 85 U/ml into culture medium, which was 9- to 14-fold higher than the activity produced by any other strain reported so far. In addition, K. marxianus INUI promoter produced 20- fold higher activity than S. cerevisiae glyceraldehydes phosphate dehydrogenase (GPD) promoter in S. cerevisiae.


  • 참고문헌 (12)

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  • 이 논문을 인용한 문헌 (5)

    1. 2006. "" Journal of microbiology and biotechnology, 16(2): 308~311     
    2. 2006. "" Journal of microbiology and biotechnology, 16(6): 979~982     
    3. 2006. "" Journal of microbiology and biotechnology, 16(9): 1459~1463     
    4. 2007. "" Journal of microbiology and biotechnology, 17(1): 37~43     
    5. 2007. "" Journal of microbiology and biotechnology, 17(3): 530~533     

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