본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Macromolecular research v.13 no.3, 2005년, pp.257 - 264  

Immobilization of Lactobionic Acid on Polyurethane Films and Their Interaction with Hepatocytes

Meng Wan    (Department of Chemical Engineering and Polymer Science, Yanbian University, Department of Polymer Science, Kyungpook National University   ); Jung Kyung-Hye    (Department of Polymer Science, Kyungpook National University   ); Kang Inn-Kyu    (Department of Polymer Science, Kyungpook National University   ); Kwon Oh Hyeong    (Department of Polymer Science & Engineering, Kumoh National Institute of Technology   ); Akaike Toshihiro    (Department of Biomolecular Engineering, Tokyo Institute of Technology  );
  • 초록

    Polyurethanes containing z-Iysine segments in the main chain (PULL) were synthesized from 4,4'-diphe-nylmethyl diisocyanate, poly(tetramethylene glycol), and z-Iysine oligomer as a chain extender. The PULL film was treated first with a $10\%$ HBr-acetic acid solution and subsequently with a saturated sodium bicarbonate aqueous solution to produce a primary amine group on the surface (PULL-N). Lactobionic acid (LA)-immobilized PULL (PULL-L) was prepared by the coupling reaction of the PULL surface amine groups and the LA carboxylic acid groups. The surface-modified PULLs were then characterized by attenuated total reflection-Fourier transform infra-red spectroscopy, electron spectroscopy for chemical analysis, atomic force microscopy, and contact angle goniometry. In the hepatocytes adhesion experiment, the cells poorly adhered to the PULL surface, although they adhered moderately well to the PULL-N surface. On the other hand, the cells adhered well to the PULL-L surface, suggesting the good affinity of the surface $\beta$ -galactose moieties for hepatocytes. When hepatocytes were cultured in the presence of epidermal growth factor for 48 h, the cells rapidly aggregated on the PULL-L surface, whereas they aggregated only slowly on the other surfaces. The PULL prepared in this study has the potential to be used as a coating material for the enhancement of hepatocyte adhesion.


  • 주제어

    films .   polyurethanes .   surfaces .   hepatocytes.  

  • 참고문헌 (39)

    1. S. Duest, J. Leslie, R. Moore, and K. Amplatz, Radiology, 113, 599 (1974) 
    2. V. Chytry, D. Letoumeur, M. Baudys, and J. Jozefonvicz, J. Biomed. Mater. Res., 31, 265 (1996) 
    3. I.-K. Kang, O. H. Kwon, Y. M. Lee, and Y. K. Sung, Biomaterials, 17, 841 (1996) 
    4. J. A. Rowley, G. Madlabayan, and D. J. Mooney, Biomaterials, 20, 45 (1999) 
    5. Y. C. Lee and R. T. Lee, Neoglycocorlfugates: Preparation and Application, Academic Press, San Diego, CA, 1994 
    6. R. Shibuta, M. Tanaka, M. Sisido, and Y. Imanishi, J. Biomed. Mater. Res., 20, 971 (1986) 
    7. D. K. Clark and A. Dilks, J. Polym. Sci.: Polym. Chem. Edn., 17, 957 (1979) 
    8. J. J. Yoon, Y. S. Nam, J. H. Kim, and T. G. Park, Biotech. Bioeng., 78, 1 (2002) 
    9. I.-K. Park, J. Yang, H. J. Jeong, H. S. Bom, I. Harada, T. Akaike, S. I. Kim, and C. S. Cho, Biomaterials, 24, 2331 (2003) 
    10. H. Ijima, K. Nakazawa, S. Koyama, M. Kaneko, T. Matsushita, T. Gion, K. Shirabe, M. Shimada, K. Takenaka, K. Sugimachi, and K. Funatsu, Int. J Artif. Organs., 23, 389 (2000) 
    11. G. Catapano, L. D. Bartolo, V. Vico, and L. Ambrosio, Biomaterials, 22, 659 (2001) 
    12. W. Meng, J. Y. Hyun, D. I. Song, and I.-K. Kang, J. Appl. Polym. Sci., 90, 1959 (2003) 
    13. W. Meng, J. Y. Hyun, D. I. Song, and I.-K. Kang, J. Appl. Polym. Sci., 90, 1959 (2003) 
    14. S. Johansson and M. Hook, J. Cell. Biol., 98, 810 (1984) 
    15. I.-K. Kang, G. J. Kim, O. H. Kwon, and Y. Ito, Biomaterials, 25, 4225 (2004) 
    16. X. L. Guo, K. S. Yang, J. H. Hyun, W. S. Kim, D. H. Lee, K. E. Min, L. S. Park, K. H. Seo, C. S. Cho, and I.-K. Kang, J. Biomater. Sci.: Polym. Edn., 14, 551 (2003) 
    17. J. Y. Hyun, W. Meng, I.-K. Kang, and D. I. Song, Polym. Adv. Technol., 14, 195 (2003) 
    18. I. Yang, M. Goto, H. Ise, C. S. Cho, and T. Akaike, Biomaterials, 23, 471 (2002) 
    19. Y. Ito, M. Sisido, and Y. Imanishi, J. Biomed. Mater. Res., 20, 1157 (1986) 
    20. I.-K. Kang, D. K. Baek, Y. M. Lee, and Y. K. Sung, J. Polym. Sci.: Polym. Chem., 36, 2331 (1998) 
    21. D. K. Han, K. D. Park, K. D. Ahn, S. Y. Jeong, and Y. H. Kim, J. Biomed. Mater. Res., 23, 87 (1989) 
    22. K. Kobayashi, H. Sumitomo, and Y. Ina, Polym. J., 15, 667 (1983) 
    23. A. Kobayashi, T. Akaike, K. Kobayashi, and H. Sumitomo, Macromol. Chem. Rapid. Commun., 7, 645 (1987) 
    24. S. A. Pahemik, W. E. Thasler, M. Doser, M. J. Gomez-Lechon, M. J. Castell, H. Planck, and H. G. Koebe, Cells. Tissues. Organs., 168, 170 (2001) 
    25. M. D. Lelah and S. L. Cooper, Polyurethane in Medicine, CRC Press, Boca Raton, FL,1986 
    26. K. D. Park, T. Okano, C. Nojiri, and S. W. Kim, J. Biomed. Mater. Res., 22, 977(1988) 
    27. S. T. Lopina, G. Wu, E. W. Merrill, and L. G. Cima, Biomaterials, 17, 559 (1996) 
    28. S.-H. Kim, M. Goto, and T. Akaike, J. Biol. Chem., 276, 35312 (2001) 
    29. A. Kobayashi, M. Goto, T. Sekine, A. Masumoto, N. Yamamoto, K. Kobayashi, and T. Akaike, Artif. Organs., 16, 564 (1992) 
    30. J. H. Lawson, D. B. Olsen, E. Hershgold, J. Kolff, K. Hadfield, and W. J. Kolff, Trans. Am. Artif Intern. Organs., 21, 368 (1975) 
    31. C. B. Wisman, W. S. Pierce, J. H. Donachy, W. E. Pae, J. L. Meyer, and G. A. Prophet, Trans. Am, Soc. Artif Intern. Organs., 28, 164 (1982) 
    32. J. H. Lee, J. W. Park, and H. B. Lee, Biomaterials, 12, 443 (1991) 
    33. Y. Sato, T. Ochiya, Y. Yasuda, and K. Matsubara, Hepatology, 19, 1023 (1994) 
    34. K. S. Yang, X. L. Guo, W. Meng, J. Y. Hyun, and L-K. Kang, Macromol. Res., 11, 488 (2003) 
    35. A. S. Yap, B. R. Stevenson, J. R. Keast, and S. W. Manley, Endocrinology, 136, 4672 (1995) 
    36. I.-K. Kang, D. W. Lee, S. K. Lee, and T. Akaike, J. Mater. Sci.: Mater. Med., 14, 611 (2003) 
    37. D.J. Mooney, L. K. Hansen, J. P. Vacanti, R. Langer, S. R. Farmer, and D. E. Ingber, J. Cell. Phys., 151, 497 (1992) 
    38. H. Kurosawa, K. Yasumoto, T. Kimura, and Y. Amano, Biotech. Bioeng., 70, 160 (2000) 
    39. B. M. Gumbiner, Cell, 84, 345 (1996) 

 저자의 다른 논문

  • Kang, Inn-Kyu (29)

    1. 1989 "생체적합성과 생분해성을 갖는 Polypeptide Copolymer의 합성과 물성에 관한 연구(II)" 의공학회지 = Journal of biomedical engineering research 10 (3): 237~242    
    2. 1990 "생체적합성과 생분해성을 갖는 Polypeptide Copolymer의 합성과 물성에 관한 연구. (1) 폴리에틸렌 글리콜을 그라프트시킨 폴리펩티드의 합성" 대한화학회지 = Journal of the Korean Chemical Society 34 (2): 197~202    
    3. 1992 "Poly($\tau$-benzy1 L-glutamate)와 polyoxpropylene으로 된 블록 공중합체의 합성 및 약물방출특성" 의공학회지 = Journal of biomedical engineering research 13 (1): 55~64    
    4. 1992 "펩티드성분을 함유하는 블록공중합체막의 세포접착성" 의공학회지 = Journal of biomedical engineering research 13 (2): 155~164    
    5. 1992 "새로운 생체적합성 폴리펩티드공중합체의 합성과 혈액적합성에 관한 연구 : 측쇄에 에틸렌글리콜을 함유하는 폴리펩티드 공중합체의 혈액적합성" 대한화학회지 = Journal of the Korean Chemical Society 36 (6): 947~954    
    6. 1993 "Poly(Amino Acid) Matrix를 이용한 스테로이드의 방출기구에 관한 연구" 대한화학회지 = Journal of the Korean Chemical Society 37 (4): 378~389    
    7. 1993 "생체적합성과 생분해성을 갖는 키틴유도체의 합성 및 물성 III -에스테르 키틴 유도체의 합성과 용해 특성-" 공업화학 = Applied chemistry for engineering 4 (4): 785~790    
    8. 1998 "Corona방전처리에 의한 PVC film의 대전방지가공" 韓國染色加工學會誌 = Textile coloration and finishing 10 (3): 43~49    
    9. 1999 "방전처리에 의한 Polypropylene섬유의 표면개질" 韓國染色加工學會誌 = Textile coloration and finishing 11 (2): 27~37    
    10. 2000 "일본의 고분자교육" 고분자 과학과 기술 = Polymer science and technology 11 (6): 766~776    

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기