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The journal of microbiology v.43 no.3, 2005년, pp.219 - 227   피인용횟수: 10

Isolation and Characterization of Bacteria Associated with Two Sand Dune Plant Species, Calystegia soldanella and Elymus mollis

Park Myung Soo    (Korea Research Institute of Bioscience and Biotechnology   ); Jung Se Ra    (Korea Research Institute of Bioscience and Biotechnology   ); Lee Myoung Sook    (Korea Research Institute of Bioscience and Biotechnology   ); Kim Kyoung Ok    (Korea Research Institute of Bioscience and Biotechnology   ); Do Jin Ok    (Korea Research Institute of Bioscience and Biotechnology   ); Lee Kang Hyun    (Korea Research Institute of Bioscience and Biotechnology   ); Kim Seung Bum    (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University   ); Bae Kyung Sook    (Korea Research Institute of Bioscience and Biotechnology  );
  • 초록

    Little is known about the bacterial communities associated with the plants inhabiting sand dune ecosystems. In this study, the bacterial populations associated with two major sand dune plant species, Calystegia soldanella (beach morning glory) and Elymus mollis (wild rye), growing along the costal areas in Tae-An, Chungnam Province, were analyzed using a culture-dependent approach. A total of 212 bacteria were isolated from the root and rhizosphere samples of the two plants, and subjected to further analysis. Based on the analysis of the 16S rDNA sequences, all the bacterial isolates were classified into six major phyla of the domain Bacteria. Significant differences were observed between the two plant species, and also between the rhizospheric and root endophytic communities. The isolates from the rhizosphere of the two plant species were assigned to 27 different established genera, and the root endophytic bacteria were assigned to 21. Members of the phylum Gammaproteobacteria, notably the Pseudomonas species, comprised the majority of both the rhizospheric and endophytic bacteria, followed by members of Bacteroidetes and Firmicutes in the rhizosphere and Alphaproteobacteria and Bacteroidetes in the root. A number of isolates were recognized as potentially novel bacterial taxa. Fifteen out of 27 bacterial genera were commonly found in the rhizosphere of both plants, which was comparable to 3 out of 21 common genera in the root, implying the host specificity for endophytic populations. This study of the diversity of culturable rhizospheric and endophytic bacteria has provided the basis for further investigation aimed at the selection of microbes for the facilitation of plant growth.


  • 주제어

    rhizobacteria .   endophytic bacteria .   sand dune plant .   16S rDNA sequence .   Pseudomonas.  

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  • 이 논문을 인용한 문헌 (10)

    1. 2006. "" The journal of microbiology, 44(6): 600~606     
    2. Do, Jin-Ok ; Park, Seong-Joo ; Kim, Seung-Bum 2007. "Molecular Systematic Study of Bacterial Community Associated with Sand Dune Plants" 환경생물 = Korean journal of environmental biology, 25(4): 356~362     
    3. 2007. "" Journal of microbiology and biotechnology, 17(8): 1361~1368     
    4. 2008. "" The journal of microbiology, 46(1): 12~15     
    5. Jung, Se-Ra ; Kim, Seung-Bum 2008. "Analysis of Community Level Physiological Profiles in the Rhizosphere of Brassica rapa subsp. pekinensis" 환경생물 = Korean journal of environmental biology, 26(1): 42~46     
    6. Lee, Jae-Eun ; Lee, Seo-Hyeun ; Park, Kyung-Soo ; Park, Jin-Woo ; Park, Kyung-Seok 2009. "Effect of Bacillus subtilis C4 and B. cereus D8 on Plant Growth of Canola and Controlling Activity Against Soft Rot and Stem Rot" 농약과학회지 = The Korean journal of pesticide science, 13(4): 275~282     
    7. 2009. "" The journal of microbiology, 47(1): 33~39     
    8. 2009. "" Journal of microbiology and biotechnology, 19(1): 99~107     
    9. 2010. "" The journal of microbiology, 48(5): 559~565     
    10. Shin, Young-Min ; Kim, Tae-Ui ; Choi, Ah-Young ; Chun, Jee-Sun ; Lee, Sang-Hoon ; Kim, Ha-Neul ; Yi, Ha-Na ; Jo, Jae-Hyung ; Cho, Jang-Cheon ; Jahng, Kwang-Yeop ; Kim, Kyu-Joong ; Joh, Ki-Seong ; Chun, Jong-Sik ; Lee, Hyune-Hwan ; Kim, Seung-Bum 2011. "Species Diversity of Betaproteobacteria in the Sumunmulbengdui Wetland Area of Jeju Island and Distribution of Novel Taxa" 환경생물 = Korean journal of environmental biology, 29(3): 154~161     

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