Evaluation of Negative Results of BacT/Alert 3D Automated Blood Culture System
Although automated continuous-monitoring blood culture systems are both rapid and sensitive, false-positive and false-negative results still occur. The objective of this study, then, was to evaluate negative results occurring with BacT/Alert 3D blood culture systems. A total of 1032 samples were cultured with the BacT/Alert 3D automated blood culture system, using both aerobic (BPA) and anaerobic (BPN) media, and 128 of these samples yielded positive results. A total of 904 negative blood samples were then subcultured in $5\%$ sheep blood agar, eosin methylene blue, chocolate agar, and sabouraud-dextrose agar. Organisms growing on these subcultures were subsequently identified using both Vitek32 (bioMerieux, Durham, NC) and conventional methods. Twenty four $(2.6\%)$ of the 904 subcultures grew on the subculture media. The majority $(83.3\%)$ of these were determined to be gram-positive microorganisms. Fourteen $(58.3\%)$ were coagulase-negative staphylococci, two $(8.3\%)$ were Bacillus spp., one $(4.2\%)$ was Staphylococcus aureus, and one $(4.2\%)$ was identified as Enterococcus faecium. Streptococcus pneumoniae and Neisseria spp. were isolated together in two $(8.3\%)$ vials. Gram-negative microorganisms comprised $12.5\%$ of the subcultures, of which two $(8.3\%)$ were found to be Pseudomonas aeruginosa, and one $(4.2\%)$ was Pseudomonas fluorescens. The other isolate $(4.2\%)$ was identified as Candida albicans. We conclude that the subculture of negative results is valuable in the BacT/Alert 3D system, especially in situations in which only one set of blood cultures is taken.
- Araj, G.F., R.L. Hopfer, M. Wenglar, and V. Fainstein. 1981. Valuable of terminal subcultures from negative BACTEC blood culture bottles. J. Clin. Microbiol. 14, 589-590
- Campbell, J. and J.A. Washington, 2nd. 1980. Evaluation of the necessity for routine terminal subcultures of previously negative blood cultures. J. Clin. Microbiol. 12. 576-578
- Horvath, L.L., B.J. George, C.K., Murray, L.S. Harrison, and D.R. Hospenthal. 2004. Direct comparison of the BACTEC 9240 and BacT/Alert3D automated blood culture systems for candida growth detection. J. Clin. Microbiol. 42, 115-118
- Shigei, J.T., J.A. Shimabukuro, M.T. Pezzlo, L.M. de la Maza, and E.M. Peterson. 1995. Value of terminal subcultures for blood cultures monitored by BACTEC 9240. J. Clin. Microbiol. 33, 1385-1388
- Seegmuller, I., U. Eschenbach, K. Kamereck, and T. Miethke. 2004. Sensitivity of the BacT/Alert FA-medium for detection of Pseudomonas aeruginosa in pre-incubated blood cultures and its temperature-dependence. J. Med. Microbiol. 53, 869-874
- Hardy, D.J., B.B. Hulbert, and P.C. Migneault. 1992. Time to detection of positive BacT/Alert blood cultures and lack of need for routine subculture of 5- to 7-day negative cultures. J. Clin. Microbiol. 30, 2743-2745
- Rohner, P., B. Pepey, and R. Auckenthaler. 1995. Comparison of BacT/Alert with Signal blood culture system. J. Clin. Microbiol. 33, 313-317
- Fontanals, D., I. Sanfeliu, I. Pons, D. Mariscal, and M. Torra. 1998. Evaluation of the BacT/Alert and VITAL blood culture systems for the diagnosis of bacteremia. Clin. Microbiol. Infect. 4, 88-93
- Qian, Q., Y.W. Tang, C.P. Kolbert, C.A. Torgerson, J.G. Hughes, E.A. Vetter, W.S. Harmsen, S.O. Montgomery, F.R. Cockerill, 3rd. and D.H. Persing. 2001. Direct identification of bacteria from positive blood cultures by amplification and sequencing of the 16S rRNA gene: evaluation of BACTEC 9240 instrument true-positive and false-positive results. J. Clin. Microbiol. 39, 3578-3582
- Schwabe, L.D., R.B. Jr. Thomson, K.K. Flint, and F.P. Koontz. 1995. Evaluation of BACTEC 9240 blood culture system by using high-volume aerobic resin media. J. Clin. Microbiol. 33, 2451-2453
- Gimenez, M., C. Prat, X. Valles, L. Matas, J. Arnal, and V. Ausina. 2002. Evaluation of the VITAL (bioMerieux) automated blood culture system using blind subculture. Clin. Microbiol. Infect. 8, 222-228
- Alfa, M., S. Sanche, S. Roman, Y. Fiola, P. Lenton, and G. Harding. 1995. Continuous quality improvement for introduction of automated blood culture instrument. J. Clin. Microbiol. 33, 1185-1191
- Mirrett, S., L.B. Reller, C.A. Petti, C.W. Woods, B. Vazirani, R. Sivadas, and M.P. Weinstein. 2003. Controlled clinical comparison of BacT/Alert standard aerobic medium with BACTEC standard aerobic medium for culturing blood. J. Clin. Microbiol. 41, 2391-2394
- Nicholls, T.M., A.S. Morgan, and A.J. Morris. 2000. Nosocomial blood stream infection in Auckland Healtcare hospitals. N. Z. Med. J. 113, 96-98
- Daxboeck, F., H.J. Dornbusch, R. Krause, O. Assadian, and C. Wenisch. 2004. Verification of false-positive blood culture results generated by the BACTEC 9000 series by eubacterial 16S rDNA and panfungal 18S rDNA directed polymerase chain reaction (PCR). Diagn. Microbiol. Infect. Dis. 48, 1-3
- Wellinghausen, N., B. Wirths, A.R. Franz, L. Karolyi, R. Marre, and U. Reischl. 2004. Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time Light- Cycler polymerase chain reaction (PCR) with sequencespecific probes. Diagn. Microbiol. Infect. Dis. 48, 229-241
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- Sung, Hye-Ran ; Kim, Il-Hoi ; Kim, Jee-Youn ; Lee, Chong-Kil ; Chung, Yeon-Bok ; Han, Sang-Bae ; Song, Suk-Gil 2008. "Detection of Microbial Growth in an Automated Culture System" Korean journal of microbiology = 미생물학회지, 44(2): 130~134
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