본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

The journal of microbiology v.43 no.3, 2005년, pp.257 - 259   피인용횟수: 1

Evaluation of Negative Results of BacT/Alert 3D Automated Blood Culture System

Kocoglu M. Esra    (Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Gaziantep University   ); Bayram Aysen    (Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Gaziantep University   ); Balcl Iclal    (Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Gaziantep University  );
  • 초록

    Although automated continuous-monitoring blood culture systems are both rapid and sensitive, false-positive and false-negative results still occur. The objective of this study, then, was to evaluate negative results occurring with BacT/Alert 3D blood culture systems. A total of 1032 samples were cultured with the BacT/Alert 3D automated blood culture system, using both aerobic (BPA) and anaerobic (BPN) media, and 128 of these samples yielded positive results. A total of 904 negative blood samples were then subcultured in $5\%$ sheep blood agar, eosin methylene blue, chocolate agar, and sabouraud-dextrose agar. Organisms growing on these subcultures were subsequently identified using both Vitek32 (bioMerieux, Durham, NC) and conventional methods. Twenty four $(2.6\%)$ of the 904 subcultures grew on the subculture media. The majority $(83.3\%)$ of these were determined to be gram-positive microorganisms. Fourteen $(58.3\%)$ were coagulase-negative staphylococci, two $(8.3\%)$ were Bacillus spp., one $(4.2\%)$ was Staphylococcus aureus, and one $(4.2\%)$ was identified as Enterococcus faecium. Streptococcus pneumoniae and Neisseria spp. were isolated together in two $(8.3\%)$ vials. Gram-negative microorganisms comprised $12.5\%$ of the subcultures, of which two $(8.3\%)$ were found to be Pseudomonas aeruginosa, and one $(4.2\%)$ was Pseudomonas fluorescens. The other isolate $(4.2\%)$ was identified as Candida albicans. We conclude that the subculture of negative results is valuable in the BacT/Alert 3D system, especially in situations in which only one set of blood cultures is taken.


  • 주제어

    BacT/Alert 3D .   false-negative results .   subculture.  

  • 참고문헌 (16)

    1. Araj, G.F., R.L. Hopfer, M. Wenglar, and V. Fainstein. 1981. Valuable of terminal subcultures from negative BACTEC blood culture bottles. J. Clin. Microbiol. 14, 589-590 
    2. Campbell, J. and J.A. Washington, 2nd. 1980. Evaluation of the necessity for routine terminal subcultures of previously negative blood cultures. J. Clin. Microbiol. 12. 576-578 
    3. Horvath, L.L., B.J. George, C.K., Murray, L.S. Harrison, and D.R. Hospenthal. 2004. Direct comparison of the BACTEC 9240 and BacT/Alert3D automated blood culture systems for candida growth detection. J. Clin. Microbiol. 42, 115-118 
    4. Shigei, J.T., J.A. Shimabukuro, M.T. Pezzlo, L.M. de la Maza, and E.M. Peterson. 1995. Value of terminal subcultures for blood cultures monitored by BACTEC 9240. J. Clin. Microbiol. 33, 1385-1388 
    5. Seegmuller, I., U. Eschenbach, K. Kamereck, and T. Miethke. 2004. Sensitivity of the BacT/Alert FA-medium for detection of Pseudomonas aeruginosa in pre-incubated blood cultures and its temperature-dependence. J. Med. Microbiol. 53, 869-874 
    6. Hardy, D.J., B.B. Hulbert, and P.C. Migneault. 1992. Time to detection of positive BacT/Alert blood cultures and lack of need for routine subculture of 5- to 7-day negative cultures. J. Clin. Microbiol. 30, 2743-2745 
    7. Rohner, P., B. Pepey, and R. Auckenthaler. 1995. Comparison of BacT/Alert with Signal blood culture system. J. Clin. Microbiol. 33, 313-317 
    8. Fontanals, D., I. Sanfeliu, I. Pons, D. Mariscal, and M. Torra. 1998. Evaluation of the BacT/Alert and VITAL blood culture systems for the diagnosis of bacteremia. Clin. Microbiol. Infect. 4, 88-93 
    9. Qian, Q., Y.W. Tang, C.P. Kolbert, C.A. Torgerson, J.G. Hughes, E.A. Vetter, W.S. Harmsen, S.O. Montgomery, F.R. Cockerill, 3rd. and D.H. Persing. 2001. Direct identification of bacteria from positive blood cultures by amplification and sequencing of the 16S rRNA gene: evaluation of BACTEC 9240 instrument true-positive and false-positive results. J. Clin. Microbiol. 39, 3578-3582 
    10. Schwabe, L.D., R.B. Jr. Thomson, K.K. Flint, and F.P. Koontz. 1995. Evaluation of BACTEC 9240 blood culture system by using high-volume aerobic resin media. J. Clin. Microbiol. 33, 2451-2453 
    11. Gimenez, M., C. Prat, X. Valles, L. Matas, J. Arnal, and V. Ausina. 2002. Evaluation of the VITAL (bioMerieux) automated blood culture system using blind subculture. Clin. Microbiol. Infect. 8, 222-228 
    12. Alfa, M., S. Sanche, S. Roman, Y. Fiola, P. Lenton, and G. Harding. 1995. Continuous quality improvement for introduction of automated blood culture instrument. J. Clin. Microbiol. 33, 1185-1191 
    13. Mirrett, S., L.B. Reller, C.A. Petti, C.W. Woods, B. Vazirani, R. Sivadas, and M.P. Weinstein. 2003. Controlled clinical comparison of BacT/Alert standard aerobic medium with BACTEC standard aerobic medium for culturing blood. J. Clin. Microbiol. 41, 2391-2394 
    14. Nicholls, T.M., A.S. Morgan, and A.J. Morris. 2000. Nosocomial blood stream infection in Auckland Healtcare hospitals. N. Z. Med. J. 113, 96-98 
    15. Daxboeck, F., H.J. Dornbusch, R. Krause, O. Assadian, and C. Wenisch. 2004. Verification of false-positive blood culture results generated by the BACTEC 9000 series by eubacterial 16S rDNA and panfungal 18S rDNA directed polymerase chain reaction (PCR). Diagn. Microbiol. Infect. Dis. 48, 1-3 
    16. Wellinghausen, N., B. Wirths, A.R. Franz, L. Karolyi, R. Marre, and U. Reischl. 2004. Algorithm for the identification of bacterial pathogens in positive blood cultures by real-time Light- Cycler polymerase chain reaction (PCR) with sequencespecific probes. Diagn. Microbiol. Infect. Dis. 48, 229-241 
  • 이 논문을 인용한 문헌 (1)

    1. Sung, Hye-Ran ; Kim, Il-Hoi ; Kim, Jee-Youn ; Lee, Chong-Kil ; Chung, Yeon-Bok ; Han, Sang-Bae ; Song, Suk-Gil 2008. "Detection of Microbial Growth in an Automated Culture System" Korean journal of microbiology = 미생물학회지, 44(2): 130~134     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기