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Journal of food science and nutrition v.10 no.2, 2005년, pp.134 - 139   피인용횟수: 16

Screening for Angiotensin 1-Converting Enzyme Inhibitory Activity of Ecklonia cava

Athukorala Yasantha    (Faculty of Applied Marine Science, Cheju National University   ); Jeon, You-Jin    (Faculty of Applied Marine Science, Cheju National University  );
  • 초록

    Seven brown algal species (Ecklonia cava, Ishige okamurae, Sargassum fulvellum, Sargassum horneri, Sargassum coreanum, Sargassum thunbergii and Scytosiphon lomentaria) were hydrolyzed using five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase) and screened for angiotensin 1-converting enzyme (ACE) inhibitory activities. Most algal species examined showed good ACE inhibitory activities after the enzymatic hydrolysis. However, E. cava was the most potent ACE inhibitor of the seven species. Flavourzyme digest of E. cava exhibited an $IC_{50}$ of around $0.3\;{\mu}g/mL$ for ACE; captopril has an $IC_{50}$ of $\~0.05\;{\mu}g/mL$ . The Flavourzyme digest was separated to three fractions by an ultrafiltration membrane (5, 10, 30 kDa MWCO) system according to the molecular weights. The active components were mainly concentrated in >30 kD fraction which are composed of the highest protein content $(27\%)$ and phenolic content (261 mg/100 mL) compared to the other two smaller molecular weight fractions. Therefore, the active compounds appear to be relatively high molecular weight complex molecules associated with protein (glycoprotein) and polyphenols. Therefore, E. cave is a potential source of antihypertensive compound.


  • 주제어

    brown algae .   Ecklonia cava .   ACE inhibition .   enzymatic hydrolysis .   Flavourzyme.  

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    3. Cha, Seon-Heui ; Lee, Ki-Wan ; Jeon, You-Jin 2006. "" Algae, 21(3): 343~348     
    4. Cha, Seon-Heui ; Ahn, Gin-Nae ; Heo, Soo-Jin ; Kim, Kil-Nam ; Lee, Ki-Wan ; Song, Choon-Bok ; K.Cho, So-Mi ; Jeon, You-Jin 2006. "Screening of Extracts from Marine Green and Brown Algae in Jeju for Potential Marine Angiotensin-I Converting Enzyme (ACE) Inhibitory Activity" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 35(3): 307~314     
    5. 2006. "" Journal of microbiology and biotechnology, 16(5): 757~763     
    6. Kim, Y.J. ; Chin, Koo-Bok 2007. "Evaluation of Angiotensin -I- Converting Enzyme Inhibitory Activity and Protein Changes of Enzymatic Hydrolysate Extracted from Hanwoo Loin and Round Myosin B" 한국동물자원과학회지 = Journal of animal science and technology, 49(1): 129~136     
    7. Heu, Min-Soo ; Yoon, Min-Seok ; Kim, Hyung-Jun ; Park, Kwon-Hyun ; Lee, Jong-Hyun ; Jo, Mi-Ran ; Lee, Jung-Suk ; Jeon, You-Jin ; Kim, Jin-Soo 2010. "Improvement on the Antioxidant Activity of Instant Noodles Containing Enzymatic Extracts from Ecklonia cava and Its Quality Characterization" 한국수산과학회지 = Korean journal of fisheries and aquatic sciences, 43(5): 391~399     
    8. Cha, Seon-Heui ; Kim, Min-Joo ; Yang, Hye-Young ; Jin, Chang-Beum ; Jeon, You-Jin ; Oda, Tatsuya ; Kim, Dae-Kyung 2010. "ACE, α-Glucosidase and Cancer Cell Growth Inhibitory Activities of Extracts and Fractions from Marine Microalgae, Nannochloropsis oculata" 한국수산과학회지 = Korean journal of fisheries and aquatic sciences, 43(5): 437~444     
    9. Ko, Seok-Chun ; Kang, Sung-Myung ; Ahn, Gin-Nae ; Yang, Hyun-Pil ; Kim, Kil-Nam ; Jeon, You-Jin 2010. "Antioxidant Activity of Enzymatic Extracts from Sargassum coreanum" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 39(4): 494~499     
    10. 2010. "" Fisheries and aquatic sciences, 13(1): 26~35     
    11. 2011. "" Nutrition research and practice, 5(2): 93~100     
    12. Park, Kwon-Hyun ; Lee, Ji-Sun ; Shin, Joon-Ho ; Lee, Jong-Hyun ; Jo, Mi-Ran ; Jeon, You-Jin ; Kim, Jin-Soo 2011. "Processing Optimization of Ecklonia cava Extract-Added Seasoning Sauce for Instant Noodles" 한국수산과학회지 = Korean journal of fisheries and aquatic sciences, 44(3): 197~206     
    13. 2011. "" Algae, 26(2): 201~208     
    14. 2012. "" Algae, 27(3): 205~213     
    15. Jwa, Min-Seok ; Yeo, In-Kyu 2015. "Effects of dietary supplementation with citrus pomace and Ecklonia cava residue on the physiological changes and growth of disk abalone, Haliotis discus discus" 韓國魚病學會誌= Journal of fish pathology, 28(1): 53~62     
    16. Park, Ji-Hye ; Bae, Nan-Young ; Park, Sun-Hee ; Kim, Min-Ji ; Kim, Koth-Bong-Woo-Ri ; Choi, Jung-Su ; Ahn, Dong-Hyun 2015. "Antioxidant Effect of Sargassum coreanum Root and Stem Extracts" KSBB Journal, 30(4): 155~160     

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