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Higher Expression of TRPM7 Channels in Murine Mature B Lymphocytes than Immature Cells

Kim, Jin-Kyoung   (Department of Anesthesiology and Pain Medicine, Samsung Medical CenterHH0000056  ); Ko, Jae-Hong   (Department of Physiology and Biophysics, Seoul National University College of MedicineUU0000691  ); Nam, Joo-Hyun   (Department of Physiology, Sungkyunkwan University School of MedicineUU0000759  ); Woo, Ji-Eun   (Department of Physiology, Sungkyunkwan University School of MedicineUU0000759  ); Min, Kyeong-Min   (Department of Physiology, Sungkyunkwan University School of MedicineUU0000759  ); Earm, Yung-E   (Department of Physiology and Biophysics, Seoul National University College of MedicineUU0000691  ); Kim, Sung-Joon   (Department of Physiology and Biophysics, Seoul National University College of MedicineUU0000691  );
  • 초록

    TRPM7, a cation channel protein permeable to various metal ions such as $Mg^{2+}$ , is ubiquitously expressed in variety of cells including lymphocytes. The activity of TRPM7 is tightly regulated by intracellular $Mg^{2+}$ , thus named $Mg^{2+}$ -inhibited cation (MIC) current, and its expression is known to be critical for the viability and proliferation of B lymphocytes. In this study, the level of MIC current was compared between immature (WEHI-231) and mature (Bal-17) B lymphocytes. In both cell types, an intracellular dialysis with $Mg^{2+}$ -free solution (140 mM CsCl) induced an outwardly-rectifying MIC current. The peak amplitude of MIC current and the permeability to divalent cation ( $Mn^{2+}$ ) were several fold higher in Bal-17 than WEHI-231. Also, the level of mRNAs for TRPM7, a molecular correspondence of the MIC channel, was significantly higher in Bal-17 cells. The amplitude of MIC was further increased, and the relation between current and voltage became linear under divalent cation-free conditions, demonstrating typical properties of the TRPM7. The stimulation of B cell receptors (BCR) by ligation with antibodies did not change the amplitude of MIC current. Also, increase of extracellular $[Mg^{2+}]_c$ to enhance the $Mg^{2+}$ influx did not affect the BCR ligation-induced death of WEHI-231 cells. Although the level of TRPM7 was not directly related with the cell death of immature B cells, the remarkable difference of TRPM7 might indicate a fundamental change in the permeability to divalent cations during the development of B cells.


  • 주제어

    B lymphocyte .   TRPM7 .   Nonselective cation channel .   Cell death .   Bal-17 .   WEHI-231.  

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  • 이 논문을 인용한 문헌 (1)

    1. 2006. "" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 10(1): 19~24     

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