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Regulation of L-type Calcium Channel Current by Somatostatin in Guinea-Pig Gastric Myocytes

Kim, Young-Chul    (Department of Physiology, Chungbuk National University College of Medicine   ); Sim, Jae-Hoon    (Department of Physiology and Biophysics, Seoul National University College of Medicine   ); Lee, Sang-Jin    (Department of Physiology, Chungbuk National University College of Medicine   ); Kang, Tong-Mook    (Department of Physiology, Sungkyunkwan University School of Medicine   ); Kim, Sung-Joon    (Department of Physiology and Biophysics, Seoul National University College of Medicine   ); Kim, Seung-Ryul    (Department of Biochemistry, Chungbuk National University College of Medicine   ); Youn, Sei-Jin    (Department of Internal Medicine, Chungbuk National University College of Medicine   ); Lee, Sang-Jeon    (Department of Surgery, Chungbuk National University College of Medicine   ); Xu, Wen Xie    (Department of Physiology, Medical School, Shaghai Jiao Tong University   ); So, In-Suk    (Department of Physiology and Biophysics, Seoul National University College of Medicine   ); Kim, Ki-Whan    (Department of Physiology and Biophysics, Seoul National University College of Medicine  );
  • 초록

    To study the direct effect of somatostatin (SS) on calcium channel current ( $I_{Ba}$ ) in guinea-pig gastric myocytes, $I_{Ba}$ was recorded by using whole-cell patch clamp technique in single smooth muscle cells. Nicardipine ( $1{\mu}M$ ), a L-type $Ca^{2+}$ channel blocker, inhibited $I_{Ba}$ by $98{\pm}1.9$ % (n=5), however $I_{Ba}$ was decreased in a reversible manner by application of SS. The peak $I_{Ba}$ at 0 mV were decreased to $95{\pm}1.5$ , $92{\pm}1.9$ , $82{\pm}4.0$ , $66{\pm}5.8$ , $10{\pm}2.9$ % at $10^{-10}$ , $10^{-9}$ , $10^{-8}$ , $10^{-7}$ , $10^{-5}$ M of SS, respectively (n=3∼6; $mean{\pm}SEM$ ). The steady-state activation and inactivation curves of $I_{Ba}$ as a function of membrane potentials were well fitted by a Boltzmann equation. Voltage of half-activation ( $V_{0.5}$ ) was $-12{\pm}0.5$ mV in control and $-11{\pm}1.9$ mV in SS treated groups (respectively, n=5). The same values of half-inactivation were $-35{\pm}1.4$ mV and $-35{\pm}1.9$ mV (respectively, n=5). There was no significant difference in activation and inactivation kinetics of $I_{Ba}$ by SS. Inhibitory effect of SS on $I_{Ba}$ was significantly reduced by either dialysis of intracellular solution with $GDP_{\beta}S$ , a non-hydrolysable G protein inhibitor, or pretreatment with pertussis toxin (PTX). SS also decreased contraction of guinea-pig gastric antral smooth muscle. In conclusion, SS decreases voltage-dependent L-type calcium channel current ( $VDCC_L$ ) via PTXsensitive signaling pathways in guinea-pig antral circular myocytes.


  • 주제어

    Gastric myocytes .   Calcium current .   Somatostatin .   G-protein.  

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