본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Effects of Sphingosine-1-phosphate, Furosemide and Indomethacin on Mucin Release from Airway Goblet Cells

Kim, Ju-Ock    (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital   ); Kim, Hyung-Soo    (Department of Pharmacology, College of Medicine, Chungnam National University   ); Kim, Sun-Young    (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital   ); Jeong, Seong-Soo    (Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital   ); Heo, Ho-Jin    (Department of Pharmacology, College of Medicine, Chungnam National University   ); Seok, Jeong-Ho    (Department of Pharmacology, College of Medicine, Chungnam National University   ); Lee, Choong-Jae    (Department of Pharmacology, College of Medicine, Chungnam National University  );
  • 초록

    In this study, we investigated whether sphingosine-1-phosphate, furosemide, and indomethacin affect mucin release from airway goblet cells. Confluent primary hamster tracheal surface epithelial cells were metabolically radiolabeled and chased for 30 min or 24 hr in the presence of varying concentrations of the above agents to assess the effects on $^3H-mucin$ release. Sphingosine-1-phosphate stimulated mucin release during 30 min of treatment period in a dose-dependent manner. However, furosemide and indomethacin showed no effect on both basal and stimulated mucin release during 30 min or 24 hr of treatment period. We conclude that sphingosine-1-phosphate can affect mucin release by directly acting on airway mucin-secreting cells.


  • 주제어

    Airway .   Mucin .   Furosemide .   Indomethacin .   Sphingosine-1-phosphate.  

  • 참고문헌 (16)

    1. Cummings RJ, Parinandi NL, Zaiman A, Wang L, Usatyuk PV, Garcia JG, Natarajan V. Phospholipase D activation by sphingosine- 1-phosphate regulates interleukin-8 secretion in human bronchial epithelial cells. J Biol Chem 277: 30227-30235, 2002 
    2. Kim KC, Rearick JI, Nettesheim P, Jetten AM. Biochemical characterization of mucous glycoproteins synthesized and secreted by hamster tracheal epithelial cells in primary culture. J Biol Chem 260: 4021-4027, 1985 
    3. Ko KH, Lee CJ, Shin CY, Jo M, Kim KC. Inhibition of mucin release from airway goblet cells by polycationic peptides. Am J Physiol 277: L811-L815, 1999 
    4. Tamaoki J, Chiyotani A, Kobayashi K, Sakai N, Kanemura T, Takizawa T. Effect of indomethacin on bronchorrhea in patients with chronic bronchitis, diffuse panbroncjiolitis, or bronchiectasis. Am Rev Respir Dis 145: 548-552, 1992 
    5. Kim KC, McCracken K, Lee BC, Shin CY, Jo MJ, Lee CJ, Ko KH. Airway goblet cell mucin: its structure and regulation of secretion. Eur Respir J 10: 2644-2649, 1997 
    6. Prandota J. Clinical pharmacology of furosemide in children: a supplement. Am J Ther 8: 275-289, 2001 
    7. Wasano K, Kim KC, Niles RM, Brody JS. Membrane differentiation markers of airway epithelial secretory cells. J Histochem Cytochem 36: 167-178, 1988 
    8. Rabano M, Pena A, Brizuela A, Marino A, Macarulla JM, Trueba M, Gomez-Munoz A. Sphingosine-1-phosphate stimulates cortisol secretion. FEBS Lett 535: 101-105, 2003 
    9. Ammit AJ, Hastie AT, Edsall LC, Hoffman RK, Amrani Y, Krymska VP. Sphingosine-1-phosphate modulates human airway smooth muscle cell functions that promote inflammation and airway remodeling in asthma. FASEB J 15: 1212-1214, 2001 
    10. Bianco S, Pieroni MG, Refini RM, Rottoli L, Sestini P. Protective effect of inhaled furosemide on allergen-induced early and late asthmatic reactions. N Engl J Med 321: 1069-1073, 1989 
    11. Jeffery PK. Anti-inflammatory drugs and experimental bronchitis. Eur J Respir Dis 146: 245-257, 1986 
    12. Jolly PS, Rosenfeldt HM, Milstien S, Spiegel S. The roles of sphingosine-1-phosphate in asthma. Mol Immunol 38: 1239- 1245, 2001 
    13. Kim KC, Wasano K, Niles RM, Schuster JE, Stone PJ, Brody JS. Human neutrophil elastase releases cell surface mucins from primary cultures of hamster tracheal epithelial cells. Proc Natl Acad Sci USA 84: 9304-9308, 1987 
    14. Mutschler E, Derendorf H. Drug actions. Boca Raton, Florida, CRC Press, p 410-411, 1995 
    15. Lee CJ, Paik SH, Ko KH, Kim KC. Effects of polycationic peptides on mucin release from airway goblet cells: relationship between polymer size and activity. Inflamm Res 51: 490-494, 2002 
    16. Ellis EF. Asthma in childhood. J Allergy Clin Immunol 72 (suppl): 526-539, 1985 
  • 이 논문을 인용한 문헌 (1)

    1. Lee, Jae-Woo ; Kim, Kil-Dong ; Jeon, Byeong-Kyou ; Lee, Choong-Jae 2010. "Suppression of Induced Mucin Production from Human Airway Epithelial Cells by Coumarin and Indomethacin" 약학회지 = Yakhak hoeji, 54(5): 416~421     

 저자의 다른 논문

  • HEO Ho Jin (9)

    1. 2005 "Effects of Ambroxol, S-carboxymethylcysteine, Dextromethorphan and Noscapine on Mucin Release from Airway Goblet Cells" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 9 (6): 323~326    
    2. 2005 "Effects of Diclofenac, Acetamonophen, Nimesulide and Acetylsalicylic Acid on Mucin Release from Cultured Hamster Tracheal Surface Epthelial Cells" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 13 (4): 246~250    
    3. 2005 "Effects of Phorbol Estr, Gö-6976, Ro-31-8220 and Röttlerin on Basal Mucin Release from Airway Goblet Cells" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 13 (4): 251~255    
    4. 2006 "Inhibition of Adenosine Triphosphate-stimulated Mucin Secretion from Airway Epithelial Cells by Schizandrin" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 10 (5): 251~254    
    5. 2006 "Effects of Three Compounds from Schizandrae Fructus and Uridine on Airway Mucin Secretion" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 14 (3): 166~170    
    6. 2006 "Effects of Short-term Treatment of Daidzein, Puerarin, Genistein and Tumerone on Mucin Secretion from Cultured Airway Epithelial Cells" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 14 (3): 178~182    
    7. 2006 "Phellopterin Suppresses Airway Mucin Secretion induced by Adenosine Triphosphate" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 14 (4): 202~206    
    8. 2006 "Effect of Imperatorin on Adenosine Triphosphate-stimulated Mucin Secretion from Airway Epithelial Cells" The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology 14 (4): 235~239    
    9. 2006 "Glycyrrhizin and Morroniside Stimulate Mucin Secretion from Cultured Airway Epithelial Cells" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 10 (6): 317~321    
  • 석정호 (29)

  • 이충재 (35)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
  • 대한약리학회 : 저널
  • 대한생리학회 : 저널
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기