본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

What is the Key Step in Muscle Fatty Acid Oxidation after Change of Plasma Free Fatty Acids Level in Rats?

Doh, Kyung-Oh    (Department of Physiology, Yeungnam University College of Medicine   ); Suh, Sang-Dug    (Department of Physiology, Yeungnam University College of Medicine   ); Kim, Jong-Yeon    (Department of Physiology, Yeungnam University College of Medicine  );
  • 초록

    The purpose of this study was to discern the critical point in skeletal muscle fatty acid oxidation by changing plasma free fatty acids (FFA) level in rat. In the study, 3 key steps in lipid oxidation were examined after changing plasma FFA level by acipimox. The rates of both palmitate and palmitoylcarnitine oxidation were decreased by decrease of plasma FFA level, however, carnitine palmitoyl transferase (CPT) 1 activity was not changed, suggesting CPT1 activity may not be involved in the fatty acid oxidation at the early phase of plasma FFA change. In the fasted rats, ${\beta}-hydroxy$ acyl-CoA dehydrogenase ( ${\beta}$ -HAD) activity was depressed to a similar extent as palmitate oxidation by a decrease of plasma FFA level. This suggested that ${\beta}-oxidation$ might be an important process to regulate fatty acid oxidation at the early period of plasma FFA change. Citrate synthase activity was not altered by the change of plasma FFA level. In conclusion, the critical step in fatty acids oxidation of skeletal muscles by the change of plasma FFA level by acipimox in fasting rats might be the ${\beta}-oxidation$ step rather than CPT1 and TCA cycle pathways.


  • 주제어

    Fatty acids oxidation .   Skeletal muscle .   ${\beta}-oxidation$ .   Acipimox.  

  • 참고문헌 (18)

    1. Bulow J. Lipid mobilization and utilization. Principles of Exercise Biochemistry. 1st ed. Basel, Karger, p 140-163, 1988 
    2. Dagenais GR, Tancredi RG, Zierlier KL. Free fatty acid oxidation by forearm muscle at rest, and evidence for an intramuscular lipid pool in human forearm. J Clin Invest 58: 421-431, 1976 
    3. Gollnick PD, Saltin B. Significance of skeletal muscle oxidative enzyme enhancement with endurance training: hypothesis. Clin Physiol 2: 1-12, 1982 
    4. Howald H, Hoppeler H, Claasen H, Mathieu O, Straub R. Influence of endurance training on the ultrastructural composition of the different muscle fiber types in humans. Pfluegers Arch 403: 369- 376, 1985 
    5. Kim JY, Hickner RC, Dohm GL, Houmard JA. Long- and medium-chain fatty acid oxidation is increased in exercisetrained human skeletal muscle. Metabolism 51: 460-464, 2002a 
    6. McGarry JD, Brown NF. The mitochndrial carnitine palmitoyltransferase system from concept to molecular analysis. Eur J Biochem 244: 1-14, 1997 
    7. Watt MJ, Holmes AG, Steinberg GR, Mesa JL, Kemp BE, Febbraio MA. Reduced plasma FFA availability increases net triacylglycerol degradation, but not GPAT or HSL activity, in human skeletal muscle. Am J Physiol Endocrinol Metab 287: E120- E127, 2004 
    8. Piatti PM, Monti LD, Davis SN, Conti M, Brown MD, Pozza G, Alberti KG. Effects of an acute decrease in non-esterified fatty acid levels on muscle glucose utilization and forearm indirect calorimetry in lean NIDDM patients. Diabetologia 39: 103-112, 1996 
    9. Kim JY, Koves TR, Yu GS, Gulick T, Cortright RN, Dohm GL, Muoio DM. Evidence of a malonyl-CoA-insensitive carnitine palmitoyltransferase I activity in red skeletal muscle. Am J Physiol Endocrinol Metab 282: E1014-E1022, 2002b 
    10. Van der Vusse GJ, Reneman RS. Lipid metabolism in muscle. In: Rowell LB, Shepherd JT ed, Handbook of Physiology. 1st ed. Oxford University Press, New York, p 952-994, 1996 
    11. Zierz S, Engel AG. Different sites of inhibition of carnitine palmitoyltransferase by malonyl-CoA, and by acetyl-CoA and CoA, in human skeletal muscle. Biochem J 245: 205-209, 1987 
    12. Scholte HR, Yu Y, Ross JD, Oosterkamp II, Boonman AM, Busch HF. Rapid isolation of muscle and heart mitochondria, the lability of oxidative phosphorylation and attempts to stabilize the process in vitro by taurine, carnitine and other compounds. Mol Cell Biochem 174: 61-66, 1997 
    13. Srele PA. Citrate synthase. Meth Enzymol 13: 3-26, 1969 
    14. Donati A, Cavallini G, Carresi C, Gori Z, Parentini I, Bergamini E. Anti-aging effects of anti-lipolytic drugs. Exp Gerontol 39: 1061-1067, 2004 
    15. McGarry JD, Mills SE, Long CS, Foster DW. Observations on the affinity for carnitine, and malonyl-CoA sensitivity, of carnitine palmitoyltransferase I in animal and human tissues. Demonstration of the presence of malonyl-CoA in non-hepatic tissues of the rat. Biochem J 214: 21-28, 1983 
    16. Tornvall P, Walldius GW. A comparison between nicotinic acid and acipimox in hypertriglyceridaemia--effects on serum lipids, lipoproteins, glucose tolerance and tolerability. J Intern Med 230: 415-421, 1991 
    17. Kim JY, Hickner RC, Cortright RL, Dohm GL, Houmard JA. Lipid oxidation is reduced in obese human skeletal muscle. Am J Physiol Endocrinol Metab 279: E1039-E1044, 2000 
    18. Randle PJ. Regulatory interactions between lipids and carbohydrates: the glucose fatty acid cycle after 35 years. Diabetes Metab Rev 14: 263-283, 1998 

 저자의 다른 논문

  • 도경오 (5)

    1. 2003 "Fuctional Relationship between Rate of Fatty Acid Oxidation and Carnitine Palmitoyl Transferase I Activity in Various Rat Tissues" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 7 (4): 207~210    
    2. 2005 "Insulin Resistance of Skeletal Muscle was Recovered by Leptin Injection in vivo, but not in vitro, in High-fat Diet Fed Rats" The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 9 (2): 125~130    
    3. 2010 "장환형 단일가닥 DNA를 이용한 암세포 성장 억제 유전자 발굴" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology 38 (1): 70~76    
    4. 2010 "The Effect of Metformin on Liver Lipid Accumulation in Mice Fed a High-fat Diet" Journal of the Korean Society for Applied Biological Chemistry 53 (2): 198~205    
    5. 2015 "Validation of Heterodimeric TAT-NLS Peptide as a Gene Delivery Enhancer" Journal of microbiology and biotechnology 25 (6): 788~794    
  • 서상덕 (3)

  • 김종연 (40)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
  • 대한약리학회 : 저널
  • 대한생리학회 : 저널
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기