본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Natural product sciences v.11 no.1, 2005년, pp.22 - 26   피인용횟수: 1

Free Radical Scavenging and Lipid Peroxidation Inhibition Potential of Hygrophila auriculata

Vijayakumar, Madhavan    (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute   ); Govindarajan, Raghavan    (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute   ); Shirwaikar, Arun    (College of Pharmaceutical sciences, MAHE   ); Kumar, Vivek    (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute   ); Rawat, Ajay Kumar Singh    (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute   ); Mehrotra, Shanta    (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute   ); Pushpangadan, Palpu    (Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute  );
  • 초록

    Hygrophila auriculata (K. Schum.) Heine is a wild herb commonly found in moist places on the banks of tanks, ditches and paddy fields throughout India and is one of the main sources of Ayurvedic 'Rasayana' drug. The free radical scavenging potential of 50% aqueous alcoholic extract of H. auriculata (HAEt) was studied for DPPH scavenging activity, nitric oxide, hydroxyl radical and ferryl bipyridyl complex scavenging activity along with lipid peroxidation and total antioxidant capacity inhibition using Thiobarbituric acid reactive substances (TBARS) in rat liver homogenate. Integral antioxidative capacity was determined by photochemiluminescence assay. HAEt showed good radical scavenging activity at various concentrations $(200-1000\;{\mu}g/ml)$ against 1,1 diphenyl, 2-picrylhydrazyl (DPPH) (32.32-77.02%) with moderate scavenging activity against Nitric oxide (12.46-52.84%), hydroxyl radical (11.69-55.26%), ferryl bipyridyl complex (17.66-58.67%) and lipid peroxidation (0.829-0.416nmoles/mg protein). The above results indicate HAEt to be very effective antioxidant. It was suggested that the varied therapeutic activities claimed for the plant in the Indian indigenous systems of medicine may be in the part due to the free radical scavenging and/ or potent antioxidant activity.


  • 주제어

    Hygrophila auriculata .   free radical scavenging .   Lipid peroxidation .   Photochemiluminescence assay.  

  • 참고문헌 (24)

    1. Delanty, N., and Dichter, M.A., Oxidative injury in the nervous system. Acta Nurologica Scandinavica 98, 145-153 (1998) 
    2. Govindarajan, R., Rastogi, S., Vijayakumar, M., Rawat, A.K.S., Shirwaikar, A, Mehrotra, S., and Pushpangadan, P., Studies on the antioxidant activities of Desmodium gangeticum. Biol. Pharm. Bull. 26, 1424-1427 (2003a) 
    3. Govindarajan, R., Vijayakumar, M., Rawat, AK.S., and Mehrotra, S., Free radical scavenging potential of Picrorhiza kurrooa Royle ex Benth, Indian J. Exp. BioI. 41, 875-879 (2003b) 
    4. Valentao, P., Fernndes, E., Canvalho, E., Andrade, P.B., Seabra, R.M., and Bastos, M.L., Antioxidant activity of Hypericum androsaenium infusion scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid. Biol. Pharm. Bull. 25, 1324-1327 (2002) 
    5. Fernando, M.R, Wickramasinghe, S.M.D., Thabrew, M.I., and Karunanayaka, E.H., A preliminary investigation of the possible, hypoglycaemic activity of Asteracanthus longifolia. J. Ethnopharmacol. 27,7-14 (1989) 
    6. Marlette, M.A., Nitric oxide: Biosynthesis and Biological significance. Trend. Biochem. Sci. 14,488 (1989) 
    7. Singh, A., and Handa, S.S., Hepatoprotective activity of graveolens and H. auriculata against Paracetamol and thioacetamide intoxication in rats. J. Ethnopharmacol. 49, 119-126 (1999) 
    8. Sreejayan, N., and Rao, M. N. A., Nitric oxide scavenging by curcuminoids, J. Pharm. Pharmacol. 49, 105 (1997) 
    9. Jayatilak, P.G., Pardanani, D.S., Murty, B.D., and Seth, A.R., Effect of an indigenous drug (speman) on accessory reproductive fucntions of mice. Indian J. Exp. Biol. 14,170-73 (1976) 
    10. Preito, P., Pineda, M., and Aguilar, M., Spectrophotometric quantitation of antioxidant capacity through the formation of a phophomolybdemnum complex: specific application to the determination of vitamin E. Anal. Biochem. 269, 337-341 (1999) 
    11. Govindarajan, R., Vijayakumar, M., Rao, Ch. v; Rawat, A.K.S., Shirwaikar, A, Mehrotra, S., and Pushpangadan, P., Antioxidant potential of Anogeissus latifolia. Biol. Pharm. Bull. 27, 12661269 (2004a) 
    12. Halliwell, B., Superoxide-dependent formation of hydroxyl radicals in the presence of iron chelates. FEBS Letters 92, 321326 (1978) 
    13. Okhawa, H., Ohishi, N., and Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction, Anal. Biochem. 95, 351 (1979) 
    14. Wiseman, H., and Halliwell, B., Damage to DNA by reactive oxygen and nitrogen species: role in inflammatory disease and progression to cancer. Biochem. J. 313, 17-29 (1996) 
    15. Tripathi, Y.B. and Sharma, M., Comparison of the antioxidant action of the alcoholic extract of Rubia cordifolia with Rubiadin, Indian J. Biochem. Biophys. 35, 313 (1998) 
    16. Thomas, C.E. and Kalayanaram, B., Oxygen radicals and the disease process, 3rd ed. Hardwood Academic Publishers, Netherland, 1999 
    17. Desmarchelier, C, Ramao, R.L., Coussio, J., and Ciccia, G., Antioxidant and free radical scavenging activities in extracts from medicinal trees used in the Caatinga region in northeastern Brazil. J. Ethnopharmacol. 67, 69-77 (1999) 
    18. Halliwell, B., and Gutteridge, J.M.C., Free radicals, aging and diseases, in Free radicals in biology and medicine, Oxford press, Oxford, 1989, pp. 419-422 
    19. Miller, M. J., Sadowska-Krowicka, H., Chotinaruemol, S., Kakkis, J. L., and Clark, D. A., Amelioration of chronic ileitis by nitric oxide synthase inhibition. J. Pharmacol. Exp. Ther. 264, 11-16 (1992) 
    20. Govindarajan, R., Vijayakumar, M., Rao, Ch. V., Vivek, Kumar, Rawat, A.K.S., and Pushpangadan, P., Action of Asparagus racemosus against streptozotocin -induced oxidative stress. Nat. Prod. Sci. 10, 177-181 (2004b) 
    21. Gutteridge, J.M.C., Age pigments and free radicals: fluorescent lipid complexes formed by iron and copper containing proteins, Biochem. Biophy. Acta 834, 144 (1985) 
    22. Blois, M.S., Antioxidant determinations by the use of stable free radical. Nature 26, 1199 (1958) 
    23. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.I., Protein determination using Folin-ciocalteau reagent. J. Biol. Chem. 193, 438-448 (1951) 
    24. Nash, T., The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. Biochem. J. 55, 416, (1953) 
  • 이 논문을 인용한 문헌 (1)

    1. Kim, Eun-Mi ; Won, Sun-Im 2009. "Functional Composition and Antioxidative Activity from Different Organs of Native Cirsium and Carduus Genera" 한국식품조리과학회지 = Korean Journal of Food & Cookery Science, 25(4): 406~414     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기