본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Natural product sciences v.11 no.2, 2005년, pp.67 - 74   피인용횟수: 1

Antihepatotoxic effect of Heat-treated Allium victorialis var. platyphyllum in $CCl_4-induced$ Rats and the Gas Chromatographic Analysis of Volatile Sulfur Substances

Park, Hee-Juhn    (Department of Botanical Resources, Sangji University   ); Jung, Hyun-Ju    (Department of Botanical Resources, Sangji University   ); Lim, Sang-Cheol    (Department of Botanical Resources, Sangji University   ); Jung, Won-Tae    (Central Research Institute, Il-Yang Pharmaceutical Co.   ); Kim, Won-Bae    (National Alpine Agricultural Experimental Station, RDA   ); Park, Kwang-Kyun    (College of Dentistry, Younsei University   ); Lee, Jin-Ha    (College of Biotechnology and Bioengineering, Kangwon National University   ); Choi, Jong-Won    (College of Pharmacy, Kyungsung University  );
  • 초록

    The ethanolic extracts of the leaves and bulbs of Allium victorialis var. platyphyllum (Liliaceae) collected from Daegwallyoung (D) and Ullung Island (U) in Korea were obtained using three different extracting methods. The first extracts, DL-1 DB-1, UL-1 and UB-1, were obtained from leaves (L) and bulbs (B) dried at $90^{\circ}C$ , respectively, and the second extracts, DL-2, DB-2, UL-2 and UB-2, were obtained by extracting the leaves and bulbs of fresh plant parts. The third extracts DL-3, DB-3, UL-3 and UB-3 were obtained by incubating leaves and bulbs at $36^{\circ}C$ . The six extracts obtained from A. victorialis var. platyphyllum at Daegwanllyoung (cultivated site) were orally administered to examine for a possible antihepatotoxic effect in $CCl_4-induced$ rats. DL-1 exhibited the most pronounced effect. The extracts inhibited serum ALT, AST, SDH, ${\gamma}-GT$ , ALP and LDH activities elevated by $CCl_4$ injection and attenuated decreased glutathione S-transferase, glutatione reductase and ${\gamma}-glutamylcysteine$ synthetase activities and a decreased hepatic glutathione. However, the extracts obtained from Ullung Is. (native site) were less active than the extracts from Daegwallyoung, suggesting that A. victorialis var. platyphyllum from the cultivated site is more useful for functional food than of native site. These results also suggest that the antihepatotoxic effect is due to a higher content of hepatic glutathione. Gas chromatography of the twelve extracts showed significantly different sulfides, disulfides or trisulfides contents belonging to volatile sulfur substances (VSS). Nine components were identified on the basis of their mass spectra, namely, dimethyl disulfide, dimethyl trisulfide, diallyl disulfide, dipropyl disulfide, allyl methyl sulfide, allyl methyl trisulfide, 2-vinyl-4H-1,3-dithiin, 3,4-dihydro-3-vinyl-1,2-dithiin, and allithiamine. Extract DL-1 had the highest VSS content. Dried plant materials contained larger amounts of the VSSs than other extracts, and the leaves contained larger amount than the bulbs. These results suggest that heat treatment increases the antiheaptotoxic ability of A. victorialis var. platyphyllum by increasing the proportion of VSSs.


  • 주제어

    Allium victorialis var.   platyphyllum .   antihepatotoxic .   glutathione .   Liliaceae .   volatile sulfur substance.  

  • 참고문헌 (24)

    1. Recknagel, R.O. and Glende, E.A., Mechanisms of carbon tetrachloride toxicity. Phar. & Ther. 43, 139-154 (1989) 
    2. Richman, P.G. and Meister, A., Regulation of ${\gamma}$-glutamylcysteine synthesis by nonallosteric feedback inhibition by glntathione. J. Biol. Chem. 1975, 250, 1422-1426 
    3. Yoo, K.O., Kim, W.B., and Park, H.J., et al., Investigation on the ultrastructure of epidemis, anatomical, palynological, and cytological characteristics of Allium victorialis var. platyphyllum collected from three different habitats. J. Kor. Soc. Hort. Sci. 39, 260-265 (1998) 
    4. Moon, K.S., Components of Herbal Medicine and uses. 671-672 (1984) 
    5. Reddy, C.C., Tu, C.P., Burgess, J.R., Ho, C.Y., Scholz, R.W., and Massaro, E.J., Evidence for the occurrence of selenium-dependent glutathione peroxidase activity in rat liver microsomes. Biochem. Biophys. Res. Commun. 101, 970-978 (1981) 
    6. Reitman, S. and Frankel, S. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am. J. Clin. Pathol. 28 56-60 (1957) 
    7. Choi, J.W., Lee, K.T., et al., Pharmacological Effects of the Allium victorialis var. platyphyllum extracts on the rats induced by streptozotocin, poloxamer-407, $CCl_4$ and Dgalactosamine. Kor. J. Pharmacogn. 34, 250-255 (2003) 
    8. Mize, C.E. and Langdon, R.G., Hepatic glutathione reductase: purification and general kinetic properties. J. Biol. Chem. 237, 1589-1593 (1962) 
    9. Noll, T. and Groot, H., The critical steady-state hypoxic conditions in carbon tetrachloride-induced lipid peroxidation in rat liver microsomes. Biochem. Biophy. Acta, 795, 356-362 (1984) 
    10. Ohkawa, H., Ohishi, N., and Yagi, K., Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358 (1979) 
    11. Lawson, L.D., Wang, Z.J., and Hughes, B.G., ${\gamma}$-glutamyl-Salkylcysteines in garlic and other Allium spp. Precursors of age-dependent trans-1-propenyl thiosulfinates. J. Nat. Prod. 54, 436-444 (1991) 
    12. Lee, K.T. and Choi, J.H., et al., Constituents and the antitumor principle of Allium victorialis var. platyphyllum. Arch. Pharm. Res. 24,44-50 (2001) 
    13. Roos, K. and A., A simplified autoanalyzer procedure for the determination of serum alkaline phosphatase activity. Clin. Chim. Acta. 13, 403-406 (1996) 
    14. Matsukawa, T., Yurugi, S., and Matsuoka, T., Products of the reaction between thiamine and ingredients of the plants of Allium genus: Detection of allithiamine and itshomologs. Science, 118, 325-327 (1953) 
    15. Meister, A., Selective modification of glutathione metabolism. Science 220, 472-477 (1983) 
    16. Nishimura, H., Takahashi, T., Wijaya, C.H., Satoh, A., and Aruga, T., Thermochmical transformation of sulfur compounds in Japanese domestic Allium, Allium victtorialis L., Biofactors. 13, 257-263 (2000) 
    17. Higuchi, O., Tateshita, K., and Nishimura, H., Antioxidative activity of sulfur-containing compounds in Allium species for human low-density lipoprotein (LDL) oxidation in vitro. J Agric. Food Chem. 51, 7208-7214 (2003) 
    18. Gaitonde, M.K., A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids. Biochem. J. 104, 627-633 (1967) 
    19. Gusovsky, F. and Daly, J.W., Mitotoxin: A unique pharmacological tool for research on calcium-dependent mechanisms. Biochem. Pharmacol. 39, 1633-1639 (1990) 
    20. Fried, R., Enzymatic and non-enzymatic assay of superoxide dismutase. Biochimie. 57, 657-660 (1975) 
    21. Klaassen, C.D. and Fitzgerald, T.J., Metabolism and biliary excretion of ethacrynic acid. J. Pharmacol. Exp. Ther. 191, 548-556 (1974) 
    22. Berga, L. and Boida, D., The quantitative colorimetric determination of LDH. Sigma Tech. Bull. 500, 80-60 (1960) 
    23. Weisner, I.S., Rawnsley, H.M., Brooks, F.P., and Senior, J.R., Sorbitol dehydrogenase in the diagnosis of liver disease. Am. J. Dig. Dis. 10, 147-151 (1965) 
    24. Szasa, F., Kinetic photometric method for serum giutamyltransferase. Clin. Chem. 15, 124-136 (1969) 
  • 이 논문을 인용한 문헌 (1)

    1. 2013. "" Natural product sciences, 19(3): 221~226     

 저자의 다른 논문

  • Park, Hee-Juhn (127)

    1. 1993 "얼레지 화피에 함유된 안토시아닌 색소의 군락지별 함량" 생약학회지 24 (3): 251~254    
    2. 1993 "자원식물로서 응용을 위한 야광나무 열매의 식물화학적 연구" 생약학회지 24 (4): 282~288    
    3. 1994 "와송의 Triterpene 및 Steroid 성분" 생약학회지 25 (1): 20~23    
    4. 1994 "얼레지잎의 성분(成分) 분리(分離) 및 동정(同定)에 관(關)한 연구(硏究)" 韓國藥用作物學會誌 = Korean journal of medicinal crop science 2 (1): 67~72    
    5. 1995 "왕고들빼기의 혈청 콜레스테롤 저하효과 및 트리테르페노이드 성분" 생약학회지 26 (1): 40~46    
    6. 1995 "해동피의 화학성분 및 생리활성" 생약학회지 26 (2): 122~129    
    7. 1995 "산씀바귀의 Triterpenoid 성분조성" 韓國藥用作物學會誌 = Korean journal of medicinal crop science 3 (2): 151~155    
    8. 1996 "고려인삼류와 유사생약중 ginsenoside 및 질소화합물의 함량 비교" 생약학회지 27 (1): 6~14    
    9. 1996 "함박꽃나무 잎으로 부터 새로운 Aporphine계 Alkaloid 성분의 분리" 생약학회지 27 (2): 123~128    
    10. 1996 "대두와 돌콩의 항산화 활성 및 성분비교" 생약학회지 27 (3): 190~195    
  • 정현주 (21)

  • 임상철 (39)

  • Lee, Jin-Ha (43)

  • 최종원 (134)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기